PRAS40 Antibody 2610S from Cell Signaling Technology

Product Specs
ItemPRAS40 Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2610S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetPRAS40
HostRabbit
NCBI Gene AliasesLobe, PRAS40
NCBI Full Gene NameAKT1 substrate 1
ClonalityPolyclonal
Research AreaPI3K / Akt Signaling
Molecular Weight40

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) AKT-mediated phosphorylation of TSC2 controls stimulus- and tissue-specific mTORC1 signaling and organ growthDevelopmental CellOctober 6, 2025Yann Cormerais, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cissé, Jong Shin, Benjamin Stefadu, Jean Personnaz, Sandra Schrötter, Jessica Freed, Angelica D’Amore, et al.(CST, 9644), P-TSC2 T1462 (CST, 3617), ERK1/2 (CST, 9102) P-ERK1/2 T202/Y204 (CST, 4370), PRAS40 (CST, 2610), P-PRAS40 T246 (CST, 2997), mTOR (CST, 2983), P-mTOR S2448 (CST, 2971), AMPKα (CST, 2603), P-AMPKα

(2) Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infectionsImmunitySeptember 9, 2025Marco Di Gioia, Valentina Poli, Piao J. Tan, Roberto Spreafico, Anne Chu, Alex G. Cuenca, Zhongyang Wu, Mehdi Benamar, Laura Pandolfi, Federica Meloni, et al.,Cell Signaling Technology,Cat# 13175; RRID: AB_2798140 "Anti-PRAS40 Antibody, Unconjugated",Cell Signaling Technology,Cat# 2610; RRID: AB_916206 Phospho-GSK-3 (Ser9) (D85E12) XP® Rabbit mAb,Cell Signaling Technology,Cat# 5558;

(3) The interplay between FOXO3 and FOXM1 influences sensitivity to AKT inhibition in PIK3CA and PIK3CA/PTEN altered estrogen receptor positive breast cancerNPJ Breast CancerApril 22, 2025Valentina Cutano, Ming Li Chia, Eleanor M. Wigmore, Lorna Hopcroft, Stuart C. Williamson, Amanda L. Christie, Brandon Willis, James Kerr, Jenny Ashforth, Rhys Fox, et al.#9101S,1:400 pAKT S473 CST #4060,1:1000 AKT CST #9272,1:1000 pPRAS40 CST #2997,1:1000 PRAS40 CST #2610,1:1000 pS6 CST #2211,1:1000 S6 CST #2217,1:1000 PTEN CST #9559,1:1000 Vinculin CST #13901,1:1000 βactin

(4) Chronic UCN2 treatment desensitizes CRHR2 and improves insulin sensitivityNature CommunicationsJuly 4, 2023Stephen E. Flaherty, Olivier Bezy, Wei Zheng, Dong Yan, Xiangping Li, Srinath Jagarlapudi, Bina Albuquerque, Ryan M. Esquejo, Matthew Peloquin, Meriem Semache, et al.total AKT1S1, pSer3-IRS1, total IRS1, pSer473-AKT, and total AKT (Cell Signaling, Danvers, MA #5936, #2610, #2385, #2382, #4060, and #4691, respectively) diluted 1:1000. Following three washing steps with TBST

(5) PRMT5 promotes ovarian cancer growth through enhancing Warburg effect by methylating ENO1MedComm (2020)April 1, 2023Fei Xie, Han Zhang, Kongkai Zhu, Cheng‐Shi Jiang, Xiaoya Zhang, Hongkai Chang, Yaya Qiao, Mingming Sun, Jiyan Wang, Mukuo Wang, et al.arginine methylation residues on ENO1 protein (https://www.phosphosite.org/proteinAction.action?id = 2610&showAllSites = true and https://www.phosphosite.org/siteTableNewAction?id = 2610&showAllSites = true).

(6) P38 Mediates Tumor Suppression through Reduced Autophagy and Actin Cytoskeleton Changes in NRAS-Mutant MelanomaCancersJanuary 31, 2023Banik I, Ghosh A, Beebe E, Burja B, Frank Bertoncelj M, Dooley CM, Markkanen E, Dummer R, Busch-Nentwich EM, Levesque MP.,Cell Signaling,Phospho-pRAS40 13175,1:1000,Cell Signaling Phospho-mTORS2448 5536,1:1000,Cell Signaling,Total pRAS40 2610,1:1000,Cell Signaling Total mTOR 2983,1:1000,Cell Signaling,Phospho-PDK1 3061,1:1000,Cell Signaling Phospho-AKTS437

