Histone H2A (D6O3A) Rabbit mAb 12349S from Cell Signaling Technology

Product Specs
ItemHistone H2A (D6O3A) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12349S
Quantity100 µl
ApplicationsW, IF-IC, ChIP
ReactivityH, M, R, Mk, Z, GP
ClonalityMonoclonal
TargetH2A.1
HostRabbit
NCBI Full Gene NameH2A clustered histone 13
NCBI Gene AliasesH2A/c, H2AC11, H2AC15, H2AC16, H2AC17, H2AFC, HIST1H2AI
Research AreaChromatin Regulation / Nuclear Function
CloneD6O3A
Molecular Weight14

Questions and Answers

Can this antibody be used for detecting histone H2A in Western blotting experiments?

Yes, the Histone H2A (D6O3A) Rabbit mAb has been utilized in Western blotting, as evidenced by its inclusion in various studies where it successfully detected histone H2A protein levels in different experimental contexts, including cancer research and cellular signaling studies.

Is this antibody effective for immunofluorescence applications?

The Histone H2A antibody has been shown to work in immunofluorescence applications, allowing for the visualization of histone H2A localization within cells. For instance, in studies involving antiviral immune responses, this antibody was used to analyze histone H2A's nuclear/cytoplasmic trafficking.

What species can this antibody be used with?

This antibody is reactive with several species, including humans, mice, rats, guinea pigs, non-human primates, and zebrafish, making it versatile for various biological research applications across different model organisms.

Are there any specific experimental conditions or modifications recommended for using this antibody effectively?

In the context of Western blotting, it has been noted that the addition of a denaturation step can significantly enhance the accessibility of histone epitopes, thereby improving the detection sensitivity of histone antibodies, including the Histone H2A antibody.

Cell Signaling Technology

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United States

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Phone: 978-867-2388
Fax: 866-432-6112
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivationJournal of VirologyApril 21, 2026Daniel E. Greaves, Ildar Gabaev, James C. Williamson, Stuart Bloor, Sergio Martinez Cuesta, Nicholas J. Matheson, Lori Frappier, Jérôme Déjardin, Paul J. Lehnerrabbit α-H3K9me3 (Abcam ab8898, used for immunoblot and FACS), rabbit α-Histone H2A (Cell Signaling #12349, used for ChIP), rabbit α-Histone H3 (Abcam ab1791, used for immunoblot and ChIP), rabbit α-phospho-Akt

(2) Enhancer of zeste homolog 2 (Ezh2) is required in mouse Isl1-expressing progenitors for proper development of cardiac and skeletal hindlimb structuresBiology OpenApril 15, 2026Hamna Ammar, Daniel B. Patolsky, Lijun Chi, Amalia N. Caballero, Paul Delgado-Olguín(Cell Signalling, 13901, 1:700), H2aK119ub (Cell Signalling, 8240, 1:800), and H2A (Cell Signalling, 12349, 1:800). The following secondary antibodies were used: mouse anti-rabbit IgG–HRP (Santa Cruz Biotechnology,

(3) Inhibition of USP7 destabilizes the noncanonical PRC1.1 complex and induces neuroblastoma differentiationMolecular Cancer ResearchMarch 19, 2026Emily A. Cmarik, Abhishek Wahi, Sayali S. Chandekar, Solomon K. Otchere, Jennifer E. Sturgis, Silvi Salhotra, Allen T. Basanthakumar, Nehemiah X. Boyd, Giulia DiGiovanni, He Zhu, et al.(Santa Cruz, sc-47724 RRID:AB_627678), HSP90α (Santa Cruz, sc-515081 RRID: AB_2943315), Histone H2A (CST, 12349S RRID: AB_2687875), and Histone H3 (CST, 3638S RRID: AB_1642229). Co-Immunoprecipitation assay Approximately

(4) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. Saldivaranti-histone H1.5,Abcam,Cat#ab18208; RRID:AB_470263 Rabbit monoclonal anti-histone H2A (D6O3A),Cell Signaling Technology,Cat#12349S; RRID:AB_2687875 Rabbit monoclonal anti-histone H2B (D2H6),Cell Signaling Technology,Cat#12364S; RRID:AB_2714167 Rabbit

(5) MYSM1-mediated epigenetic modification dysregulation leads to immunosuppression and secondary infections in sepsisPLOS PathogensFebruary 12, 2026Jiali Xiong, Xin Cheng, Xiaoxing Xiong, Heyang Zhang, Qi An, Zhiqiang Li, Hong Fan, Guangli Li, Wei Li, Mingfu Tian, et al.anti-H3K79me3 (4260S), anti-H3K9me3 (13969T), anti-H3K27me3 (9733T), anti-H3K27ac (8173T), and anti-H2A (12349S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (G9295) was purchased

