Fig 1: Real-time polymerase chain reaction (RT-PCR) and western blot analysis of the expression of vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), and placental growth factor (PLGF) from Embryonic day 6 (E6) to Embryonic day 8 (E8). (A) The mRNA levels of VEGFA, VEGFR2, and PLGF in folate-deficient mice were significantly decreased compared with normal mice from E6 to E8 (** p < 0.01). Data are presented as the mean ± standard error of the mean (SEM). (B) Protein levels in folate-deficient mice were compared with normal mice. Statistical analysis of protein expression was from three independent experiments (* p < 0.05). Data are presented as the mean ± SEM. (C) Western blot analysis of three angiogenic growth factors. β-actin was used as a loading control. The protein levels of VEGFA, VEGFR2, and PLGF showed similar trends to the mRNAs levels. N: normal group, FD: folate-deficient group.
Fig 2: Immunohistochemistry staining with vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), and placental growth factor (PLGF). Decreased expression of VEGFA, VEGFR2, and PLGF was detected in the folate-deficient group. The pictures in the right column are the higher magnification images of the black boxes in the left column. Additionally, the black boxes were the uterus’s central region around the embryo. Arrow indicates each factor’s different expression and distribution between two groups. (A) VEGFA was localized to a wide area of the secondary decidual zone (PDZ) and the embryo in both normal and folate-deficient mice, but there was decreased expression in the folate-deficient group. (B) VEGFR2 was localized mainly to the VSF close to the AM region in normal mice, whereas VEGFR2 was localized mainly to part of the PDZ in folate-deficient mice. (C) PLGF had a similar localization pattern as VEGFA in normal mice but was not expressed in the embryo. However, PLGF was expressed mainly in the central region of the uterus instead of in the PDZ in folate-deficient mice. N: normal group; FD: folate-deficient group; E: embryo; L: luminal epithelium; SDZ: secondary decidual zone; M: mesometrial; AM: anti-mesometrial; VSF: vascular sinus folding. Scale bar: 500 μm (left), 200 μm (right).
Supplier Page from Abcam for Anti-PLGF antibody [RM0010-8F09]