GAPDH Loading Control Antibody DyLight 680 MA5-15738-D680 from Thermo Fisher Scientific

Product Specs
ItemGAPDH Loading Control Antibody DyLight 680
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberMA5-15738-D680
Quantity50 µL
ApplicationsImmunocytochemistry, Western Blot
Concentration1 mg/mL
Conjugate/TagDyLight™ 680
FormatPBS with proprietary stabilizer and 0.02% sodium azide
PurityProtein A
ReactivityBacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast
TargetGAPDH Loading Control
IsotypeIgG1
CloneGA1R
HostMouse
Storage Temperature4° C, do not freeze
Antibody TypePrimary
ClonalityMonoclonal
ImmunogenRecombinant GAPDH
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
NCBI Gene AliasesGapd, GAPDS, G3PD, GAPDH-2, HSD-35, KNC-NDS6, Gapd-s, BARS-38, Gapds, GAPD2, GAPD, HEL-S-278, gapdh-2, HEL-S-162eP
Storage4°C, do not freeze

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Sigma-1 receptor regulates HIV-1 and methamphetamine-induced endothelial/pericyte barrier impairment via strain-specific inflammatory responses and mitochondrial dysregulationJournal of NeuroinflammationMarch 6, 2026Nikolai Fattakhov, Silvia Torices, Sarah Becker, Joelle-Ann Joseph, Sarah Schmidlin, Alex Ngo, Adesuwa Okoro, Oandy Naranjo, Michal Toborekagitation. Target protein levels were normalized to anti-GAPDH (Thermo Fisher Scientific, Cat. No. MA5-15738-D680). The blots were imaged using an Odyssey CLx unit (LI-COR Biosciences), and signal quantification was

(2) Ribosome association inhibits stress-induced gene mRNA localization to stress granulesGenes & DevelopmentJuly 1, 2025Noah S. Helton, Benjamin Dodd, Stephanie L. Moonstain. Membranes were blocked in 5% milk in TBST and then probed for GAPDH (Thermo Fisher Scientific MA5-15738-D680), ATF4 (Proteintech 81798-1-RR), EPRS1 (Proteintech 67712-1-IG), GADD34 (Proteintech 81250-1-RR), JUN

(3) Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis miceUltrason SonochemApril 1, 2025Tahidul Islam, Baojun Xu, Zhaoxiang BianCell Signaling Technology, Danvers, Massachusetts, U.S.A; mouse anti-ZO-1(339100) and loading control (MA515738D680) were purchased from ThermoFisher Scientific, Waltham, Massachusetts, USA; p-NFκB p65 (sc-166748), IκB-α

(4) ARID1A mutations protect follicular lymphoma from FAS-dependent immune surveillance by reducing RUNX3/ETS1-driven FAS-expressionCell Death & DifferentiationJanuary 23, 2025Martina Antoniolli, Maria Solovey, Johannes Adrian Hildebrand, Tabea Freyholdt, Carolin Dorothea Strobl, Deepak Bararia, William David Keay, Louisa Adolph, Michael Heide, Verena Passerini, et al.BioLegend (1:2000); ETS1, rabbit, clone D8O8A (#14069S), Cell Signaling (1:2000); GAPDH, mouse, clone 6C5 (#MA5-15738-D680), Thermo Fisher Scientific (1:10000). Antibody details are listed in Table S6. Uncropped Western blots

(5) Intestinal E. coli-produced yersiniabactin promotes profibrotic macrophages in Crohn's diseaseCell host & microbeJanuary 8, 2025Ahn JH, da Silva Pedrosa M, Lopez LR, Tibbs TN, Jeyachandran JN, Vignieri EE, Rothemich A, Cumming I, Irmscher AD, Haswell CJ, et al."GAPDH loading control monoclonal antibody (GA1R), DyLight™ 680 (1:5000 for WB)",Thermo Fisher Scientific,MA5-15738-D680 "Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 (1:1000 for ICC and IF)",Thermo Fisher Scientific

(6) POLE2 silencing inhibits the progression of colorectal carcinoma cells via wnt signaling axisCancer biology & therapyDecember 31, 2024Jian W, Zhang L.Abcam), anti-p-GSK3β (ab75814, 1:10000; Abcam), anti-β-catenin (ab32572, 1:5000; Abcam) and GAPDH (MA5–15738-D680, 1:3000; Invitrogen) antibody. Afterward, blots were then incubated with a horseradish peroxidase (HRP)

(7) Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer’s diseaseBrainJune 3, 2024Yingying Han, Kui Chen, Hongxiang Yu, Can Cui, Hongxia Li, Yongbo Hu, Bei Zhang, Gang Li(1:1000, Cell Signaling Technology, Cat. No. 8457S), mouse anti-GAPDH (1:3000, Invitrogen, Cat. No. MA5-15738-D680), rabbit anti-NMDAR1 (1:1000, Abcam, Cat. No. ab109182), mouse anti-NMDAR1 (1:1000, Abcam, Cat. No.

(8) Targeting Molecular Mechanisms of Obesity- and Type 2 Diabetes Mellitus-Induced Skeletal Muscle Atrophy with Nerve Growth FactorInternational journal of molecular sciencesApril 13, 2024Jun L, Ding XW, Robinson M, Jafari H, Knight E, Geetha T, Greene MW, Babu JR.

(9) Heterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAsNucleic acids researchMarch 21, 2024Fuentes Y, Olguín V, López-Ulloa B, Mendonça D, Ramos H, Abdalla AL, Guajardo-Contreras G, Niu M, Rojas-Araya B, Mouland AJ, et al.

