Fig 1: FMRP facilitates AGO binding in a large subset of brain mRNAs. (A) IP of FMRP treated with RNase A and probed for RPL5. Experiment performed in triplicate, representative immunoblot is shown. (B) Number of normalized peaks from AGO2 eCLIP in WT versus Fmr1 KO mouse brains. (C) Distribution of enriched AGO2 binding sites across transcript regions determined by eCLIP: WT mouse brain normalized to input (top), Fmr1 KO brains normalized to input (bottom). (D) Volcano plot of AGO2 enriched peaks in WT and Fmr1 KO mouse brain. (E) RT-qPCR of statistically differentially bound targets of AGO in the absence of FMRP (n = 3), error bars represent SD. (F) Immunoblot of protein levels of differentially bound AGO targets from WT and Fmr1 KO mouse brain. eIF5B is a loading control, (n = 3), error bars represent SD, *P < 0.05, ** P < 0.001 Student t-test.