Fig 1: Splicing complex is released from DNA damage.(A) Representative confocal images of immunofluorescence (IF) against XAB2, AQR, PRP19, or CCDC16 (green) and γH2AX (red) 1 hr after local damage (LD) induction with UV-C (60 J/m2). LDs are indicated by dashed lines. Scale bar: 3 µm. (B) Quantification of the IF signal of the different splicing proteins on the LD after different times of recovery. (C) Quantification of XAB2 signal on LD in different cell lines after different times of recovery. For both graphs, the signal from the local damage has been subtracted from the background of each cell. Error bars represent the standard error of the mean (SEM) obtained from at least 20 cells. Figure 3—source data 1.Source data for Figure 3B: quantification of splicing complex IF. Figure 3—source data 2.Source data for Figure 3C: quantification of XAB2 IF.