Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Integrin beta 1/CD29 Antibody (12G10) - BSA Free
Available conjugates: Available conjugates: UnconjugatedSpecificity: NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.Sizes Available: 0.1 mg (also 0.025 mg)