Fig 2: Effect of RGS1 on the proliferation, migration, invasion, and apoptosis of osteosarcoma cells. (A) Validation of RGS1 high expression in MG63 and U2OS cells; (B) MG63 cell viability; (C) U2OS cell viability; (D) colony forming of MG63 and U2OS cells, which were stained with crystal violet; (E) wound healing of MG63 cells, images were magnified at ×100; (F) wound healing of U2OS cells, images were magnified at ×100; (G) invasive ability of MG63 and U2OS cells, which were stained with crystal violet, and images were magnified at ×200; (H) apoptotic rate of MG63 and U2OS cells; (I) validation of RGS1 silencing in MG63 and U2OS cells; (J) MG63 cell viability; (K) U2OS cell viability; (L) colony forming of MG63 and U2OS cells, which were stained with crystal violet; (M) wound healing of MG63 cells, images were magnified at ×100; (N) wound healing of U2OS cells, images were magnified at ×100; (O) invasive ability of MG63 and U2OS cells, which were stained with crystal violet, and images were magnified at ×200. (P) apoptotic rate of MG63 and U2OS cells. N=6, *P<0.05 versus Vector by t-test or one-way ANOVA. RGS1, regulator of G protein signaling 1; ANOVA, analysis of variance.

Fig 3: Effect of miR-376b-3p and RGS1 co-transfection on the proliferation, migration, invasion, and apoptosis of osteosarcoma cells. (A) Protein expression of RGS1; (B) viability of MG63 cells within 5 days; (C) viability of U2OS cells within 5 days; (D) colony forming of MG63 and U2OS cells, which were stained with crystal violet; (E) wound healing of MG63 cells, images were magnified at ×100; (F) wound healing of U2OS cells, images were magnified at 100×; (G) invasive ability of MG63 and U2OS cells, which were stained with crystal violet, and images were magnified at 200×; (H) apoptotic rate of MG63 and U2OS cells. N=6, *P<0.05 versus Vector by t-test or one-way ANOVA. RGS1, G protein signaling 1; ANOVA, analysis of variance.

Fig 4: Relationship between RGS1 and the clinical malignant phenotype of osteosarcoma. (A) RGS1 enrichment between high and low proliferation specimens; (B) RGS1 enrichment between high and low migration specimens; (C) RGS1 enrichment between high and low invasion specimens; (D) RGS1 levels in specimens with good or poor prognosis. RGS1, regulator of G protein signaling 1.

Fig 5: The targeting of RGS1 with miR-376b-3p. (A) Starbase predicted the interaction of RGS1 with miR-376b-3p and the mutant RGS1; (B) the targeting of RGS1 with miR-376b-3p was certified by luciferase reporter assay; (C) RGS1 mRNA expression in miR-376b-3p-transfected MG63 and U2OS cells; (D) levels of RGS1 in miR-376b-3p-transfected MG63 and U2OS cells. N=6, P by t-test was indicated with line segments between different groups. RGS1, regulator of G protein signaling 1.