(7) Global-run on sequencing identifies Gm11967 as an Akt-dependent long noncoding RNA involved in insulin sensitivityiScienceJune 17, 2022Dominic Santoleri, Hee-Woong Lim, Matthew J. Emmett, Julian Stoute, Matthew J. Gavin, Jaimarie Sostre-ColÃ³n, Kahealani Uehara, Jaclyn E. Welles, Kathy Fange Liu, Mitchell A. Lazar, et al.,Cell Signaling Technology,Cat# 2997; RRID: AB_2258110 GCK,Gift from Magnuson Lab,N/A PRAS40,Cell Signaling Technology,Cat# 2610; RRID: AB_916206 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, AAV8-TBG-CRE,University

(8) Loss of FOXO transcription factors in the liver mitigates stress-induced hyperglycemiaMolecular MetabolismSeptember 1, 2021Anna E. Garcia Whitlock, Jamarie Sostre-Colón, Matthew Gavin, Niels D. Martin, Joseph A. Baur, Carrie A. Sims, Paul M. TitchenellCell Signaling Technology were used for immunoblotting: pAKT Ser473 (#9271), Akt (#2964), PRAS40 (#2610), pS6 (#2215), S6 (#2217), and HSP90 (#4874). IGFBP-1 (sc-6000) was from Santa Cruz. pPRAS40 (07–888)

(9) Hsp90-mediated regulation of DYRK3 couples stress granule disassembly and growth via mTORC1 signalingEMBO reportsMay 5, 2021Mediani L, Antoniani F, Galli V, Vinet J, Carrà AD, Bigi I, Tripathy V, Tiago T, Cimino M, Leo G, et al.,Cell Signaling,9205 Phospho‐ser10‐H3,Activemotif,39253 Phospho‐4E‐BP1 (Thr37/46),Cell Signaling,2855 p70 S6 Kinase,Cell Signaling,9202 PRAS40,Cell Signaling,2610 Puromycin (Clone 12D10),Merck,MABE343 SC‐35,Sigma‐Aldrich,S4015 TIA‐1,Santa Cruz Biotechnology,SC‐1751 TUBA4A,Sigma‐Aldrich,T6074 Ubiquitin,Dako,Z0458 4E‐BP1,Cell Signaling

(10) Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal CancerFrontiers in OncologyApril 8, 2021Xiaozhe Qian, Donglei Zhang, Ziang Cao, Haitao Ma(4H1) Mouse mAb (#9309), Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (#8516), PRAS40 Antibody (#2610), Phospho-PRAS40 (Thr246) (D4D2)Rabbit mAb (#13175), p-mTOR (#2971), and mTOR (#2983) and were purchased

(11) Dis3L2 regulates cell proliferation and tissue growth through a conserved mechanismPLoS geneticsDecember 1, 2020Towler BP, Pashler AL, Haime HJ, Przybyl KM, Viegas SC, Matos RG, Morley SJ, Arraiano CM, Newbury SF.9451S), anti-p4E-BP (T70) (Cell Signalling cat. no. 5078S) and anti-4E-BP (Cell Signalling cat. no. 2610S) were all used at 1:1000. Mouse anti-Tubulin primary antibody (Sigma, cat. no. T9026) was used at 1:2000

(12) Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived EicosanoidsCellJune 25, 2020Koundouros N, Karali E, Tripp A, Valle A, Inglese P, Perry NJS, Magee DJ, Anjomani Virmouni S, Elder GA, Tyson AL, et al.,Cell Signaling Technology,Cat# 4060; RRID: AB_2315049 Rabbit polyclonal anti-PRAS40,Cell Signaling Technology,Cat# 2610; RRID: AB_916206 Rabbit monoclonal anti-phospho-PRAS40 (Thr246),Cell Signaling Technology,Cat# 2997;

(13) AKT1 Regulates Endoplasmic Reticulum Stress and Mediates the Adaptive Response of Pancreatic β CellsMolecular and Cellular BiologyMay 14, 2020Zhechu Peng, Richa Aggarwal, Ni Zeng, Lina He, Eileen X. Stiles, Anketse Debebe, Jingyu Chen, Chien-Yu Chen, Bangyan L. Stiles9454; Cell Signaling), p-PRAS40 (Thr246) (number 2640; Cell Signaling Technologies), PRAS40 (number 2610; Cell Signaling Technologies), actin and vinculin (Sigma), and glyceraldehyde-3-phosphate dehydrogenase

