ROCK1 (C8F7) Rabbit mAb 4035S from Cell Signaling Technology

Product Specs
ItemROCK1 (C8F7) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4035S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk
TargetROCK1
HostRabbit
NCBI Gene AliasesP160ROCK, ROCK-I
ClonalityMonoclonal
NCBI Full Gene NameRho associated coiled-coil containing protein kinase 1
Research AreaCytoskeletal Signaling
CloneC8F7
Molecular Weight160

Questions and Answers

Can this antibody be used to study the role of ROCK1 in cancer cell migration and invasion?

Yes, the ROCK1 (C8F7) Rabbit mAb has been utilized in studies investigating its role in cancer cell migration and invasion. For instance, ROCK1 activation has been linked to cytoskeleton remodeling in cervical cancer cells, enhancing their metastatic potential through the RhoA/ROCK signaling pathway, as demonstrated in research by Mao et al. (2023).

Is this antibody effective for detecting ROCK1 in Western blotting?

The ROCK1 (C8F7) Rabbit mAb has been shown to be effective for Western blotting applications, as evidenced by its use in various studies, including those that assess ROCK1 expression levels in different cell types and conditions, such as in the context of hypoxia-induced cell migration in ovarian cancer (Tang et al., 2015).

What species can this antibody detect, and is it suitable for use in human samples?

The ROCK1 (C8F7) Rabbit mAb is reactive with multiple species, including humans, mice, rats, and non-human primates. This broad reactivity makes it suitable for use in human samples, as well as in comparative studies across different model organisms.

Are there any specific signaling pathways associated with ROCK1 that this antibody can help elucidate?

Yes, the ROCK1 (C8F7) Rabbit mAb can help elucidate pathways involving RhoA and downstream signaling mechanisms. For example, studies have shown that ROCK1 is involved in mediating vascular dysfunction in diabetes through its activation by ErbB2 signaling, highlighting its role in various cellular processes related to cytoskeletal dynamics and cell migration (Akhtar et al., 2013).

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Rnd3 regulates lung cancer cell invasion and migration independently of ROCK1 signaling via alpha 5 integrin modulationLife Science AllianceMay 1, 2026Noemi Garcia Garcia, Thanh Ha Vy Nguyen, Dane Richey, Emily F Cox, Jon Coca Juaristi, Cody Ashby, Analiz Rodriguez, Katie R RyanPVDF membrane (Invitrogen). Primary antibodies used in this study were as follows: Rnd3 (3664), ROCK1 (4035), RhoA (2117) pMLC2 (3671), pCofilin (3313), Cofilin (5175), alpha 5 (4705), alpha V (4711), beta 1

(2) Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivationPLoS OneApril 17, 2026Aiyuan Cai, Zilong Li, Ran Liu, Hailong Huang, Ping Liu, Ruizhong Zhang, Jing Xiao, Yuanhong Lin, Qingpeng Hu, Haixia Wu(Cell Signaling Technology, USA, 2117, dilution ratio 1:1000), ROCK antibody (Cell Signaling Technology, USA, 4035, dilution ratio 1:1000), and GAPDH (Cell Signaling Technology, USA, 5174, dilution ratio 1:1000). The

(3) ROCK1 inhibition primes anti-tumor immunity in EGFR-mutant NSCLC by triggering PD-L1 degradation mediated by SMURF2Molecular CancerApril 2, 2026Menglin Bai, Qianqian Gao, Peng Jin, Lin Ma, Ji Li, Leilei Wu, Weiqing Wang, Luojia Lv, Yintao Li, Bo Cheng, et al.Biolegend), BV785 anti-mouse CD206 (141729, Biolegend), BV711 anti-mouse CD11c (117349, Biolegend), ROCK1 (4035, Cell Signaling Technology), PD-L1 (13684, Cell Signaling Technology), PD-L1 (66248, Proteintech), β-actin

(4) Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signalingMaterials Today BioApril 1, 2026Ji Hoon Jeong, Sung Sik Hur, Han Gyu Cha, Min-Kyu Kim, Ali Taghizadeh, Yong Hwee Tan, Le Anh Nguyet, Gahyun Kim, Yu Suk Choi, Hae-Won Kim, et al.Thermo Fisher Scientific, Waltham, MA, USA), GAPDH (MA515738, Thermo Fisher Scientific, Waltham, MA, USA), ROCK1 (4035S, Cell Signaling, Massachusetts, MA, USA), ROCK2 (9029S, Cell Signaling, Massachusetts, MA, USA), fibronectin

(5) Macrophage Migration Inhibitory Factor (MIF)-CD74 Signaling Pathway Mediates Trabecular Meshwork Dysfunction in GlaucomabioRxivMarch 24, 2026Monu Monu, Lal Krishan Kumar, Prince Kumar, Gulab Zode, Pawan Kumar SinghpSTAT3 (#9145), STAT3 (#9139), RIP3K (#10188), pMLKL (#18640), MLKL (#14993), RhoA (#2117), ROCK1 (#4035), pMLC (#3674), MLC (#3672), MIF (#75038), CD74 (#77274), Blimp-1 (#9115) (Cell Signaling Technology,

