TfR (Transferrin R) Antibody (8D3) - Azide Free NB100-64979 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemTfR (Transferrin R) Antibody (8D3) - Azide Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberNB100-64979
ImmunogenMouse transformed endothelioma cell line t.end1
Concentration1.0 mg/ml
TargetTfR (Transferrin R)
ReactivityMouse
Clone8D3
FormatPBS, 1% Bovine Serum Albumin
Quantity0.25 mg (also 0.01 mg, 0.05 mg)
ClonalityMonoclonal
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Frozen,Functional,In vivo assay,CyTOF-ready,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
IsotypeIgG2a
HostRat
PurityProtein G purified
Research AreaCell Biology, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
StorageStore at -20C. Avoid freeze-thaw cycles
NCBI Full Gene Nametransferrin receptor
NCBI Gene AliasesIMD46, CD71, E430033M20Rik, TFR1, p90, Mtvr1, T9, TR, 2610028K12Rik, Trfr, TRFR, TFR
Antibody TypePrimary Antibodies

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: NB100-64979 recognizes native, soluble and denatured forms of murine CD71. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.

Sizes Available: 0.25 mg (also 0.01 mg, 0.05 mg)

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Questions and Answers

What applications has the TfR antibody been used for in published studies?

The TfR (Transferrin Receptor) antibody has been shown to work in applications such as Immunofluorescence, where it was utilized at a dilution of 1:100 for staining in mouse brain tissue, as reported in the literature ().

Can this antibody be used to study endothelial cells in mouse models?

Yes, the TfR antibody has been employed in studies focusing on endothelial cells, particularly in the context of vascular development and signaling pathways. For instance, it was used to investigate the role of endothelial serine palmitoyltransferase in maintaining vascular homeostasis and signaling in mouse models ().

Is the TfR antibody effective for detecting the transferrin receptor in different forms?

The TfR antibody is capable of recognizing native, soluble, and denatured forms of murine CD71, which is the transferrin receptor, making it versatile for various experimental conditions.

What is the specificity of the TfR antibody in terms of species reactivity?

The TfR antibody specifically reacts with mouse samples, as indicated by its reactivity profile. This specificity is crucial for experiments involving murine models, particularly in studies related to hematopoietic stem cell markers and immunology.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's diseaseCell reportsNovember 28, 2023Yu M, Nie Y, Yang J, Yang S, Li R, Rao V, Hu X, Fang C, Li S, Song D, et al.

(2) Optical blood-brain-tumor barrier modulation expands therapeutic options for glioblastoma treatmentNature communicationsAugust 15, 2023Cai Q, Li X, Xiong H, Fan H, Gao X, Vemireddy V, Margolis R, Li J, Ge X, Giannotta M, et al.Alexa 594/647 (A-21207, A-31573) were purchased from Fisher Scientific. Rat anti-transferrin (8D3, NB100-64979) was purchased from Novus Biologicals. Rat anti-VEGFR2 (BE0060) was purchased from Bio X Cell, Animals The

(3) Elevated PDGF-BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFRβ Shedding from PericytesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2023Liu G, Wang J, Wei Z, Fang CL, Shen K, Qian C, Qi C, Li T, Gao P, Wong PC, et al.ALPL (1:20, AF2909, R&D), MMP14 (1;100, ab51074, Abcam), Lectin (1:200, DL‐1174, Vector), TFRC (1:100, NB100‐64979, Novus) and Albumin (ab106582, 1:100, Abcam) for 24 h at 4 °C followed by corresponding fluorescence‐linked

(4) Direct association with the vascular basement membrane is a frequent feature of myelinating oligodendrocytes in the neocortexFluids and barriers of the CNSApril 3, 2023Palhol JSC, Balia M, Sánchez-Román Terán F, Labarchède M, Gontier E, Battefeld A.ab6581, RRID:AB_305579" Rat monoclonal anti-Transferrin Receptor (1:500),Novus Biologicals,"Cat #: NB100-64979, RRID:AB_962622" Rabbit polyclonal anti-AQP4 (1:5000),Sigma-Aldrich,"Cat #: HPA014784, RRID:AB_1844967" Mouse

(5) Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasiseLifeOctober 5, 2022Kuo A, Checa A, Niaudet C, Jung B, Fu Z, Wheelock CE, Singh SA, Aikawa M, Smith LE, Proia RL, et al.RRID:AB_823558,For Immunofluorescence (1:200) Antibody,Anti-TFRC (rat monoclonal),Novus Biologicals,NB100-64979; RRID: AB_962622,For Immunofluorescence (1:200) Antibody,Anti-CLDN5 (rabbit polyclonal),Invitrogen,34–1600;

(6) Specification of CNS macrophage subsets occurs postnatally in defined nichesNatureApril 1, 2022Masuda T, Amann L, Monaco G, Sankowski R, Staszewski O, Krueger M, Del Gaudio F, He L, Paterson N, Nent E, et al.

