Anti-HIF-1 alpha antibody [EP1215Y] ab51608 from Abcam

Product Specs
ItemAnti-HIF-1 alpha antibody [EP1215Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab51608
Antibody TypePrimary
IsotypeIgG
ClonalityMonoclonal
CloneEP1215Y
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostRabbit
Conjugate/TagUnconjugated
Research AreaEpigenetics
Gene NameHIF1A
TargetHIF-1 alpha
ReactivityHuman (Hu)
Quantity10 UL, 40 UL, 100 UL

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Hepatitis B Virus-Encoded MicroRNA (HBV-miR-3) Inhibits FIH-1 Expression to Promote Tumor Angiogenesis in HBV-Related Hepatocellular CarcinomaJ Hepatocell CarcinomaDecember 27, 2023Han Chen, Dan Cao, Ning Han, Mingming Zhang, Wei Jiang, Xin Wang, Qinmin Zeng, Hong Tangantibodies were used for Western blotting: anti-VEGFA monoclonal, ab214424 (Abcam); anti-HIF-1α monoclonal, ab51608 (Abcam); Anti-HIF1AN/FIH-1 monoclonal, ab233141 (Abcam), and anti-β-actin monoclonal (Zsbio, Beijing,

(2) AMPK-HIF-1α signaling enhances glucose-derived de novo serine biosynthesis to promote glioblastoma growthJournal of experimental & clinical cancer research : CRDecember 15, 2023Yun HJ, Li M, Guo D, Jeon SM, Park SH, Lim JS, Lee SB, Liu R, Du L, Kim SH, et al.antibodies for PSAT1 (#ab96136, 1:1,000 for immunoblotting, 1:100 for immunohistochemistry) and HIF-1α (#ab51608, 1:1,000 for immunoblotting, 1:100 for immunohistochemistry) were purchased from Abcam (Cambridge, MA).

(3) GATA6-AS1 suppresses epithelial-mesenchymal transition of pancreatic cancer under hypoxia through regulating SNAI1 mRNA stabilityJ Transl MedDecember 6, 2023Yunhui Zhou, Xinyi Zhou, Qiwen Ben, Ningning Liu, Jiahui Wang, Yongpeng Zhai, Yichen Bao, Lin Zhou(Millipore). Primary antibodies against: ETS1 (ab238645, Abcam), hypoxia-inducible factor 1 alpha (HIF1A, ab51608, Abcam) and 2 alpha (HIF2A, ab243861, Abcam), E-cad (ab40772, Abcam), Vim (ab8069, Abcam), ZEB1 (ab245283,

(4) HIF-1α-Induced Mitophagy Regulates the Regenerative Outcomes of Stem Cells in Fat TransplantationCell TransplantationNovember 27, 2023Kai Zhang, Dan Jin, Xin Zhao, Bin Lu, Weiwei Guo, Rui Ren, Simo Wu, Junrui Zhang, Yunpeng Lirepair. Then, antigen blocking (ZSGB-Bio, ZLI9056) and primary antibody staining (HIF-1α; Abcam, UK, ab51608, 1:100; light chain 3 beta [LC3B], CST, USA, 3868s, 1:200; Beclin-1, Abcam, ab210498, 1:100, cell adhesion

(5) Hypoxia-Induced Biosynthesis of the Extracellular Matrix Molecules, Perlecan and Fibronectin, Promotes the Growth of Pleomorphic Adenoma Cells In Vitro ModelsBiomedicinesNovember 6, 2023Satoshi Maruyama, Manabu Yamazaki, Tatsuya Abé, Jun Cheng, Takashi Saku, Jun-ichi Tanumafor hypoxia, for 2 days (48 h). 2.2. Antibodies Rabbit monoclonal antibodies against HIF-1α (Abcam, ab51608, Cambridge, UK, 1:500) and polyclonal antibodies against the mouse basement membrane-type perlecan core

(6) scRNA‐seq and proteomics reveal the distinction of M2‐like macrophages between primary and recurrent malignant glioma and its critical role in the recurrenceCNS Neuroscience & TherapeuticsNovember 1, 2023Guiting You, Zhenyu Zheng, Yulong Huang, Guifen Liu, Wei Luo, Jianhuang Huang, Longjin Zhuo, Binghua Tang, Shunyi Liu, Caihou LinHIF‐1α protein by Immunohistochemistry (IHC) using an anti‐human HIF‐1α monoclonal antibody (Abcam, ab51608), following the manufacturer's protocol. The antibody was diluted at a ratio of 1/100. We used the Image‐Pro

