TH1L (D5G6W) Rabbit mAb 12265S from Cell Signaling Technology

Product Specs
ItemTH1L (D5G6W) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12265S
Quantity100 µl
ApplicationsW, IP, ChIP
ReactivityH, M, R, Mk
ClonalityMonoclonal
TargetTH1L
HostRabbit
NCBI Full Gene Namenegative elongation factor complex member C/D
NCBI Gene AliasesHSPC130, NELF-C, NELF-D, TH1, TH1L
Research AreaChromatin Regulation / Nuclear Function
CloneD5G6W
Molecular Weight66

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) NELF prevents transcriptional readthrough into DNA replication zones in cancer cellsEMBO ReportsFebruary 20, 2026Chihiro Nakayama, Qi Fang, Yasukazu Daigaku, Yuki Aoi, Shoko Ito, Mami Takahashi, Reo Shimatani, Tamiko Minamisawa, Yagiz Ozturk, Hiroshi Kimura, et al.room temperature. Next, primary antibodies were diluted as follows: PCNA (SC-56, Santa Cruz) and MCM2 (12265, Cell Signalling) at 1:500, and Pol II Ser2P (NB100-1805, Novus) at 1:1000. For secondary antibodies,

(2) Tripartite phosphorylation of SPT5 by CDK9 times pause release and tunes elongation rate of RNA polymerase IIMolecular CellMay 1, 2025Rui Sun, Robert P. Fisher,Cell Signaling Technology,Cat. # 14793 Rabbit monoclonal NELFCD (D5G6W) Antibody,Cell Signaling Technology,Cat. # 12265 Mouse monoclonal NELFE Antibody,Santa Cruz Biotechnology,Cat. # sc-377052 Rabbit monoclonal RPB1-pSer2

(3) TH1L involvement in colorectal cancer pathogenesis by regulation of CCL20 through the NF‐κB signalling pathwayJournal of Cellular and Molecular MedicineMay 29, 2024Shaochang Wang, Yujing Sun, Chunya Li, Yueyang Chong, Meihong Ai, Yanxia Wang, Haiyun Shi, Yu Shangmonoclonal anti‐β‐actin (1:1000, Cat# sc‐8432) from Santa Cruz (Dallas Texas, USA), anti‐TH1L (1:1000, Cat# 12265), anti‐IKKβ (1:1000, Cat# 8943), anti‐NF‐κB p65 (1:1000, Cat# 8242S) and anti‐TAK1 (1:1000, Cat# 5206S)

(4) Rapid unleashing of macrophage efferocytic capacity via transcriptional pause releaseNatureApril 1, 2024Tufan T, Comertpay G, Villani A, Nelson GM, Terekhova M, Kelley S, Zakharov P, Ellison RM, Shpynov O, Raymond M, et al.D6K9A), Cell Signaling Technology, 14894, 1:1,000; NELFCD (clone D5G6W), Cell Signaling Technology, 12265, 1:1,000; β-actin (clone AC-15), Sigma, A3854, 1:30,000; EGR1 (clone 44D5), Cell Signaling Technology,

(5) SPT6 functions in transcriptional pause/release via PAF1C recruitmentMolecular cellSeptember 15, 2022Aoi Y, Shah AP, Ganesan S, Soliman SHA, Cho BK, Goo YA, Kelleher NL, Shilatifard A.RRID:AB_309449" RTF1,Bethyl,"Cat# A301-329A, RRID:AB_937989" NELF-C,Cell Signaling Technology,"Cat# 12265, RRID:AB_2797862" β-Actin,Cell Signaling Technology,"Cat# 3700S, RRID:AB_2242334" "Chemicals, Peptides,

(6) RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunityNature communicationsApril 20, 2022Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, et al.Technology, 14894 S), anti-NELFA (Proteintech, 10456-1-AP), and anti-NELFC (Cell Signaling Technology, 12265 S). All antibodies were used at 1:1000 dilution. CAR-T generation and characterization A lentiviral

(7) SPT5 stabilization of promoter-proximal RNA polymerase IIMolecular cellNovember 4, 2021Aoi Y, Takahashi YH, Shah AP, Iwanaszko M, Rendleman EJ, Khan NH, Cho BK, Goo YA, Ganesan S, Kelleher NL, et al.RRID:AB_2242334" NELF-A,Proteintech,"Cat# 10456–1-AP, RRID:AB_2216327" NELF-C,Cell Signaling Technology,"Cat# 12265, RRID: AB_2797862" NELF-E,Abcam,"Cat# ab170104, RRID:AB_2827280" VCP,Thermo Fisher Scientific,Cat#

(8) Promoter-specific changes in initiation, elongation, and homeostasis of histone H3 acetylation during CBP/p300 inhibitioneLifeMarch 11, 2021Hsu E, Zemke NR, Berk AJ.Abcam), AF9 (GTX102835, Genetex), ENL/MLLT1 (14893, Cell Signaling), BRD4 (A301-985A50), NELF TH1L D5G6W (12265S, Cell Signaling), Pol2 Ser2-P 31Z3G (13499, Cell Signaling), Pol2 Ser5-P D9N5I (13523, Cell Signaling),

(9) Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promotersMolecular cellFebruary 18, 2021Endres T, Solvie D, Heidelberger JB, Andrioletti V, Baluapuri A, Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, et al.ab1790), Ubiquityl-Histone H2B (Cell Signaling Technology, 5546), NELFC (Cell Signaling Technology, 12265S). Chromatin was added to the beads, and IP was performed for at least 6 h at 4 °C with rotation. Beads

(10) NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-ReleaseMolecular cellApril 16, 2020Aoi Y, Smith ER, Shah AP, Rendleman EJ, Marshall SA, Woodfin AR, Chen FX, Shiekhattar R, Shilatifard A.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies TH1L (D5G6W) Rabbit mAb (NELFC/D),Cell Signaling,"Cell Signaling Technology Cat# 12265, RRID:AB_2797862" Recombinant Anti-NELFe antibody [EPR11600],Abcam,"Abcam Cat# ab170104, RRID:AB_2827280" Purified

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TH1L (D5G6W) Rabbit mAb

TH1L (D5G6W) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (10)

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TH1L (D5G6W) Rabbit mAb from Cell Signaling Technology

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