(6) Unveiling critical signaling pathways in the murine salivary gland and the role of midkineiScienceMarch 20, 2026Theresa Wrynn, Jason Osinski, Satrajit Sinha, Rose-Anne RomanoStudiesHybridoma Bank,http://dshb.biology.uiowa.edu/; RRID:AB_2133570 Rabbit anti-Rock1,Cell Signaling,4035; RRID:AB_2238679 Sheep anti-Midkine,R&D Systems,af7769; RRID:AB_2917965 Rabbit anti-Midkine,ProteinTech,11009-1-AP;

(7) Extracellular Vesicles Coordinate Bacterial Cloaking in Lung Epithelial Cells to Alleviate Acute Inflammatory InjuryJ Extracell VesiclesFebruary 8, 2026Feng Ding, Shengkai Gong, Haotian Luo, Dandan Wu, Xiaoshan Yang, Zihan Li, Dingmei Zhang, Peijie He, Jiani Liu, Lili Bao, et al.(ab268020) from Abcam; anti‐Fibrinogen γ (D6) (sc‐133226) from Santa Cruz; anti‐Alix (92880), anti‐ROCK1 (4035), anti‐Phospho‐FAK (Tyr397) (3283s), and anti‐Rab27a (69295s) from Cell Signalling Technology; anti‐CD9

(8) Oxypeucedanin attenuates neuropathic pain in male mice via inhibiting lysophosphatidic acid receptor signalingiScienceJanuary 16, 2026Jiejia Li, Wenting Tang, Liyun Wang, Yipeng Gu, Wenhui You, Wenxin Yang, Yanlong Huang, Xinyue Yu, Ruiyang Zhu, Yue Xu, et al.cord sections were incubated overnight at 4 °C with rabbit anti-ROCK1(Cell Signaling Technology Cat# 4035, RRID:AB_2238679), rabbit anti-RhoA((Abcam Cat# ab187027, RRID:AB_2827434), then incubated with FITC-conjugated

(9) Therapeutic modulation of ROCK overcomes metabolic adaptation of cancer cells to OXPHOS inhibition and drives synergistic anti-tumor activitybioRxivJanuary 16, 2026Nicholas Blazanin, Xiaobing Liang, Iqbal Mahmud, Eiru Kim, Sara Martinez, Lin Tan, Waikin Chan, Nazanin Esmaeili Anvar, Min Jin Ha, Md Qudratullah, et al.Phenformin(Cat# S2542) were all purchased from Selleck Chemicals (Houston, TX). Antibodies against ROCK1 (Cat# 4035S), ROCK2 (Cat# 9029S), p-AMPK T172(Cat# 50081S), and AMPK (Cat# 2532S) were from Cell Signaling (Danvers,

(10) Loss of p190A RhoGAP induces aneuploidy and enhances bladder cancer cell migration and invasion by modulating actin dynamicsScientific ReportsNovember 18, 2025Qianyu Kang, Xue Kong, Gregoire Najjar, Anca Azoitei, Markus Eckstein, Axel John, Friedemann Zengerling, Felix Wezel, Christian Bolenz, Cagatay GünesChemie GmbH, Steinheim), Anti-RhoA (1:1000, sc-418, Santa Cruz Biotechnology, Inc), Anti-ROCK1 (1:1000, #4035, Cell Signaling Technology, lnc, USA), Anti-LIMK-1 (1:500, sc-28370, Santa Cruz Biotechnology, Inc),

(11) Immunomodulatory properties of mesenchymal stem cells within three-dimensional collagen matricesIn Vitro Cellular & Developmental Biology - AnimalOctober 1, 2025Yenny Yustisia, Koichi KatoCell Signaling Technology, Danvers, MA), Rho-associated coiled-coil containing protein kinase 1 (ROCK1; C8F7, #4035, Cell Signaling Technology), and β-actin (D6A8, #8457, Cell Signaling Technology). After washing, membranes

(12) Lipoprotein(a)/CD36 Interaction Drives IL-6/RhoA-GTP Signaling and miRNA Epigenetic Regulation in Coronary Artery SpasmPharmaceuticals (Basel)September 16, 2025Yen-Kuang Lin, Tsung-Han Hsieh, Chi-Tai Yeh, Vijesh Kumar Yadav, Iat-Hang Fong, Kuang-Tai Kuo, Nicholas G. Kounis, Patrick Hu, Ming-Yow Hung#ab9484, Lot #GAP2203), RhoA (Cell Signaling Technology, Cat #2117), RhoA-GTP (Cat #8820), ROCK1 (Cat #4035), ROCK2 (Cat #9029), t-MBS (Cat #2634), and p-MBS (Cat #3040). Protein bands were detected using the

(13) MicroRNA-129-5p-mediated translational repression of microglial ROCK1 leads to enhanced phagocytosisJournal of Biological ChemistryJuly 1, 2025Rajib Kumar Dey, Ranjana Kumari, Roni Patra, Dharmendra Kumar Soni, Roopa Biswas, Satyakam Patnaik, Debabrata Ghoshantibody for Western blot analysis & immunohistochemistry were procured from Cell signaling technology (4035S, dilution 1:2500) and G-biosciences (ITT4162–100u, dilution 1:1000) respectively. Beta Actin (sc-47778),

