Anti-Nephrin antibody ab58968 from Abcam

Product Specs
ItemAnti-Nephrin antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab58968
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
Quantity50 UG
HostRabbit
Conjugate/TagUnconjugated
Formatab58968 is purified
Concentration0.2 mg/ml
Gene NameNPHS1
ReactivityHuman, Mouse
TargetNephrin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) tRF-003634 alleviates adriamycin-induced podocyte injury by reducing the stability of TLR4 mRNAPLOS ONEOctober 19, 2023Xiaoqing Gao, Yunyang Qiao, Shanwen Li, Huimin Shi, Gaoting Qu, Jialing Ji, Weihua Gan, Aiqing ZhangThe primary antibodies used were: GAPDH (1:1000, 2118, Cell Signaling, USA), anti-nephrin (1:1000, ab58968, Abcam, UK), anti-podocin (1:1000, ab50339, Abcam, UK), anti-toll-like receptor 4 (TLR4, 1:1000, AF7017,

(2) Nicotine exacerbates diabetic nephropathy through upregulation of Grem1 expressionMolecular MedicineJuly 6, 2023Jianning Chen, Haiting Xiao, Rui Xue, Vinod Kumar, Rukhsana Aslam, Syed Faizan Mehdi, Huairong Luo, Ashwani Malhotra, Xiqian Lan, Pravin Singhalrabbit anti-Grem1 (Novus Biologicals, NBP1-31150, 1:100), rabbit anti-Nephrin (Abcam, Cambridge, MA, ab58968, 1:100), rabbit anti-P-Smad2/3 (Abcam, ab272332, 1:100), rabbit anti-P-Smad1/5/8 (Sigma-Aldrich, Burlington,

(3) Acute and long-term effects of cannabinoids on hypertension and kidney injuryScientific ReportsDecember 1, 2022Daria Golosova, Vladislav Levchenko, Olha Kravtsova, Oleg Palygin, Alexander Staruschenkorenal cortex homogenates or urine samples were assessed using primary antibodies (nephrin, 1:1000, #ab58968, Abcam; β arrestin 1 1:1000, #A0998, Abclonal; phospho-β arrestin 1, 1:1000, # 2416, Cell Signaling;

(4) Polydatin Ameliorates High Fructose-Induced Podocyte Oxidative Stress via Suppressing HIF-1Î±/NOX4 PathwayPharmaceuticsOctober 16, 2022Hong Ding, Chuanfeng Tang, Wei Wang, Ying Pan, Ruiqing Jiao, Lingdong Kongantibodies were as follows: anti-synaptopodin (sc-515842, 1:100, Santa Cruz, Dallas, TX, USA), anti-nephrin (ab58968, 1:100, Abcam, Cambridge, MA, USA) and anti-podocin (ab50399, 1:100, Abcam, Cambridge, MA, USA). After

(5) CD36 aggravates podocyte injury by activating NLRP3 inflammasome and inhibiting autophagy in lupus nephritisCell death & diseaseAugust 23, 2022Lv F, He Y, Xu H, Li Y, Han L, Yan L, Lang H, Zhao Y, Zhao Z, Qi Y.Abcam4), rabbit anti-p62 (ab91526, Abcam), rabbit anti-NPHS2 (ab50339, Abcam), rabbit anti-nephrin (ab58968, Abcam), and rabbit anti-beta-Actin (20536-1-AP, Proteintech). The secondary antibody was a peroxidase-conjugated

(6) miR-188-3p abolishes germacrone-mediated podocyte protection in a mouse model of diabetic nephropathy in type I diabetes through triggering mitochondrial injuryBioengineeredJanuary 5, 2022Yunguang Wang, Fangfang Feng, Wenfang He, Lifang Sun, Qiang He, Juan Jin(Ab181602, Abcam, 1:1000), GPX4 (Ab125066, Abcam, 1:3000), Prodocin (Ab50399, Abcam, 1:1000), and nephrin (Ab58968, Abcam, 1:1000) overnight at 4°C. HRP-conjugated goat anti-rabbit or anti-mouse IgG (BA1054, BA1053,

