Fig 1: (A and B) Representative hematoxylin and eosin photomicrograph demonstrate tumor cells with small round nuclei, mild atypia, no mitosis. Tumor cells were arranged in a diffuse pattern of columns or nests separated by a rich vascular network and no crystals of Reinke were found. “A” and “B” represent images obtained at 20× and 40× magnification, respectively. (D–F) Immunohistochemical detection of calretinin (Abcam, ab702) (C), inhibin (Abcam, ab47720) (D), vimentin (Abcam, ab92547) (E), and epithelial membrane antigen (EMA) (Dako, Clone E29) (F) in 5 µm tissue specimen obtained from the accessory tumor. Scale bar represents 100 µm.