Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) ab6729 from Abcam

Product Specs
ItemRabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab6729
Quantity1 mg
Conjugate/TagAlkaline Phosphatase
HostRabbit
ApplicationsDot, ELISA, ICC/IF, IHC-Fr, IHC-P, IM, WB
FormatThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Concentration1 mg/ml
ClonalityPolyclonal
Antibody TypePrimary Antibody
Research AreaImmunology & Infectious Disease
TargetIghg1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Tert-Butylhydroquinone (TBHQ) Suppresses LPS- and Poly (I:C)-Induced RAW 264.7 Macrophage Activation Through Reduced NF-κB/Type 1 Interferon and Enhanced Antioxidant-Related PathwaysToxicsOctober 16, 2025Alyssa M. Whisel, Charles D. Ricefollowed by secondary antibody incubation with rabbit anti-mouse IgG-AP diluted 1:2000 in PBS-TW20 (#AB6729, Abcam, Waltham, MA, USA) or goat anti-rabbit IgG-AP (ThermoFisher, #A-10931) diluted 1:2000 for 1.5

(2) The Non-Linear Profile of Aging: U-Shaped Expression of Myostatin, Follistatin and Intermediate Signals in a Longitudinal In Vitro Murine Cell Sarcopenia ModelProteomesNovember 22, 2024Alonso-Puyo J, Izagirre-Fernandez O, Crende O, Seco-Calvo J, Fernandez-Atutxa A, Fernandez-Lazaro D, Garcia-Gallastegi P, Sanz B.anti-Rps6kb (1:100, sc-377529). The secondary antibodies used were anti-mouse -HRP 1:2000 in TBST-BSA 5% (Ab6729, Abcam, Cambridge, UK) and anti-rabbit-HRP 1:2000 in TBST-BSA 5% (BA1054, Boster Bio, Pleasanton, CA,

(3) Resource-aware construct design in mammalian cellsNature CommunicationsJune 16, 2023Roberto Di Blasi, Mara Pisani, Fabiana Tedeschi, Masue M. Marbiah, Karen Polizzi, Simone Furini, Velia Siciliano, Francesca Ceroni(Life Technologies, Goat anti-Rat IgG A18868, polyclonal, and Abcam Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) ab6729, dilutions 1:10000) were detected using the Novex™ AP Chromogenic Substrate (Invitrogen). Image J48

(4) Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable ToxinVaccines (Basel)February 4, 2022Ephrem Debebe Zegeye, Yuleima Diaz, Pål PuntervollAfter washing 3 times with PBS-T, 100 µL 1:4000 alkaline phosphatase-labelled rabbit anti-mouse IgG (ab6729, Abcam) was added to each well and incubated for 1 h, followed by another 3 washes with PBS-T. Next,

(5) BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancersScience advancesSeptember 1, 2019Kuznetsov JN, Aguero TH, Owens DA, Kurtenbach S, Field MG, Durante MA, Rodriguez DA, King ML, Harbour JW.antibody for 24 hours and then incubated with alkaline phosphatase (AP)–conjugated secondary antibody (ab6729, Abcam, Cambridge, MA). Color was developed using a combination of nitro blue tetrazolium (NBT) chloride

(6) High-Density Redox Amplified Coulostatic Discharge-Based Biosensor ArrayIEEE Journal of Solid-State CircuitsDecember 19, 2018Alexander C. Sun, Enrique Alvarez-Fontecilla, A. G. Venkatesh, Eliah Aronoff-Spencer, Drew A. Hallone hour. For the secondary antibody, 1 μg of rabbit anti-mouse secondary antibodies linked with ALP (ab6729, Abcam) was used. Lastly, 6 mM pAPP substrate (sc-281392, Santa Cruz Biotechnology) in a 0.1 M glycine

(7) Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquensScientific ReportsDecember 1, 2018Jungho Jang, Byoung Wook Jeon, Yong Hwan KimAnti-6x His tag antibody (ab18184, ABCAM) (1:1000 dilution), and the secondary antibody was rabbit anti-mouse (ab6729, ABCAM) (1:2000 dilution). For the MeFDH1 beta subunit, a customized primary antibody was used (ABFRONTIER)

(8) Purification and Characterization of Native and Vaccine Candidate Mutant Enterotoxigenic Escherichia coli Heat-Stable ToxinsToxins (Basel)July 3, 2018Morten L. Govasli, Yuleima Diaz, Ephrem Debebe Zegeye, Christine Darbakk, Arne M. Taxt, Pål Puntervollused in all dilutions. 100 µL ELISA PBS-T containing a secondary anti-mouse antibody (Product code: ab6729 (Abcam, Cambridge, UK)) linked to alkaline phosphate was then added to a final dilution of 1:4000. After

(9) Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed miceBiochimica et biophysica acta. Molecular and cell biology of lipidsMarch 1, 2018Milligan S, Martin GG, Landrock D, McIntosh AL, Mackie JT, Schroeder F, Kier AB.(St. Louis, MO). Alkaline phosphataseconjugated rabbit polyclonal antibody to mouse IgG (product # ab6729-I) was from Abcam (Cambridge, MA). Antibodies to housekeeper proteins were obtained as follows: Mouse monoclonal antibody

