Western blot analysis of beta-Tubulin was performed by loading 75 µg of the indicated cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK Blocking Buffer (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated beta-Tubulin monoclonal antibody (Product # MA5-16308-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform, and washed in TBS-0.1% Tween-20. Fluorescent detection was performed using a near IR imaging system.