(14) Integrin-mediated adhesions in regulation of cellular senescenceScience advancesMay 1, 2020Shin EY, Park JH, You ST, Lee CS, Won SY, Park JJ, Kim HB, Shim J, Soung NK, Lee OJ, et al.for immunoblotting/1:200 for immunohistochemistry), p53 (#2524; 1:1000), pp53 (S15) (#9284; 1:500), pPAK1 (T423) (#2610; 1:500), ppaxillin (Y118) (#2541; 1:500), and pγH2AX (S139) (#9713; 1:200) antibodies were purchased

(15) Lactic acidosis induces resistance to the pan-Akt inhibitor uprosertib in colon cancer cellsBritish Journal of CancerApril 28, 2020Emily M. E. Barnes, Yitao Xu, Adrian Benito, Lili Herendi, Alexandros P. Siskos, Eric O. Aboagye, Anke Nijhuis, Hector C. KeunAkt Serine (S) 473 (D9E, #4060), Phosphorylated PRAS40 Threonine (T) 246 (#2640) and total PRAS40 (#2610) purchased from Cell Signalling Technologies (Danvers, Massachusetts, US). Anti-β-actin (#A5441) was

(16) Targeting PI3Kβ alone and in combination with chemotherapy or immunotherapy in tumors with PTEN lossOncotargetMarch 17, 2020Nicci Owusu-Brackett, Ming Zhao, Argun Akcakanat, Kurt W. Evans, Erkan Yuca, Ecaterina Ileana Dumbrava, Filip Janku, Funda Meric-Bernstamanti-phospho-S6K (T389) (#9234), anti-S6K (#9202), anti-phospho-PRAS40(T246) (#2997), and PRAS40 (#2610). Anti-β-actin antibody (#A5441) was purchased from Sigma. Secondary antibodies Goat-anti-Rabbit-Alexa

(17) PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent MechanismCell reportsMay 14, 2019Wang DG, Paddock MN, Lundquist MR, Sun JY, Mashadova O, Amadiume S, Bumpus TW, Hodakoski C, Hopkins BD, Fine M, et al.RRID:AB_1147620 pPRAS40,Cell Signaling Technologies,Cat# 13175; RRID:AB_2798140 PRAS40,Cell Signaling Technologies,Cat# 2610; RRID:AB_916206 Beta-Actin,Abcam,Cat# Ab6276; RRID:AB_2223210 Tubulin,Abcam,Cat# Ab7291; RRID:AB_2241126 IRDye-secondary

(18) The PI3K and MAPK/p38 pathways control stress granule assembly in a hierarchical mannerLife science allianceApril 1, 2019Heberle AM, Razquin Navas P, Langelaar-Makkinje M, Kasack K, Sadik A, Faessler E, Hahn U, Marx-Stoelting P, Opitz CA, Sers C, et al.p70-S6K (Cat. No. 9202), PRAS40-pS183 (Cat. No. 5936), PRAS40-pT246 (Cat. No. 2997), PRAS40 (Cat. No. 2610), 4E-BP1-pT37/46 (Cat. No. 2855), 4E-BP1 (Cat. No. 9644), Akt-pT308 (Cat. No. 4056), Akt-pS473 (Cat.

(19) Gaudichaudione H Inhibits Inflammatory Responses in Macrophages and Dextran Sodium Sulfate-Induced Colitis in MiceFrontiers in pharmacologyJanuary 1, 2019Jiang Y, Xiao L, Fu W, Tang Y, Lertnimitphun P, Kim N, Zheng C, Tan H, Lu Y, Xu H.(#50081), AMPKα (#5831), phospho-proline-rich Akt substrate of 40 kDa (p-PRAS40) (#13175), PRAS40 (#2610), and β-actin (#4970) (Cell Signaling Technology, Danvers, MA, USA) and the antibody against TLR4 (sc-293072,

(20) TAPP Adaptors Control B Cell Metabolism by Modulating the Phosphatidylinositol 3-Kinase Signaling Pathway: A Novel Regulatory Circuit Preventing AutoimmunityJournal of immunology (Baltimore, Md. : 1950)July 15, 2018Jayachandran N, Mejia EM, Sheikholeslami K, Sher AA, Hou S, Hatch GM, Marshall AJ.(catalog no. 9271 and 9272; Cell Signaling Technology), phospho- and total PRAS40 (catalog no. 2640 and 2610; Cell Signaling Technology), and b-actin (catalog no. 4970; Cell Signaling Technology). Band intensity

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PRAS40 Antibody

PRAS40 Antibody from Cell Signaling Technology

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Cell Signaling Technology

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PRAS40 Antibody from Cell Signaling Technology

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