(10) Ischemia-induced cardiac dysfunction is exacerbated in adiponectin-knockout mice due to impaired autophagy fluxClinical and translational scienceMarch 1, 2024Sung HK, Tang J, Jahng JWS, Song E, Chan YK, Lone AH, Peterson J, Abdul-Sater A, Sweeney G.

(11) SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23BMC cancerFebruary 15, 2024Wu Y, Chen W, Miao H, Xu T.(PTM Biolabs, Hangzhou, China; PTM-419), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Thermo Fisher, MA5-15738-D680), and IgG (Abcam, ab6715). Normal goat anti-rabbit (35,552) and goat anti-mouse (35,502) IgG were purchased

(12) Angiotensin 1 peptide-conjugated CdSe/ZnS quantum dots for cardiac-specific hydrogen sulfide targeted therapy in myocardial ischemia-reperfusion injuryFrontiers in pharmacologyJanuary 1, 2024Wang Q, Xue X, Wang P, Yu Y, Wang J, Jiang Q, Xiao J.#2631); Nix (1:1000, Abcam, ab109414); LC-3 (1:1000, Abcam, ab192890); and GAPDH (1:5000, Thermo Fisher, MA5-15738-D680). After washing with TBST, the membranes were incubated with horseradish peroxidase-conjugated secondary

(13) Alcohol-drinking during later life by C57BL/6J mice induces sex- and age-dependent changes in hippocampal and prefrontal cortex expression of glutamate receptors and neuropathology markersAddict NeurosciSeptember 1, 2023Szumlinski KK, Herbert JN, Mejia Espinoza B, Madory LE, Scudder SL.Enzo Life Sciences; ADI-SPA-860-D) or a mouse anti-GAPDH primary (1:1000 dilution; ThermoFisher Scientific; MA5–15738-D680). However, technical issues occurred when initially immunoblotting the PFC tissue from the female mice

(14) Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infectionFrontiers in ImmunologyDecember 8, 2022Lloyd Tanner, Jesper Bergwik, Ravi K. V. Bhongir, Manoj Puthia, Pernilla Lång, Mohamad N. Ali, Charlotte Welinder, Patrik Önnerfjord, Jonas S. Erjefält, Lena Palmberg, et al.rabbit polyclonal to TRAP5 (Cat #PA5-116970; Invitrogen, MA, USA), and rabbit anti-mouse GAPDH (Cat #MA5-15738-D680; Invitrogen 1:500) in blocking buffer. After washing with PBS-Tween, membranes were incubated with secondary

(15) The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro ApproachPharmaceuticals (Basel, Switzerland)November 5, 2022Ghiulai R, Mioc A, Racoviceanu R, Mioc M, Milan A, Prodea A, Semenescu A, Dehelean C, Barbu Tudoran L, Avram Ș, et al.Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (1: 2000 dilution). GAPDH Polyclonal Antibody (1:1000 dilution, MA5-15738-D680) was used as a loading control. All antibodies were purchased from Thermo Fisher Scientific, Inc., Waltham,

(16) Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathwayTranslational andrology and urologyAugust 1, 2022Su Y, Xu J, Chen S, Feng J, Li J, Lei Z, Qiao L, Wang Y, Zeng D.antibodies were used: Nrf2 (PA5-27882), HO-1 (PA5-77833), Bcl-2 (PA5-27094), Bax (MA5-14003), and GAPDH (MA5-15738-D680, Thermo Fisher Scientific). Statistical analysis The statistical analysis was performed using GraphPad

(17) Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin miceThe Journal of clinical investigationApril 15, 2022Zou HY, Guo L, Zhang B, Chen S, Wu XR, Liu XD, Xu XY, Li BY, Chen S, Xu NJ, et al.mouse anti HAP1 (1:1000, Invitrogen, catalog MA1-46412), mouse anti-GAPDH (1:3000, Invitrogen, catalog MA5-15738-D680), rabbit anti-BiP/GRP78 (1:1000, ABclonal, catalog AB2757054), rabbit anti-PSD95 (1:1000, Cell Signaling Technology

(18) ABCB1 limits the cytotoxic activity of TAK-243, an inhibitor of the ubiquitin-activating enzyme UBA1Frontiers in Bioscience-LandmarkJanuary 5, 2022Zhuoxun Wu, Yuqi Yang, Zining Lei, Silpa Narayanan, Jingquan Wang, Qiuxu Teng, Megumi Murakami, Suresh V. Ambudkar, Fengfeng Ping, Zhesheng ChenInc., Waltham, MA, USA). The loading control GAPDH was probed by anti-GAPDH monoclonal antibody (GA1R) (MA5-15738-D680, Thermo Fisher Scientific Inc., Waltham, MA, USA). Mouse IgG HRP conjugates (#7076, Cell Signaling Technology

(19) Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranesCell reportsMay 18, 2021Bhattacharyya R, Black SE, Lotlikar MS, Fenn RH, Jorfi M, Kovacs DM, Tanzi RE.AB_304560" anti-Tau,Cell Signaling Technologies,"Cat#A0024, RRID: AB_10013724" anti-GAPDH,Life Technologies,"Cat#MA5-15738-D680, RRID: AB_2537657" anti- beta2 Adrenergic Receptor (Rabbit),Abcam,"Cat#ab176490, RRID: AB_2861265" anti-Transferrin

(20) Multi-parametric analysis of 57 SYNGAP1 variants reveal impacts on GTPase signaling, localization, and protein stabilityAmerican journal of human geneticsJanuary 7, 2021Meili F, Wei WJ, Sin WC, Meyers WM, Dascalu I, Callaghan DB, Rogic S, Pavlidis P, Haas K.

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GAPDH Loading Control Antibody DyLight 680 from Thermo Fisher Scientific

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GAPDH Loading Control Antibody DyLight 680 from Thermo Fisher Scientific

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