PerCP/Cyanine5.5 anti-mouse IFN-γ 505821 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 anti-mouse IFN-γ
CompanyBioLegend
Price $118.00Supplier Page View Company Product Page
Catalog Number505821
NCBI Gene AliasesIFN-g, If2f, Ifg
ApplicationsICFC - Quality tested
Conjugate/TagPerCP/Cyanine5.5
Quantity25 µg
ReactivityMouse
IsotypeRat IgG1, kappa
HostRat
CloneXMG1.2
Antibody TypePrimary
NCBI Full Gene Nameinterferon gamma
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ImmunogenE. coli-expressed, recombinant mouse IFN-γ
ClonalityMonoclonal
TargetIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Gallic acid chemoprevention of oral carcinogenesis is associated with HSD11β2 upregulation and immune remodelingScientific ReportsApril 27, 2026Puja Upadhaya, Felipe F. Lamenza, Ravi Ramalingam, Kishan Kumar Nyati, Sushmitha Jagadeesha, Suvekshya Shrestha, Reegan Kehres, Natalie Kazmierowicz, Sonali Dasari, Shaheer Masood, et al.with PMA/ionomycin (BioLegend) for 6 h, followed by staining with granzyme B (Cat#372208), IFN-γ (Cat#505822), IL-2 (Cat#503833), and IL-10 (Cat#505034) antibodies. Data were acquired on a FACS Celesta flow cytometer

(2) CD4+ T cell protection against murine Salmonella infection is female-specific and estrogen-dependentNature CommunicationsApril 16, 2026Shaina J. D’Souza, Rebecca M. Horowitz, Louay Bachnak, Victoria E. Immethun, Matthew S. Godwin, Charlotte M. Hankin, Vanessa M. Limbert, David L. Bauer, Alex J. Plaisance, Avery M. Burke, et al.anti-CD8-eFluor450, clone 53–6.7 (Cat no 48-0081-82); Biolegend anti-IFN-gamma-PerCP-Cy5.5, clone XMG1.2 (Cat no 505822); Biolegend anti-TNF-PE, clone MP6-XT22 (Cat no 506306); Biolegend anti-CD4-PE-Cy5, clone GK1.5 (Cat

(3) A Framework for Comparing Mouse Neoantigen ImmunogenicitybioRxivFebruary 16, 2026Peter J. Matulich, Carly N. Sprague, Victoria P. Schuster, Alyssa M. Granados, Rohan B. Chaudhari, Megan L. Burger(Invitrogen, Cat. No. 88-8824-00). Cells were then stained overnight with anti-mouse IFNγ (BioLegend, Cat. No. 505822) and TNFα (BioLegend, Cat. No. 506306) antibodies. Cells were analyzed on a BD FACSymphony™ A5 SE Cell

(4) In vivo detection of immune responses via cytokine activity labelingCellFebruary 5, 2026Guangqing Lu, Shanshan Zhang, Mengyang Feng, Eunha Kim, Daniel Cho, Jae Hyun Kim, Hannah Caris, Lev Silberstein, Gloria B. Choi, Jun R. Huh(53–6.7),Biolegend,Cat#100734;RRID: AB_2075239 PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody (XMG1.2),Biolegend,Cat#505822;RRID: AB_961359 PerCP/Cyanine5.5 anti-mouse Ly-6G Antibody (1A8),Biolegend,Cat#127616;RRID: AB_1877271 Mouse Leptin R Biotinylated Antibody

(5) An additional site mutation in MITA/STING gain-of-function mutants abolishes the autoimmune SAVI phenotypes and directs a therapeutic strategyCell InsightFebruary 1, 2026Fang-Xu Li, Sheng Liu, Zhi-Dong Zhang, Xin Shuai, Bi-Kun Xiao, Jia Yang, Defen Lu, Dandan Lin, Guijun Shang, Bo Zhong(Biolegend, 103012), anti-mouse-CD62L-Percp (Biolegend, 104432), anti-mouse IFNγ-Percp (Biolegend, 505822), anti-mouse GzmB-PE (Biolegend, 372208), anti-mouse CD4-BV421 (Biolegend, 100427), anti-mouse Sca-1-APC

(6) Palmitic acid-triggered B7H3 palmitoylation promotes immune escapeNature CommunicationsJanuary 19, 2026Zejun Rao, Changsheng Huang, Qi Wu, Mao Li, Anyi Liu, Tong Zhu, Wangshuo Yang, Lanlan Yin, Shengyu Zhu, Xiaowei She, et al.B recombinant antibody (372214, BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse IFN-γ antibody (505822, BioLegend), FITC-conjugated anti-active Caspase-3(559341, BD), PE hamster anti-mouse γδ T-cell receptor