(6) Fork Reversal Safeguards Epigenetic Inheritance During Replication StressResearch SquareJanuary 29, 2026Wenpeng Liu, Caixian Zhou, Caixian Zhou, Nadia I. Martin, Nadia Martin, Tae-Hee Gwit, Tae-Hee Hedglin, Roséa Hedglin, Roséa Chen, Tianpeng Zhang, et al.Fluor 488-conjugated azide (9:1 ratio). The following primary antibody pairs were used: rabbit H2A (CST 12349) (1:100 dilution), H3 (Abcam ab1791) (1:200 dilution), H3K9ac (Millipore 07-352) (1:100 dilution), H3K9me3

(7) PRSS55 regulates BCAA metabolism and interacts with BCKDK and BCKDHA in mouse testes and spermCell BiosciDecember 24, 2025Haoyang Ge, Chunling Shen, Wenfeng Xiong, Shunyuan Lu, Chaojie Li, Lingyun Tang, Yan Shen, Hongxin Zhang, Jian Fei, Zhugang WangTubulin (1:1000, 11224-1-AP, Proteintech, Wuhan, China) as a cytosolic marker, and Histone H2A (1:1000, #12349, Cell Signaling Technology) as a nuclear marker. Preparation of testicular single-cell suspension Testicular

(8) Rate-limiting enzymes in nucleotide metabolism synchronize nucleotide biosynthesis and chromatin formationMolecular CellDecember 18, 2025Shashank Srivastava, Daniela Samaniego-Castruita, Shubhi Srivastava, Sakshi Khurana, Jalees Rehman, Vipul Shukla, Issam Ben-Sahra, Daniel R. FoltzBiosciences 31–1081-00), H5K12ac (Cell Signaling Technologies13944S), H2A (Cell Signaling Technologies 12349), α-tubulin (Proteintech, 66031–1), β-actin (Sigma-Aldrich, A2228), FLAG (Sigma-Aldrich, F3165), HA

(9) Nuclear actin and DNA replication stress regulate telomere maintenance by telomeraseNat CommunDecember 2, 2025Ashley Harman, Melissa Kartawinata, Nohad M. Maroun, Darren R. Nguyen, Shabita Rahman, William E. Hughes, Kevin Winardi, Scott B. Cohen, Anthony J. Cesare, Noa Lamm, et al.(T389) (1:1000, Cell Signaling Technology, 9234S), anti-Histone 2 A (1:1000, Cell Signaling Technology, 12349S), anti-ATR (1:1000, Cell Signaling Technology, 2790S) and anti-ATRIP (1:1000, Thermo Fisher Scientific,

(10) Overexpression of ATase1 and ATase2 disrupts the secretome and causes a progeria phenotypeLife science allianceDecember 1, 2025Cheng TL, Wu F, Haque ME, Thiel AR, Wang D, Helgager JJ, Li L, Puglielli L.Cell Signaling), Lys8 (#2594, 1:1,000; Cell Signaling), Lys12 (#13944, 1:1,000; Cell Signaling), Histone H2A (#12349, 1:1,000; Cell Signaling), Histone H2B (#12364, 1:1,000; Cell Signaling), Histone H3 (#4499, 1:1,000;

(11) Distinct characteristics of VEXAS-causative UBA1 M41 and recurrent functional non-M41 mutationsLeukemiaDecember 1, 2025Maki Sakuma, Amy K. Wang, Samuel J. Magaziner, Sachiko P. Keane, Manja Meggendorfer, Wolfgang Kern, Claudia Haferlach, Torsten Haferlach, David B. Beck, Wencke Walter4330), UBE2L3 (R&D Systems, E2-640), ubiquitylated-H2A (Cell Signaling, 8240), H2A (Cell Signaling, 12349), ubiquitylated-H2B (Cell Signaling, 5546S), H2B (Cell Signaling, 12364), and β-actin (Cell Signaling,

(12) Extracellular vesicles for delivery of functional telomeraseCell Communication and SignalingNovember 26, 2025Yingying He, Yumeng Cui, Lijuan Wang, Wen Yang, Ruping Wang, Yanghua Li, Yue Ma, Yanli Lin, Xiaojie Wu, Long Cheng, et al.(diluted 1:1000, #13174, Cell Signaling Technology, Danvers, MA, USA), anti-γ-H2A (diluted 1:1000, #12349, Cell Signaling Technology, Danvers, MA, USA), CD63 (diluted 1:1000, sc-5275, Santa Cruz Biotechnology,