(7) Exercise plasma boosts memory and dampens brain inflammation via clusterinNatureDecember 1, 2021De Miguel Z, Khoury N, Betley MJ, Lehallier B, Willoughby D, Olsson N, Yang AC, Hahn O, Lu N, Vest RT, et al.sc-8066, Santa Cruz Biotechnology), mouse anti-SMA (1:100, F377, Sigma-Aldrich), rat anti-TFRC (1:200, NB100–64979, Novus Biologicals), lectin tomato (1:200, FL-1171–1, Vector Laboratories), rabbit anti-CLU (20 μg antibody

(8) Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombastheniaMolecular medicine reportsJune 1, 2021Li D, Peng J, Li T, Liu Y, Chen M, Shi X.

(9) Physiological blood-brain transport is impaired with age by a shift in transcytosisNatureJuly 1, 2020Yang AC, Stevens MY, Chen MB, Lee DP, Stähli D, Gate D, Contrepois K, Chen W, Iram T, Zhang L, et al.anti-Ibal (1:500, ab5076, Abcam), rabbit anti-MFSD2A (1:300, gift from C. Gu), rat anti-TFRC (1:100, Novus, NB100-64979) and rabbit anti-phospho-STAT3 (1:100, 9145S, CST). Sections were washed, stained with Alexa Fluor-conjugated

(10) An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin ReceptorInternational journal of molecular sciencesApril 23, 2020Meister SW, Hjelm LC, Dannemeyer M, Tegel H, Lindberg H, Ståhl S, Löfblom J.

(11) Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell SpecializationDevelopmental cellMarch 23, 2020Yanagida K, Engelbrecht E, Niaudet C, Jung B, Gaengel K, Holton K, Swendeman S, Liu CH, Levesque MV, Kuo A, et al.gift of David Silver, Duke-National University of Singapore), anti-TFRC (1:100, Novus Biologicals, CAT#NB100–64979), anti-LEF1 (1:100, Cell Signaling Technology, CAT#2230) and anti-VE-cadherin (1:200, R&D systems, CAT#AF1002)

(12) Erythropoietin stimulates fibroblast growth factor 23 (FGF23) in mice and menPflugers Archiv : European journal of physiologyOctober 1, 2018Daryadel A, Bettoni C, Haider T, Imenez Silva PH, Schnitzbauer U, Pastor-Arroyo EM, Wenger RH, Gassmann M, Wagner CA.

(13) A molecular atlas of cell types and zonation in the brain vasculatureNatureFebruary 22, 2018Vanlandewijck M, He L, Mäe MA, Andrae J, Ando K, Del Gaudio F, Nahar K, Lebouvier T, Laviña B, Gouveia L, et al.

(14) Comparative binding, endocytosis, and biodistribution of antibodies and antibody-coated carriers for targeted delivery of lysosomal enzymes to ICAM-1 versus transferrin receptorJournal of inherited metabolic diseaseMay 1, 2013Papademetriou J, Garnacho C, Serrano D, Bhowmick T, Schuchman EH, Muro S.

(15) In vivo performance of polymer nanocarriers dually-targeted to epitopes of the same or different receptorsBiomaterialsApril 1, 2013Papademetriou IT, Garnacho C, Schuchman EH, Muro S.

(16) Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismJuly 1, 2011Ruderisch N, Virgintino D, Makrides V, Verrey F.polyclonal L9393 (Sigma-Aldrich, St Louis, MO, USA), rat-anti-transferrin receptor (CD71) monoclonal NB100-64979 (Novus Biologicals, Cambridge, UK), goat-anti-CD98 (4F2hc) polyclonal sc-7094 (Santa Cruz Biotechnology,

(17) Targeting rat anti-mouse transferrin receptor monoclonal antibodies through blood-brain barrier in mouseThe Journal of pharmacology and experimental therapeuticsMarch 1, 2000Lee HJ, Engelhardt B, Lesley J, Bickel U, Pardridge WM.

(18) Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibodyHistochemistry and cell biologyJuly 1, 1998Kissel K, Hamm S, Schulz M, Vecchi A, Garlanda C, Engelhardt B.

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TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

TfR (Transferrin R) Antibody (8D3) - Azide Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (18)

Reviews

TfR (Transferrin R) Antibody (8D3) - Azide Free from Novus Biologicals, a Bio-Techne Brand

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