(14) SMARCA4 Inhibits Breast Cancer Progression and Metastasis through RHOA SuppressionCancer ResearchMay 15, 2025Zheng Sun, Zhuo Li, Yong Wei, Lillian Xu, Xiang Hang, Yibin Kanganti-RHOA (Cytoskeleton Cat# ARH05, RRID:AB_2884965, 1:500), anti-ROCK1 (Cell Signaling Technology Cat# 4035, RRID:AB_2238679, 1:1000), anti-ROCK2 (Cell Signaling Technology Cat# 9029, RRID:AB_11127802, 1:1000),

(15) SMARCA4 Inhibits Breast Cancer Progression and Metastasis through RHOA SuppressionCancer ResMay 15, 2025Zheng Sun, Zhuo Li, Yong Wei, Lillian Xu, Xiang Hang, Yibin Kanganti-RHOA (Cytoskeleton Cat# ARH05, RRID:AB_2884965, 1:500), anti-ROCK1 (Cell Signaling Technology Cat# 4035, RRID:AB_2238679, 1:1000), anti-ROCK2 (Cell Signaling Technology Cat# 9029, RRID:AB_11127802, 1:1000),

(16) Drug and siRNA screens identify ROCK2 as a therapeutic target for ciliopathiesCommun Med (Lond)April 19, 2025Claire E. L. Smith, Andrew J. Streets, Alice V. R. Lake, Subaashini Natarajan, Sunayna K. Best, Katarzyna Szymanska, Magdalena Karwatka, Thomas Stevenson, Rachel Trowbridge, Gary Grant, et al.(CST 3671); phosphorylated myosin light chain II (Thr18/Ser19) (CST 3674); anti-ROCK1 (clone C8F7, CST 4035); anti-ROCK2 (Bethyl A300-047A); anti-IFT88 (Proteintech 13967-1-AP), anti-GAPDH (CST 2118); anti-β-actin

(17) Noncanonical Wnt/Ror2 Signaling Regulates Basal Cell Fidelity and Branching Morphogenesis in the Mammary GlandbioRxivFebruary 28, 2025Hongjiang Si, Erika Mendoza Mendoza Mendoza, Madelyn Esquivel, Chad J. Creighton, Jianming Xu, Kevin RoartyIowa City, IA), RhoA (1:1000; #2117; Cell Signaling Technology [CST], Danvers, MA), ROCK1 (1:1000; #4035; CST), Cdc42 (1:1000; #2466; CST), Rac1/2/3 (1:1000; #2465; CST), YAP/TAZ (1:1000; #8418; CST), phosphorylated

(18) Activation of S1PR2 on macrophages and the hepatocyte S1PR2/RhoA/ROCK1/MLC2 pathway in vanishing bile duct syndromePLoS OneJanuary 24, 2025Aya Miyagawa-Hayashino, Tetsuya Imura, Toshiaki Takezawa, Maki Hirai, Saya Shibata, Hiroshi Ogi, Takahiro Tsujikawa, Eiichi Konishithe manufacturer’s instructions. Antibodies against RhoA (sc-418, Clone 26C4, Santa Cruz), ROCK1 (#4035, Clone C8F7, Cell Signaling), S1PR2 (Proteintech), and Gapdh (ab9485, Abcam) were used for the analysis. Enzyme-linked immunosorbent assay

(19) AKR1B1-dependent fructose metabolism enhances malignancy of cancer cellsCell Death & DifferentiationDecember 1, 2024Qing Zhao, Bing Han, Lu Wang, Jia Wu, Siliang Wang, Zhenxing Ren, Shouli Wang, Haining Yang, Michele Carbone, Changsheng Dong, et al.(1:1,000, CST, 3195 T), anti-RhoA antibody (1:1,000, CST, 22117 T), anti-ROCK1 antibody (1:1,000, CST, 4035 T), anti-ROCK2 antibody (1:1,000, Abcam, ab125025), anti-Vinculin antibody (1:1,000, Abcam, ab91459),

(20) The time-dependent changes in a mouse model of traumatic brain injury with motor dysfunctionPLOS ONESeptember 6, 2024Dohee Kim, Jinsu Hwang, Jin Yoo, Jiyun Choi, Mahesh Ramalingam, Seongryul Kim, Hyong-Ho Cho, Byeong C. Kim, Han-Seong Jeong, Sujeong Jangsignaling,CST#9665,1:3000,WB Cleaved-caspase 3,Cell signaling,CST#9664,1:1000,WB ROCK 1,Cell signaling,CST#4035,1:1000,WB p53,Santa cruz,SC-126,1:500,WB β-actin,Cell signaling,CST#5125,1:3000,WB Biotinylated horse

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ROCK1 (C8F7) Rabbit mAb

ROCK1 (C8F7) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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ROCK1 (C8F7) Rabbit mAb from Cell Signaling Technology

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