(7) Emodin Ameliorates Renal Damage and Podocyte Injury in a Rat Model of Diabetic Nephropathy via Regulating AMPK/mTOR-Mediated Autophagy Signaling PathwayDiabetes, Metabolic Syndrome and Obesity: Targets and TherapyMarch 18, 2021Hong Liu, Quan Wang, Ge Shi, Wenqiang Yang, Yanmin Zhang, Weidong Chen, Sheng Wan, Fei Xiong, Zengsi WangWestern Blot Analysis Western blot analysis was performed using the following antibodies: anti-rat nephrin (ab58968), anti-rat AMPK (ab80039), anti-rat p-AMPK (ab23875), anti-rat β-actin (ab227387) antibodies were from

(8) Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer’s diseaseNeural Regeneration ResearchMarch 1, 2021Yan-Zhen Zhou, Gui-Qiong Liu, Yan-Zhen Zhang, Yuan-Jie Liu, Wei Zhang, Gui-Qiong Luo1:1000; Abcam), insulin-degrading enzyme (IDE; Cat# ab133561; 1:1000; Abcam), neprilysin (NEP; Cat# ab58968; 1:1000; Abcam), tau-5 (Cat# ab80579; 1:1000; Abcam), paired helical filament-1 (PHF-1; Cat# ab184951;

(9) Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injuryJCI insightFebruary 8, 2021Lee HJ, Mariappan MM, Norton L, Bakewell T, Feliers D, Oh SB, Donati A, Rubannelsonkumar CS, Venkatachalam MA, Harris SE, et al.(catalog 9271, Cell Signaling Technology), Akt (catalog 9272, Cell Signaling Technology), nephrin (catalog ab58968, Abcam), and actin (catalog A2066, MilliporeSigma). Commercial ELISA kits were used for measuring phospho-Tyr-1150/1151-IR

(10) Role of miRNA-671-5p in Mediating Wnt/β-Catenin-Triggered Podocyte InjuryFrontiers in pharmacologyJanuary 1, 2021Wang C, Liu J, Zhang X, Chen Q, Bai X, Hong X, Zhou L, Liu Y.were as follows: anti-podocalyxin (AF1556; R&D Systems), anti-ZO-1 (402200; Invitrogen), anti-nephrin (ab58968; Abcam), anti-WT1 (sc-393498; Santa Cruz Biotechnology), anti-fibronectin (F3648; Sigma), anti-α-SMA

(11) Empagliflozin alleviates podocytopathy and enhances glomerular nephrin expression in db/db diabetic miceWorld Journal of DiabetesDecember 15, 2020Vadim V Klimontov, Anton I Korbut, Iuliia S Taskaeva, Nataliya P Bgatova, Maksim V Dashkin, Nikolai B Orlov, Anna S Khotskina, Evgenii L Zavyalov, Thomas KleinFor IHC, formalin-fixed, paraffinembedded 5-µm sections were prepared. Primary antibody to nephrin (1:100, ab58968, Abcam, United Kingdom) or transforming growth factor beta (TGF-β) (1:100, ab66043, Abcam, United Kingdom)

(12) Role of opioid signaling in kidney damage during the development of salt-induced hypertensionLife science allianceDecember 1, 2020Golosova D, Palygin O, Bohovyk R, Klemens CA, Levchenko V, Spires DR, Isaeva E, El-Meanawy A, Staruschenko A.(caspase 3, 1:1,000, #PA5-77887; Thermo Fisher Scientific; TRPC6, 1:200, #ACC-017; Alomone; nephrin, 1:500, #ab58968; Abcam). The membranes were blocked with 2% BSA in TBS and 0.01% Tween 20 overnight at room temperature

(13) Plasminogen Deficiency and Amiloride Mitigate Angiotensin II–Induced Hypertension in Type 1 Diabetic Mice Suggesting Effects Through the Epithelial Sodium ChannelJournal of the American Heart AssociationDecember 1, 2020Henrik Andersen et al.actin antibody, 1:1000, ab5694, Abcam, Cambridge, UK; polyclonal rabbit anti-nephrin antibody, 1:1000, ab58968, Abcam; polyclonal rabbit anti-mouse plasminogen, 1:1000, ab154560, Abcam; polyclonal rabbit anti-pro-renin

