Anti-mtTFA/TFAM Antibody Picoband® PA1936 from BosterBio

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Anti-mtTFA/TFAM Antibody

Western blot analysis of mtTFA using anti-mtTFA antibody (PA1936).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: huamn Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (Catalog # PA1936) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24 kDa. The expected band size for mtTFA is at 29 kDa.
Anti-mtTFA/TFAM Antibody

Anti-mtTFA/TFAM Antibody
Anti-mtTFA/TFAM Antibody

IHC analysis of mtTFA using anti-mtTFA antibody (PA1936).
mtTFA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Anti-mtTFA/TFAM Antibody

IHC analysis of mtTFA using anti-mtTFA antibody (PA1936).
mtTFA was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-mtTFA/TFAM Antibody Picoband®

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BosterBio's Anti-mtTFA/TFAM Antibody Picoband® is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot. The Anti-mtTFA/TFAM Antibody Picoband® was generated using TFAM as the antigen and it reacts with Homo Sapiens, and Human.

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Boster Bio Anti-mtTFA/TFAM Antibody catalog # PA1936. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance

Anti-mtTFA/TFAM Antibody

Anti-mtTFA/TFAM Antibody

Anti-mtTFA/TFAM Antibody

Anti-mtTFA/TFAM Antibody

Anti-mtTFA/TFAM Antibody Picoband® from BosterBio

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Anti-mtTFA/TFAM Antibody Picoband® from BosterBio

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