(13) Restoration of BAP1 activity via base editing suppresses anchorage-independent survival in kidney cancerCancer Cell InternationalNovember 25, 2025Daye Lee, Boram Lee, Jiyeon Lee, Jongbum KwonAbFrontier; anti-cyclobutane pyrimidine dimers (CPD; CAC-NM-DND-001) from Cosmo Bio; and anti-H2A (12349), anti-β-catenin (9582), anti-N-cadherin (13116), anti-vimentin (5741), and anti-Cas9 (19526) from Cell Signaling Technology

(14) A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase ActivityACS Chemical BiologyOctober 17, 2025Yi-Hsun Ho, Emma K. Seipp, Thitiwat Larndate, Rong HuangH4S1ac (homemade, 1:500), histone H4 (Proteintech 16047–1-AP, 1:1000), and histone H2A (Cell Signaling 12349S, 1:1000). The membrane was incubated with the primary antibody at 4°C overnight. After three washes

(15) Differential regulation of mesoscale chromosome conformations in osteoblasts and osteosarcomaGenome BiologySeptember 26, 2025Madhoolika Bisht, Yu-Chieh Chung, Siou-Luan He, Sydney Willey, Benjamin D. Sunkel, Meng Wang, Benjamin Z. Stanton, Li-Chun Tu(Cat#2899S), H2B-V119 (Cat#8135S), H3 (Cat#9715S), and rabbit monoclonal antibody against H2A-D603A (Cat#12349S) were obtained from Cell Signaling Technology. Rabbit polyclonal antibodies against RAD21 (Cat#27,071–1-AP),

(16) IFN‐γ‐Induced CD317 Tethers Extracellular Vesicles to Mesenchymal Stromal Cells Interfering With Immune ModulationJ Extracell VesiclesAugust 20, 2025Anton Selich, Luisa Weisskoeppel, Ralf Hass, Jan Hegermann, Adrian Schwarzer, Constantin von Kaisenberg, Axel Schambach, Michael Rothe#MA1‐23296), and histone H2A (anti‐histone H2A, rabbit monoclonal, Cell Signalling Technology, 1:1000, #12349S). For detection, the respective secondary HRP‐conjugated antibody was used (goat anti‐mouse IgG HRP,

(17) IFI16 Mediates Deacetylation of KSHV Chromatin via Interaction with NuRD and Sin3A Co-Repressor ComplexesVirusesJune 28, 2025Anandita Ghosh, Bala Chandran, Arunava Roy(Cell Signaling, 8056); CoREST anti-rabbit (Cell Signaling, 14567); Histone H2A anti-rabbit (Cell Signaling, 12349); Acetyl-Histone H2A anti-rabbit (Cell Signaling, 2576); Histone H2B anti-rabbit (Cell Signaling 12364);

(18) Nuclear SUMOylation and Proteotoxic Stress Responses to Metals with Different Ligand PreferencesChemical Research in ToxicologyMay 19, 2025Giorgiana Madalina Ursu, Casey Krawic, Anatoly Zhitkovich(Cell Signaling, 12586), K119-monoubiquitinated histone H2A (Cell Signaling, 8240), histone H2A (Cell Signaling, 12349), HSC70 (Santa Cruz, sc7298), HIF1α (BD Biosciences, 610958), Pro564-hydroxylated HIF1α (Cell Signaling,

(19) BAP1 Loss Affords Lipotoxicity Resistance in Uveal MelanomaPigment Cell Melanoma ResApril 29, 2025C. J. Cunanan, A. Amirfallah, A. B. Sanders, K. C. Gallant, M. R. Cavallo, E. A. Homer, O. S. El Naggar, J. K. Farnan, G. Romano, J. L. Hope, et al.(#2118), Tubulin (#2148), BAP1 (#13271), ASXL2 (#71257), Ubiquityl‐Histone H2A (#8240), and Histone H2A (#12349) were purchased from Cell Signaling Technology. PPARα (#398394) was purchased from Santa Cruz Biotechnology.

(20) A nucleosome switch primes hepatitis B virus infectionCellApril 17, 2025Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, et al.,Cell Signaling Technology,Cat# 3873 ACF1,Abcam,Cat# ab187670 "H2A (D6O3A), ChIP",Cell Signaling Technology,Cat# 12349 "H2A, immunoblot",Abcam,Cat# 18255 H2B,Cell Signaling Technology,Cat# 12364 H3,Abcam,Cat# ab1791 H3K4me3,Epicypher,Cat#

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Histone H2A (D6O3A) Rabbit mAb

Histone H2A (D6O3A) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Histone H2A (D6O3A) Rabbit mAb from Cell Signaling Technology

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