(7) Topical Application of Artesunate Ameliorates Psoriatic Keratinization and Inflammation via Molecular Modulations Based on Both Human and Mouse ModelsACS OmegaJanuary 13, 2026Zhong-Zhou Huang, Ying-Yu Wang, Yu Xiao, Ji Yang, Wei-Guo Wan, Ping Huang, Qing Guo, He-Jian Zou, Xue Yangand permeabilization, the samples were intracellularly stained with PerCP/Cy5.5-anti-IFN-γ (cat. no. 505821, BioLegend), PE-anti-IL17A (cat. no. 506903, BioLegend), and PE Cy7-anti-FoxP3 (cat. no. 83412-77-25,

(8) Efficacy of anti-LAG3 and anti-PD-1 combination checkpoint inhibitor therapy against head and neck squamous cell carcinoma in a genetically engineered mouse modelOncoImmunologyDecember 31, 2025Felipe F. Lamenza, Peyton Roth, Puja Upadhaya, Suvekshya Shrestha, Sushmitha Jagadeesha, Natalie Kazmierowicz, Natalie Horn, Hasan Pracha, Sonali Dasari, Steve Oghumu(Cat#124324). Intracellular staining was conducted using antibodies against granzyme-B (Cat#372208), IFN-γ (Cat#505822), TNF-α (Cat# 506328), IL-10 (Cat#5050345) and perforin (Cat#154404) after 6-h stimulation with PMA

(9) Paris polyphylla enhances the anti-metastatic efficacy of maimendong decoction against lung cancerBiol Proced OnlineDecember 22, 2025Zhipeng Zhang, Jiaxuan Li, Wenfei Shi, Zujun Que, Wang Yao, Jialiang Yao, Wenji Shangguan, Weikang Zhu, Likun Liu, Jianhui TianPerCP/Cyanine5.5-granzyme (#150809), PE-perforin (#154305), PE-TNF-α (#506305), and PerCP/Cyanine5.5-IFN-γ (#505821), all purchased from BioLegend (Cambridge, UK). Recombinant anti-NK (#ab317504) and anti-CD8a (#ab316778)

(10) Single-cell multiomics reveals macrophage-derived IL-23 and CXCL9/10 drive pathogenic IFNG + IL17 + T cells in immunotherapy-related colitisJournal for ImmunoTherapy of CancerNovember 29, 2025Zhongshun Tang, Jiaolong Shi, Shuai Xu, Sheng Liu, Wenqian Yin, Yuyang Xiang, Junlin Li, Zhanke He, Xingxing Yao, Zhou Li, et al.were used after Fc blocking. For intracellular staining of Percp-cy5.5-anti-mouse IFN-γ (BioLegend Cat#505821), APC-cy7-anti-mouse IL17A (BioLegend Cat#506939), Alexa Fluor 488 anti-mouse IL-23 p19 (eBioscience,

(11) Probiotic-based oral vaccine mucosal delivery system enabling genetically encoded dual-antigen arraysNature CommunicationsNovember 22, 2025Yale Yue, Qi Xin, Yuanyuan Zhu, Dandan Zhu, Baohua Zhang, Xiyun Yan, Bing Jiang(100705), PE anti-mouse CD8α (100707), PE-anti-mouse CD4 (100408), PerCP/Cyanine5.5 anti-mouse IFN-γ (505821), APC anti-mouse CD19 (152409), APC anti-mouse F4/80 (123115), APC/Cy7 anti-mouse CD25 (101917), PE-anti-mouse

(12) Optimized multi-epitope neoantigen human cytomegalovirus vaccine based on adenovirus vectors elicits potent antiviral immunityFrontiers in ImmunologyNovember 21, 2025Shasha Jiang, Xianjuan Zhang, Heping Zhao, Fengjun Liu, Zonghui Li, Xiaoli Yang, Wenxuan Liu, Jing Lv, Yi Zhang, Yiran Zhang, et al.155015), Brilliant Violet 605-CD4 (Biolegend, cat. 116027), PerCP/Cyanine5.5-IFN-γ (Biolegend, cat. 505821), PE/Cyanine7-TNF-α (Biolegend, cat. 506323), APC-Granzyme B (Biolegend, cat. 372203), PE-Perforin (Biolegend,

(13) Metabolomic and transcriptomic profiling of HNSCC identifies AMIGO2 as a therapeutic target modulating tumor microenvironmentnpj Precision OncologyNovember 18, 2025Gan Liu, Xinfeng Yao, Yuchen Hou, Wentao Deng, Liyu Zhang, Shutong Li, Panpan Huang, Wenjiao Chang, Haiyan Huang, Lan Shi, et al.Subsequently, intracellular staining was performed with Anti-IFN-γ-PerCP/Cyanine5.5 (BioLegend, XMG1.2, Cat. 505822) and Anti-Granzyme B Recombinant-FITC (BioLegend, QA16A02, Cat. 372206). In addition, we used the related

