Fig 1: NFAT inactivation was responsible for RA-mediated inhibition on Fas L. (A-B) Western blot and representative immunofluorescence images of NFAT3 nuclear translocation of CFs (n=4). (C) Ionomycin (100nM) was added to CFs with RA to induce NFAT activation and nuclear translocation (n=5). (D-E) Expression of Fas L in CFs and its release to ConM (n=4-5). (F-K) Western blot and statistical results of CMs (n=4). (L) MMP7 protein levels in neonatal rat heart CFs with or without RA protection (n=4). (M) NFAT3 nuclear translocation data in adult mouse heart CFs by western blot (n=4). Values represent the mean±SEM. *P<0.05 versus the matched control. In figure 5A, L-M, *P<0.05 versus PBS+Veh, #P<0.05 versus DOX+Veh.