YAP1 Antibody - BSA Free NB110-58358 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemYAP1 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNB110-58358
ImmunogenThis YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937]
Concentration1.0 mg/ml
ReactivityCanine,Human,Mouse,Rat,Zebrafish,Fish - Danio rerio (Zebrafish)
TargetYAP1
Quantity0.1 ml (also 0.025 ml)
FormatPBS
ApplicationsChemotaxis,IF/IHC,IHC-F,Chromatin Immunoprecipitation,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunoblotting,Simple Western,Chromatin Immunoprecipitation (ChIP), Knockdown Validated,Immunocytochemistry/ Immunofluorescence
ValidationKnockout Validated
Antibody TypePrimary Antibodies
IsotypeIgG
Research AreaBreast Cancer, Cancer, Chromatin Research, Core ESC Like Genes, Signal Transduction, Stem Cell Markers, Transcription Factors and Regulators, Tyrosine Kinases
Molecular Weight48 kDa
PurityImmunogen affinity purified
ClonalityPolyclonal
NCBI Full Gene NameYes1 associated transcriptional regulator
HostRabbit
Conjugate/TagUnconjugated
NCBI Gene AliasesYAP-1, YAP65, COB1, YKI, YAP, YAP2
StorageAliquot and store at -20C or -80C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)

Sizes Available: 0.1 ml (also 0.025 ml)

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Questions and Answers

Can this YAP1 antibody be used for ChIP assays, and if so, how has it been utilized in research?

Yes, the YAP1 antibody has been used in chromatin immunoprecipitation (ChIP) assays. For instance, in a study by Cai et al., the antibody was employed to investigate the binding of YAP to c-Myc's promoter, demonstrating its role in the regulation of gene expression related to polycystic kidney disease ().

What species can this YAP1 antibody react with, and how has it been validated in different models?

The YAP1 antibody is reactive with several species, including human, mouse, rat, canine, and zebrafish. It has been validated in various applications, including immunohistochemistry and immunofluorescence, as shown in studies involving mouse models of glioblastoma and kidney disease, where it effectively detected YAP1 expression (, ).

Has this antibody been cited in studies related to cancer, and what specific roles has YAP1 been shown to play?

Yes, the YAP1 antibody has been cited in numerous studies related to cancer. For example, in glioblastoma research, YAP1 was found to be activated in PTEN-deficient models, promoting macrophage infiltration and tumor progression (). Additionally, YAP1 has been implicated in the regulation of cellular senescence and proliferation in chondrosarcoma, highlighting its oncogenic potential ().

What are some specific applications of this YAP1 antibody in experimental protocols?

The YAP1 antibody has been utilized in various experimental protocols, including Western blotting, immunocytochemistry, and immunoprecipitation. It has been particularly effective in studies examining the Hippo signaling pathway and its implications in diseases such as heart failure and kidney disease, where it plays a critical role in cellular responses and survival mechanisms (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) YAP Promotes Microtubule Growth to Facilitate Sarcomere Disassembly in Adult CardiomyocytesCirculationAugust 11, 2026Feng Zhang, Jenna K. Cusick, Jie Liang, Fangfei Wang, Andrew T. Fernandes, Jacob T. Gafranek, Joshua S. Waxman, Zhiyuan Chen, Katherine E. Yutzey, Shijie Liuand 1× EDTA-free protease inhibitor cocktail [Roche]) with rabbit anti-YAP antibody (1:100; Novus, NB110–58358) or mouse anti-YAP antibody (1:100; Santa Cruz, sc-101199). After washing away unbound antibody, 500

(2) ERK‐Mediated Phosphorylation of YAP Defines a Noncanonical FGF Signaling Mechanism in Stem CellsAdvanced ScienceApril 10, 2026Xiaolei Zhao, Shannon Erhardt, Li Tang, Xiaotong Chen, Stephen M Farmer, Zixiu Cheng, Wen Chen, Ella Ziyuan Lu, Kihan Sung, Chang‐Ru Tsai, et al.manufacturer's guidelines. The nuclear extracts were then incubated with anti‐Yap antibody (Novus, NB110‐58358) or anti‐rabbit‐IgG antibody (Millipore Sigma, 12370) overnight at 4°C. For 293T cells, 0.5% NP‐40 lysis