(14) Milk-derived osteopontin influences the composition of the intestinal intraepithelial lymphocyte compartmentImmunoHorizonsNovember 9, 2025Kathleen G McClanahan, Jayden Capella, Jennifer A Gaddy, Danyvid Olivares-VillagómezBiosciences,13-0870,1:1000 GzB APC,NGZB,Invitrogen,17-8898,1:200 IFN-γ PerCP-Cy5-5,XMG1.2,BioLegend,505821,1:200 Ki67 APC,16A8,BioLegend,652405,1:200 Streptavidin BV421,–,BioLegend,405226,1:200 TCR-β Alexa

(15) Annonacin induces apoptosis and modulates programmed death-ligand 1 and interferon-gamma expression in triple-negative breast cancer: Integrated in silico and in vitro analysisVeterinary WorldAugust 9, 2025Retina Yunani, Sri Agus Sudjarwo, Mustofa Helmi Effendi, Rochmah Kurnijasanti, Nurul HidayahAllo-phycocyanin-A-conjugated anti-PD-L1 (E-AB-F1132E, Elabscience, USA) and FITC/PerCP-Cy5.5-conjugated anti-IFN-γ (505822, BioLegend, USA) following manu-facturer protocols. Fluorescence-minus-one and isotype controls were

(16) ABCC10-mediated cGAMP efflux drives cancer cell radiotherapy resistanceCell death and differentiationAugust 6, 2025Zhang Z, Gao J, Liao X, Zhang Z, Cao X, Gong Y, Chen W, Zhang L, Tsai HI, Wang D, et al.(BioLegend, #103108, 1:200), anti-CD8a PE (BioLegend, #100708, 1:200), anti-IFNγ Percp/Cy5.5 (BioLegend, #505822, 1:200), and anti-TNFα BV650 (BioLegend, #506333, 1:200). Metabolic CRISPR/Cas9 sgRNA library screen The

(17) Targeting the CD40 costimulatory receptor to improve virotherapy efficacy in diffuse midline gliomasCell Reports MedicineJuly 15, 2025Sara Labiano, Javier Marco-Sanz, Iker Ausejo-Mauleon, Virginia Laspidea, Reyes Hernández-Osuna, Marc Garcia-Moure, Daniel de la Nava, Sara Nuin, Marisol Gonzalez-Huarriz, Timothy N. Phoenix, et al.anti-mouse ki67,BioLegend,Cat# 100510; RRID: AB_312713 PerCP/Cy5.5 anti-mouse IFN-y,BioLegend,Cat#505821 ; RRID:AB_961361 APC anti-mouse TNF-a,BioLegend,Cat#506307; RRID: AB_315428 "Granzyme B monoclonal

(18) Augment proteasome inhibitor efficacy activates CD8+ T cell-mediated antitumor immunity in breast cancerCell Rep MedJuly 15, 2025Dongyang Tang, Shiqi Lin, Jingbo Zhou, Josh Haipeng Lei, Fangyuan Shao, Heng Sun, Xiangpeng Chu, Ling Li, Lin He, Yunfeng Qiao, et al.Antibody,Biolegend,Cat#: 125506; RRID: AB_1227705 PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody,Biolegend,Cat#: 505822; RRID: AB_961359 Brilliant Violet 605™ anti-mouse/human CD44 Antibody,Biolegend,Cat#: 103047; RRID:

(19) Biologically targeted dual adaptive and innate nano-Immunotherapy for clear cell renal cell carcinoma treatmentMol CancerJune 18, 2025Kin Man Au, Siqing Li, Tian Zhang, Andrew Z. Wangpermeabilization, they were stained with PerCP/Cyanine5.5-labeled anti-mouse IFN-γ (clone: XMG1.2, catalog number: 505822, BioLegend). The stained cells were washed before being analyzed in a BD LSR II flow cytometer. In

(20) Lysine-specific demethylase 1 controls key OSCC preneoplasia inducer STAT3 through CDK7 phosphorylation during oncogenic progression and immunosuppressionInt J Oral SciApril 17, 2025Amit Kumar Chakraborty, Rajnikant Dilip Raut, Kisa Iqbal, Chumki Choudhury, Thabet Alhousami, Sami Chogle, Alexa S. Acosta, Lana Fagman, Kelly Deabold, Marilia Takada, et al.antibody (#124321). For internal staining with PerCP/Cyanine5.5 anti-mouse IFN-γ Antibody (BioLegend #505822), the cells were fixed and permeabilized using a BD Bioscience fixation/permeabilization kit (#554714)

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(1)

5/29/2020Very Good Antibody for IFNg from BioLegend Hong SunVery good antibody.

PerCP/Cyanine5.5 anti-mouse IFN-γ from BioLegend

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PerCP/Cyanine5.5 anti-mouse IFN-γ from BioLegend

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