(14) Huidouba Improved Podocyte Injury by Down-Regulating Nox4 Expression in Rats With Diabetic NephropathyFrontiers in PharmacologyNovember 30, 2020KunBao Yang, YingHui Bai, Ning Yu, BiNan Lu, GuiYan Han, ChangJiang Yin, ZongRan Pangrabbit monoclonal [UOTR1B493] to NADPH oxidase 4 (No. ab133303), and rabbit polyclonal to nephrin (No. ab58968) were purchased from Abcam Trading (Shanghai) Co., Ltd. The goat anti-rabbit IgG DyLight 488 (No. A23220)

(15) Lycopene attenuates high glucose‑mediated apoptosis in MPC5 podocytes by promoting autophagy via the PI3K/AKT signaling pathwayExperimental and Therapeutic MedicineJuly 13, 2020Qingfen Wang, Rui Li, Zhi Xiao, Cun Houincubated at 4˚C overnight with the following primary antibodies: Anti-nephrin (rabbit; 1:400; cat. no. ab58968; Abcam), anti-podocin (rabbit; 1:1,300; cat. no. ab50339; Abcam), anti-Bcl-2 (rabbit; 1:1,000; cat.

(16) EDA2R mediates podocyte injury in high glucose milieuBiochimieJuly 1, 2020Xiqian Lan et al.in our previous publications [21–23]. Primary antibodies for EDA2R (ab203667, 1:1000) and nephrin (ab58968, 1:1000) were purchased from Abcam (Cambridge, MA), and antibodies for Bax (sc-526, 1:1000), Bcl2 (sc-492-g,

(17) Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stressCell death & diseaseMay 5, 2020Tian Y, Guo H, Miao X, Xu J, Yang R, Zhao L, Liu J, Yang L, Gao F, Zhang W, et al.overnight at 4 °C with a 1:1000 dilution of nestin (Abcam, #ab11306, RRID: AB_1640723), nephrin (Abcam, #ab58968, RRID: AB_944400), p-nephrin-Y1217 (Abcam, #ab80298, RRID: AB_1603375), PINK1 (Proteintech, 23274-1-AP),

(18) MiR193a Modulation and Podocyte PhenotypeCellsApril 17, 2020Jha A, Saha S, Ayasolla K, Vashistha H, Malhotra A, Skorecki K, Singhal PC.Rosemont, IL, USA), WT1 (rabbit polyclonal, 1:1000, Abcam, Cambridge, MA, USA), nephrin (rabbit polyclonal #ab58968; Abcam, Cambridge, MA, USA), CD2AP (rabbit polyclonal; #SC 25272; Santa Cruz Biotechnology, Dallas,

(19) Altered Expression of Long Noncoding and Messenger RNAs in Diabetic Nephropathy following Treatment with RosiglitazoneBioMed Research InternationalJanuary 14, 2020Liwen Zhang, Ying Zhou, Fangfang Zhou, Xialian Yu, Jian Liu, Yunzi Liu, Yufei Zhu, Weiming Wang, Nan Chenno. sc-8422; 1 : 300; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit anti-nephrin (cat. no. ab58968; 1 : 300; Abcam, Cambridge, MA, USA), mouse anti-E-cadherin (cat. no. ab76055; 1 : 1 000; Abcam), rabbit

(20) The Therapeutic Effect of Active Vitamin D Supplementation in Preventing the Progression of Diabetic Nephropathy in a Diabetic Mouse ModelJournal of diabetes researchJanuary 1, 2020Nakhoul N, Thawko T, Farber E, Dahan I, Tadmor H, Nakhoul R, Hanut A, Salameh G, Shagrawy I, Nakhoul F.Novus Biologicals), anti-villin (1 : 1000, LSC407669, Lifespan Biosciences), anti-nephrin (1 : 400, ab58968, Abcam), anti-podocin (1 : 2000, EPR 13820, Abcam), and anti-fibronectin (1 : 2000, Abcam). Secondary

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12/22/2015Western Blot Using Anti-Nephrin Antibody from Abcam Andreas EisenreichWe performed Western blot analyses of nephrin protein expression in cell lysates of human podocytes using the anti-Nephrin antibody from Abcam. We detected nephrin.

Anti-Nephrin antibody from Abcam

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Anti-Nephrin antibody from Abcam

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