Fig 1: PAO-Sepharose capture of protein candidates from in vivo models of oxidative Stress. Proteins were assessed for changes to their redox state in hearts from mice exposed to LPS or STZ or vehicle alone. Eluates were probed with protein-specific antibodies to APIP (a), GGCT (b), TNN13K (c), PHKG1 (d) or Nek7 (e) and quantified using GelPro Analyser 3.1. A statistically significant loss (p < 0.05) of capture in the treatment vs. vehicle group was used as an index of thiol oxidation. Data represents mean ± SEM. Statistical significance was calculated using an unpaired t-test. Uncropped Western blots are included in the Supplementary information file.