Fig 2: Gas6-induced MerTK phosphorylation. ARPE-19 cells were cultured in standard medium (CTR) or high glucose (HG) for 24 h and then exposed to GAS6 for different times. (A) Representative Western blot. (B) Quantification of densitometries of relative bands. Data are expressed as mean ± SD of fold induction relative to β-actin of three independent experiments (n = 3). * p < 0.05 and ** p < 0.01 vs. CTR.

Fig 3: Expression of MerTK in ARPE-19 cells cultured in standard medium (CTR) or high glucose (HG) for 24 h. (A) MerTK gene expression normalized vs. housekeeping genes. (B) Representative Western blot analysis of MerTK protein expression and (C) quantification of densitometries of relative bands. Data are expressed as mean ± SD of fold induction relative to β-actin of three independent experiments (n = 3). ** p < 0.01 vs. CTR.

Fig 4: Detection of the extracellular region of MerTK in ARPE-19 cells cultured in standard medium (CTR) or high glucose (HG) for 24 h. (A) Representative Western blot image and (B) quantification of densitometries of relative bands. Data are expressed as mean ± SD of fold induction relative to β-actin of three independent experiments (n = 3). ** p < 0.01 vs. CTR.