(7) Systematic analysis of integrated bioinformatics to identify upregulated THBS2 expression in colorectal cancer cells inhibiting tumour immunity through the HIF1A/Lactic Acid/GPR132 pathwayCancer Cell InternationalOctober 27, 2023Ye Liu, Chunhui Jiang, Chunjie Xu, Lei Guincubated with primary antibodies against THBS2 (1:1,000; ab112543; Abcam, Cambridge, UK), HIF1 (1:500; ab51608; Abcam, Cambridge, UK), lamin B (1:500; ab32535; Abcam, Cambridge, UK) and GAPDH (1:1000; ab8245; Abcam,

(8) Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell modelFrontiers in PhysiologyOctober 16, 2023Sirsendu Jana, Tigist Kassa, Francine Wood, Wayne Hicks, Abdu I. AlayashNitrocellulose membranes were processed with different specific primary antibodies, e.g., anti-HIF-1α (ab51608), anti-β actin (ab8227), anti-band 3 (ab108414), anti-phosphotyrosine (ab179530), and anti-phospho (Y359)–band

(9) Potential therapeutic target secretogranin II might cooperate with hypoxia‐inducible factor 1α in sunitinib‐resistant renal cell carcinomaCancer ScienceOctober 1, 2023Wataru Fukumoto, Hirofumi Yoshino, Shin‐Ichi Horike, Issei Kawakami, Motoki Tamai, Junya Arima, Ichiro Kawahara, Akihiko Mitsuke, Takashi Sakaguchi, Satoru Inoguchi, et al.for immunoblotting were the anti‐chromogranin C Ab (1:3000, GTX12241; Gene Tex), the anti‐HIF1α Ab (1:1000, ab51608; Abcam), the anti‐VEGF Ab (1:250, GTX102643; Gene Tex), and the anti‐β‐actin Ab (1:5000, bs‐0061R; Bioss).

(10) Laser-activatable oxygen self-supplying nanoplatform for efficiently overcoming colorectal cancer resistance by enhanced ferroptosis and alleviated hypoxic microenvironmentBiomaterials ResearchSeptember 23, 2023Hao Jiang, Hailong Tian, Zhihan Wang, Bowen Li, Rui Chen, Kangjia Luo, Shuaijun Lu, Edouard C. Nice, Wei Zhang, Canhua Huang, et al.detection kit (Enzo Life Sciences Inc., Farmingdale, USA). Anti-GPX4 antibody (ab219592), anti-HIF-1 (ab51608), and anti-NOX2 antibody (ab129068) were obtained from Abcam (Cambridge, UK). Anti-SOD1 (SC11407) antibody

(11) The accordion technique enhances bone regeneration via angiogenesis factor in a rat distraction osteogenesis modelFrontiers in PhysiologySeptember 8, 2023Kai Liu, Sulong Wang, Ainizier Yalikun, Peng Ren, Aihemaitijiang Yusufustaining involved the use of specific antibodies, including anti-hypoxia-inducible factor (HIF)-1α (ab51608, 1: 100, Abcam, Cambridge, UK), anti-vascular endothelial growth factor (VEGF) (sc57496, 1:100, Santa

(12) Reduced expression of phosphorylated ataxia-telangiectasia mutated gene is related to poor prognosis and gemcitabine chemoresistance in pancreatic cancerBMC CancerSeptember 6, 2023Jingyu Xun, Hideo Ohtsuka, Katsuya Hirose, Daisuke Douchi, Shun Nakayama, Masaharu Ishida, Takayuki Miura, Kyohei Ariake, Masamichi Mizuma, Kei Nakagawa, et al.Cambridge, UK); anti-phospho S1981-ATM (1:50 000; Cat. No. ab81292, Abcam); anti-HIF1α (1:1000; Cat. No. ab51608, Abcam); anti-phospho-HIF1α (1:1000; Cat. No. AF0062, Funakoshi, Tokyo, Japan); anti-Met (1:1000; Cat.