(3) Hippo signaling differentially regulates distal progenitor subpopulations and their transitional states to construct the mammalian lungsNature CommunicationsApril 3, 2026Kuan Zhang, Madhuri Basak, Youssef Zaher, Erica Yao, Shao-An Wang, Thin Aung, Pao-Tien ChuangSanta Cruz Biotechnology, Cat# sc-101199; RRID:AB_1131430), rabbit anti-YAP1 (1:100, Novus Biologicals, Cat# NB110-58358; RRID:AB_1849483), goat anti-CTGF (1:100; Santa Cruz Biotechnology #sc-14939; RRID:AB_638805). Secondary

(4) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJournal of the American Heart AssociationMarch 17, 2026Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.Calbiochem, #400060) 1:100; anti‐CD31 (goat, bio‐techne, #AF3628) 1:100; anti‐YAP1 (rabbit, Novus, #NB110‐58358) 1:100; anti‐Ly6G (rat, BD Biosciences, #551459) 1:100; and anti‐CD3 (rat, Bio‐RAD, #MCA1477T) 1:100.

(5) Cell jamming transition is regulated by mitochondrial pyruvate transport and endocytosisbioRxivFebruary 13, 2026Alexandra Bermudez, Zoe Latham, Johnny Diaz, Weihong Yan, Jerry Chen, Dapeng Bi, Andrew Goldstein, Jimmy K Hu, Neil Y.C. Linminutes with the following primary antibodies: E-cadherin (Abcam, ab11512), YAP (Novus Biologicals, NB110-58358), RhoA (Cell Signaling Technology, 2117T), Rac-1 GTP (NewEast Biosciences, 26903), or EEA1 (BD Biosciences,

(6) Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial EnvironmentJ Am Heart AssocNovember 26, 2025Ria Göttert, Majed Kikhia, Marie‐Louise Herzog, Wen Pan, Alexandra Klaus‐Bergmann, January Weiner, Dieter Beule, Samuel Knauss, Golo Kronenberg, Michael Potente, et al.Calbiochem, #400060) 1:100; anti‐CD31 (goat, bio‐techne, #AF3628) 1:100; anti‐YAP1 (rabbit, Novus, #NB110‐58358) 1:100; anti‐Ly6G (rat, BD Biosciences, #551459) 1:100; and anti‐CD3 (rat, Bio‐RAD, #MCA1477T) 1:100.

(7) H3K79 methylation and H3K36 tri-methylation synergistically regulate gene expression in pluripotent stem cellsBIORXIVMay 13, 2025Emmalee W. Cooke, Cheng Zeng, Suza Mohammad Nur, Yunbo Jia, Aileen Huang, Jiwei Chen, Peidong Gao, Fei Xavier Chen, Fulai Jin, Kaixiang Cao(Cell Signaling Technology 14958), anti-RNA Pol II phospho-CTD Ser-5 (Millipore 04-1572), anti-YAP1 (Novus Biologicals NB110-58358), anti-phosphorylated YAP (Cell Signaling Technology 4911), and anti-TEAD4 (Santa Cruz sc-101184). The

(8) Microtubules Sequester Acetylated YAP in the Cytoplasm and Inhibit Heart RegenerationCirculationJanuary 7, 2025Shijie Liu, Vaibhav Deshmukh, Fansen Meng, Yidan Wang, Yuka Morikawa, Jeffrey D. Steimle, Rich Gang Li, Jun Wang, James F. Martinthose for the paraffin-embedded sections. Antibodies used were as follows: rabbit anti-YAP (1:200, NB110–58358, Novus Biologicals, Littleton, CO), mouse anti-YAP (1:200, sc-101199, Santa Cruz), mouse anti-troponin T

(9) Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axonsCurrent biology : CBOctober 7, 2024Sousa SC, Aroso M, Bessa R, Veríssimo E, Ferreira da Silva T, Lopes CDF, Brites P, Vieira J, Vieira CP, Aguiar PC, et al.