(10) Modified human glucagon-like peptide-1 (GLP-1) produced in E. coli has a long-acting therapeutic effect in type 2 diabetic micePLOS ONEJuly 27, 2017Fangfang Xu, Kevin Yueju Wang, Nan Wang, Gangqiang Li, Dehu Liurabbit anti-mouse immunoglobulin G-alkaline phosphatase (IgG-AP) as the secondary antibody (1.00 mg/mL, ab6729, Abcam Ltd, Cambridge, United Kingdom), with a dilution of 1: 2000 and 1: 3000, respectively. The immuno-labeled

(11) Effect of Fabp1/Scp-2/Scp-x Ablation on Whole Body and Hepatic Phenotype of Phytol-Fed Male MiceLipidsMay 1, 2017Landrock D, Milligan S, Martin GG, McIntosh AL, Landrock KK, Schroeder F, Kier AB.(St. Louis, MO). Alkaline phosphatase-conjugated rabbit polyclonal antibody to mouse IgG (product # ab6729-I) was from Abcam (Cambridge, MA). Protein levels were quantified as described previously (31). Where

(12) Impact of dietary phytol on lipid metabolism in SCP2/SCPX/L-FABP null miceBiochimica et biophysica acta. Molecular and cell biology of lipidsMarch 1, 2017Milligan S, Martin GG, Landrock D, McIntosh AL, Mackie JT, Schroeder F, Kier AB.(St. Louis, MO). Alkaline phosphatase-conjugated rabbit polyclonal antibody to mouse IgG (product # ab6729-I) was obtained from Abcam (Cambridge, MA). Mouse monoclonal antibody to mouse GAPDH (MAB374) was purchased

(13) Relative contributions of L-FABP, SCP-2/SCP-x, or both to hepatic biliary phenotype of female miceArchives of biochemistry and biophysicsDecember 15, 2015Martin GG, Landrock D, Landrock KK, Howles PN, Atshaves BP, Kier AB, Schroeder F.Manuscript Louis, MO). Alkaline phosphatase-conjugated rabbit polyclonal antibody against mouse IgG (product # ab6729-1) was purchased from Abcam (Cambridge, MA). All chemicals and solvents were the highest commercially

(14) High fat plus high cholesterol diet lead to hepatic steatosis in zebrafish larvae: a novel model for screening anti-hepatic steatosis drugsNutrition & MetabolismDecember 1, 2015Wencong Dai, Kunyuan Wang, Xinchun Zheng, Xiaohui Chen, Wenqing Zhang, Yiyue Zhang, Jinlin Hou, Li Liu1:1000, Abcam, San Franciso, USA), goat anti-rabbit-HRP (Cat.No.ab6721, 1:10000, Abcam), anti-mouse-HRP (Cat.No.ab6729, 1:10000, Abcam) were used. Quantification of band intensities was performed using Image J software. Statistical

(15) Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female miceArchives of biochemistry and biophysicsAugust 15, 2015Martin GG, Atshaves BP, Landrock KK, Landrock D, Schroeder F, Kier AB.Louis, MO). Alkaline phosphatase-conjugated rabbit polyclonal antibody against mouse IgG (product # ab6729-1) was purchased from Abcam (Cambridge, MA). One-Step PCR Master Mix reagent kit and gene-specific assays

(16) Peroxiredoxin-2 Recycling Is Inhibited During Erythrocyte StorageAntioxidants & Redox SignalingFebruary 1, 2015Victoria M. Harper, Joo Yeun Oh, Ryan Stapley, Marisa B. Marques, Landon Wilson, Stephen Barnes, Chiao-Wang Sun, Tim Townes, Rakesh P. Patelskim milk (w/v) in TBS-T (overnight, 4C) followed by rabbit anti-mouse IgG alkaline phosphatase-conjugate (ab6729; Abcam) diluted 1:1000 in 5% nonfat dried skim milk (w/v) in TBS-T for 2 h. After washing with TBS-T

(17) Chlorine gas exposure disrupts nitric oxide homeostasis in the pulmonary vasculatureToxicologyJuly 3, 2014Jaideep Honavar et al.overnight in cold room followed by rabbit anti-mouse IgG antibody, Alkaline Phosphatase-conjugate (ab6729, Abcam) diluted 1:2000 in in TBS-T at room temperature for 2h. Membrane as loading control were incubated

(18) Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cellsJournal of immunology (Baltimore, Md. : 1950)June 15, 2013Iversen R, Di Niro R, Stamnaes J, Lundin KE, Wilson PC, Sollid LM.

(19) Curcumin promotes A-beta fibrillation and reduces neurotoxicity in transgenic DrosophilaPloS oneJanuary 1, 2012Caesar I, Jonson M, Nilsson KP, Thor S, Hammarström P.washed three times for 10 minutes in TBST, incubated with alkaline phosphatase-conjugate anti-mouse IgG (ab6729-1, AbCam, Cambridge, MA, USA), diluted 1∶1 000 in 4% BSA/TBST for 30 minutes at room temperature, and

(20) The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylationMolecular Biology of the CellMay 15, 2011Hui-Yong Lian, E. Douglas Robertson, Shin-ichiro Hiraga, Gina M. Alvino, David Collingwood, Heather J. McCune, Akila Sridhar, Bonita J. Brewer, M. K. Raghuraman, Anne D. DonaldsonAbcam)—then washed, and incubated for 2 h with secondary antibody (either HRP-conjugated anti–mouse [ab6729 Lot 131853; Abcam] or HRP-conjugated anti–rabbit [12–348 Lot 29902; Upstate]) before enhanced chemiluminescence

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Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) from Abcam

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Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) from Abcam

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