(13) Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolutioniScienceAugust 18, 2023Deng Z, Rong S, Gan L, Wang F, Bao L, Cai F, Liao Z, Jin Y, Feng S, Feng Z, et al.YB1 antibody,Abcam,Cat#ab76149; RRID:AB_2219276 Rabbit monoclonal to HIF-1 alpha antibody,Abcam,Cat#ab51608; RRID:AB_880418 Rabbit monoclonal to Fos B antibody,Abcam,Cat#ab184938; RRID:AB_2721123 Mouse monoclonal

(14) Cobalt-containing borate bioactive glass fibers for treatment of diabetic woundJ Mater Sci Mater MedAugust 2, 2023Minhui Zhang, Aihua Yao, Fanrong Ai, Jian Lin, Qingge Fu, Deping Wangwith 5% non-fat milk, the blots were incubated overnight with primary antibodies of anti-human HIF-1α (ab51608, 1:1000, Abcam) and VEGF (ab51745, 1:2000, Abcam), respectively, and GAPDH (ab9484, 1:1000, Abcam) as

(15) Long Noncoding RNA AC078850.1 Induces NLRP3 Inflammasome-Mediated Pyroptosis in Atherosclerosis by Upregulating ITGB2 Transcription via Transcription Factor HIF-1αBiomedicinesJune 16, 2023Yu Tian, Qiqi Luo, Kun Huang, Tingting Sun, Shanshun LuoThe membrane was incubated with the following primary antibodies: HIF-1α (1:1000 in 5% BSA in TBST, ab51608, Abcam, Cambridge, UK), ITGB2 (1:1000 in 5% BSA in TBST, 10554-1-AP, Proteintech, Wuhan, China), NLRP3

(16) Hypoxia‐inducible factor 1A inhibition overcomes castration resistance of prostate tumorsEMBO Molecular MedicineJune 7, 2023Julie Terzic, Mohamed A Abu el Maaty, Régis Lutzing, Alexandre Vincent, Rana El Bizri, Matthieu Jung, Céline Keime, Daniel MetzgerAndrogen receptor (Abcam ab108341; dilution 1:200), TGM2 (CST, 3557; dilution 1:100), HIF1A (Abcam, ab51608; dilution 1:100), Ki‐67 (Thermo Fisher Scientific, MA5‐14520; dilution 1:200), cleaved caspase 3 (CST

(17) ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activityInternational Journal of Biological SciencesMay 11, 2023Fangyuan Dong, Hui Li, Limin Liu, Lin-Li Yao, Jiaofeng Wang, Danni Xiang, Jianxia Ma, Gansheng Zhang, Shan Zhang, Jun Li, et al.Cell Signaling Technology, #9449), cleaved caspase 3 (1:400, Cell Signaling Technology, #9661), HIF1α (1:200, Abcam, ab51608), and p-SHP2 (1:200, Abcam, ab75818). Scoring was conducted based on the percentage of positive staining

(18) Sodium selenite preserves rBM-MSCs’ stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathwayBMC Complementary Medicine and TherapiesApril 25, 2023Bahareh Rahimi, Mohammad Panahi, Hajie Lotfi, Mostafa Khalili, Astireh Salehi, Neda Saraygord-Afshari, Effat AlizadehSigma Aldrich, MO, USA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated in the anti-HIF-1α (Cat No: ab51608, Abcam), anti-GPX (Cat No: ab96257, Abcam), anti-AKT (Cat No: 680302, Biolegend), anti-Nrf2 (Cat No:

(19) CEBPD is a master transcriptional factor for hypoxia regulated proteins in glioblastoma and augments hypoxia induced invasion through extracellular matrix-integrin mediated EGFR/PI3K pathwayCell death & diseaseApril 14, 2023Mao XG, Xue XY, Lv R, Ji A, Shi TY, Chen XY, Jiang XF, Zhang X.Then the lysates were incubated at 4 °C overnight with the target-specific antibody HIF1A (Abcam, #ab51608) or CEBPD (Invitrogen, PA5-30262), or negative control of normal Rabbit and Antibody. DNA was eluted

(20) Inhibition of IDH3α Enhanced the Efficacy of Chemoimmunotherapy by Regulating Acidic Tumor MicroenvironmentsCancersMarch 16, 2023Zhang L, Song Y, Dai X, Xu W, Li M, Zhu Y.4 °C with primary antibodies: anti-IDH3α (15909-1-AP, Proteintech, Chicago, IL, USA), anti-HIF-1α (ab51608, Abcam, Cambridge, UK), anti-E-cadherin (60335-1-Ig, Proteintech), anti-N-cadherin (66219-1-Ig, Proteintech),

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8/9/2023Anti-HIF-1 alpha Antibody From Abcam With Great Signal Dan ZhaoI used this antibody to check HIF1A expression in colorectal cancer cell lines, Colo205 and HT29. I used 40ug of proteins. Running was done at 80-100v for 2h and wet transfer was done at 100v for 1.5h... Read Review

Anti-HIF-1 alpha antibody [EP1215Y] from Abcam

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Anti-HIF-1 alpha antibody [EP1215Y] from Abcam

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