(10) Microtubules Sequester Acetylated YAP in the Cytoplasm and Inhibit Heart RegenerationCirculationAugust 26, 2024Shijie Liu, Vaibhav Deshmukh, Fansen Meng, Yidan Wang, Yuka Morikawa, Jeffery D. Steimle, Rich Gang Li, Jun Wang, James F. Martinthose for the paraffin-embedded sections. Antibodies used were as follows: rabbit anti-YAP (1:200, NB110–58358, Novus Biologicals, Littleton, CO), mouse anti-YAP (1:200, sc-101199, Santa Cruz), mouse anti-troponin T

(11) Blastocyst-like Structures in the Peripheral Retina of Young Adult BeaglesInternational Journal of Molecular SciencesMay 30, 2024Tsunehiko Ikeda, Denan Jin, Shinji Takai, Kimitoshi Nakamura, Emika Nemoto, Shota Kojima, Hidehiro Oku(which is present inside the nucleus in the trophectoderm [58]). Rabbit anti-YAP1 polyclonal antibody (NB110-58358; Novus Biologicals, LLC, Centennial, CO, USA) was used as the primary antibody at a 1:100 dilution after

(12) YAP1 maintains active chromatin state in head and neck squamous cell carcinomas that promotes tumorigenesis through cooperation with BRD4Cell reportsJune 14, 2022Chen N, Golczer G, Ghose S, Lin B, Langenbucher A, Webb J, Bhanot H, Abt NB, Lin D, Varvares M, et al.

(13) Metformin and LW6 impairs pancreatic cancer cells and reduces nuclear localization of YAP1Journal of CancerJanuary 1, 2020Zhang X, Liu P, Shang Y, Kerndl H, Kumstel S, Gong P, Vollmar B, Zechner D.Then these cells were incubated with YAP1-antibody (Novus Biologicals, Littleton, Colorado, USA, code NB110-58358) for 1.5 hours at room temperature, followed by the second antibody, Alexa Fluor 488 (Sigma-Aldrich,

(14) Podocyte-Specific Deletion of Yes-Associated Protein Causes FSGS and Progressive Renal FailureJournal of the American Society of NephrologyJanuary 1, 2016Monica Schwartzman, Antoine Reginensi, Jenny S. Wong, John M. Basgen, Kristin Meliambro, Susanne B. Nicholas, Vivette D'Agati, Helen McNeill, Kirk N. Campbellthis study were as follows: mouse anti-synaptopodin (courtesy Dr. P. Mundel), Rabbit anti-YAP (Novus Nb11058358), cleaved poly adenosine diphosphate ribose polymerase p25 (Abcam, Inc.), cleaved caspase 3 (Abcam, Inc.),

(15) Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAPJournal of cellular biochemistryJanuary 1, 0001"Miao HL, Pan ZJ, Lei CJ, Wen JY, Li MY, Liu ZK, Qiu ZD, Lin MZ, Chen NP, Chen M."

(16) Yap1 activation enables bypass of oncogenic Kras addiction in pancreatic cancerCellJanuary 1, 0001"Kapoor A, Yao W, Ying H, Hua S, Liewen A, Wang Q, Zhong Y, Wu CJ, Sadanandam A, Hu B, et al."

(17) Matrix-Stiffness-Regulated Inverse Expression of Krüppel-Like Factor 5 and Krüppel-Like Factor 4 in the Pathogenesis of Renal FibrosisThe American journal of pathologyJanuary 1, 0001"Chen WC, Lin HH, Tang MJ."

(18) Flow-Dependent Endothelial YAP Regulation Contributes to Vessel MaintenanceDevelopmental cellJanuary 1, 0001"Nakajima H, Yamamoto K, Agarwala S, Terai K, Fukui H, Fukuhara S, Ando K, Miyazaki T, Yokota Y, Schmelzer E, et al."

(19) Selective inhibition of stromal mechanosensing suppresses cardiac fibrosisNatureJanuary 1, 0001"Cho S, Rhee S, Madl CM, Caudal A, Thomas D, Kim H, Kojic A, Shin HS, Mahajan A, Jahng JW, et al."antibodies in 0.5% BSA solution, with gentle agitation at 4°C: αSMA (1:200, #ab7817, Abcam), YAP1 (1:500, #NB110-58358, Novus Biologicals), Ki67 (1:2000, #ab15580, Abcam), MRTF-A/MKL1 (1:150, #NBP2-45862, Novus Biologicals),

(20) RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cellsOncotargetJanuary 1, 0001"Brusgard JL, Choe M, Chumsri S, Renoud K, MacKerell AD Jr, Sudol M, Passaniti A."#2578), β-actin (Sigma/Aldrich), GAPDH (Cell Signaling, 14C10, #2118), and YAP1 (Novus Biologicals, NB110-58358). Protein levels were normalized to actin and quantified using NIH Image-J software. Immunoprecipitation/co-immunoprecipitation assay

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