GFP Antibody AF4240 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGFP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF4240
ApplicationsBioassay,Purification,Immunohistochemistry,Immunoprecipitation,Western Blot,Simple Western,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
TargetGFP
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHu, Ms, Rt, Multi-species
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant GFPuv, Ser2-Lys238, Accession # P42212
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ConcentrationLYOPH

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects GFP in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this GFP antibody be used for immunofluorescence applications in mouse models?

Yes, the GFP antibody has been utilized in immunofluorescence applications, as evidenced by its use in studies involving murine models, such as the work by Almishaal et al. (2017) where it was employed to validate fluorescent labels in mouse tissues.

Is this antibody effective for detecting GFP in Western blot assays?

The GFP antibody has been shown to effectively detect GFP in Western blot assays, as indicated by its application in various studies, including those that utilized it for protein detection in cell lysates.

What species can this antibody react with in experimental setups?

The GFP antibody demonstrates broad reactivity, being effective in detecting GFP across multiple species, including human, mouse, rat, and various other mammals, as well as non-mammalian species such as zebrafish and Drosophila.

How has this antibody been utilized in research related to cellular signaling pathways?

The GFP antibody has been employed in studies examining cellular signaling pathways, such as in the research by Fan et al. (2020), where it was used to analyze the expression of GFP-tagged proteins in the context of Wnt signaling during embryonic development.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Differentiation of mesenchymal stem/stromal cells into CD45+ macrophage-like cells: expanding insights into MSC plasticityiScienceMarch 20, 2026Robert M. Rusch, Yo Mabuchi, Satoru Morikawa, Yoko Ogawa, Shigeto Shimmuraanti-mouse CD45,R&D Systems,Cat# AF114; RRID: AB_442146 Goat anti-GFPuv Antibody,R&D Systems,Cat# AF4240; RRID: AB_884445 APC anti-mouse CD68 Antibody,Biolegend,Cat# 137007; RRID: AB_10575299 APC anti-mouse

(2) Endosomal chloride/proton exchangers need inhibitory TMEM9 β-subunits for regulation and prevention of disease-causing overactivityNature communicationsApril 1, 2025Planells-Cases R, Vorobeva V, Kar S, Schmitt FW, Schulte U, Schrecker M, Hite RK, Fakler B, Jentsch TJ.(according to the manufacturer’s instructions; Invitrogen): anti-GFP (Roche #11814460001, R&D Systems #AF4240), anti-TMEM9 “T9AC2” rb 1, anti-TMEM9 “T9AC2” rb 2, anti-TMEM9 “T9AC2” gp 1, anti-TMEM9 “T9Act”-rb 2,

(3) DNA Origami‐Cyanine Nanocomplex for Precision Imaging of KRAS‐Mutant Pancreatic Cancer CellsAdvanced ScienceFebruary 14, 2025Hye‐ran Moon, Yancheng Du, Sae Rome Choi, Seongmin Seo, Cih Cheng, Bennett D. Elzey, Jong Hyun Choi, Bumsoo Hanblocked with 1% BSA and primary antibody applied as Cy5 (Sigma–Aldrich, C1117) at 1:150, GFP (Bio‐Techne, AF4240) at 1:100 and Ki67/MKI67 (Bio‐Techne, MAB7617) at 1:100 overnight at 4C. After three times wash with

(4) Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signalingCell Stem CellSeptember 5, 2024Rui Chen, Rui Fan, Fei Chen, Niraimathi Govindasamy, Heike Brinkmann, Martin Stehling, Ralf H. Adams, Hyun-Woo Jeong, Ivan Bedzhov(E8E9G),Cell signalling,"Cat# 83669, RRID: AB_2800026" Goat polyclonal anti-GFP,R&D systems,"Cat# AF4240, RRID: AB_884445" Rabbit polyclonal anti-RFP,Biomol,"Cat# 600-401-379, RRID: AB_2209751" Mouse monoclonal

(5) A cell-based assay for rapid assessment of ACE2 catalytic functionScientific reportsAugust 29, 2023Meyers WM, Hong RJ, Sin WC, Kim CS, Haas K.PA520045, ThermoFisher Scientific), ACE2 (1:35 dilution, AF933, R&D Systems), or GFP (1:10 dilution, AF4240, R&D Systems) using the default separation parameters and detected with rabbit or goat chemiluminescent

(6) Extending the dynamic range of biomarker quantification through molecular equalizationNature communicationsJuly 13, 2023Newman SS, Wilson BD, Mamerow D, Wollant BC, Nyein H, Rosenberg-Hasson Y, Maecker HT, Eisenstein M, Soh HT.

(7) Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's diseaseBrain, behavior, and immunityMarch 1, 2023Erickson MA, Logsdon AF, Rhea EM, Hansen KM, Holden SJ, Banks WA, Smith JL, German C, Farr SA, Morley JE, et al.overnight at 4 °C with the following antibodies: Green fluorescent protein (GFP, Goat, R&D Systems: AF4240, 1:100), Ionized calcium binding adaptor molecule 1 (Iba1, Rabbit, WAKO: 019–19741, 1:1,000), and Glial

(8) Proteomics uncover EPHA2 as a potential novel therapeutic target in colorectal cancer cell lines with acquired cetuximab resistanceJournal of cancer research and clinical oncologyFebruary 1, 2023Torlot L, Jarzab A, Albert J, Pók-Udvari Á, Stahler A, Holch JW, Gerlinger M, Heinemann V, Klauschen F, Kirchner T, et al.anti-EPHA2 (α-EPHA2, AF3035, R&D Systems, directed against EPHA2's extracellular domain) or anti-GFP (α-GFP AF4240, R&D Systems) antibodies at 5 µg/ml. Cells were used in assays 24 h after stimulation. Migration assay Pre-treated

(9) ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathwayLife science allianceJuly 1, 2022Wróbel M, Cendrowski J, Szymańska E, Grębowicz-Maciukiewicz M, Budick-Harmelin N, Macias M, Szybińska A, Mazur M, Kolmus K, Goryca K, et al.and -polyubiquitinylated conjugates (Cat. no. BML-PW8810) from Enzo Life Sciences; anti-GFP (Cat. no. AF4240) from R&D Systems; anti-GAPDH (Cat. no. sc-25778) and anti-lamin A/C (Cat. no. sc-7292) from Santa Cruz;

(10) MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted HeartsInternational journal of molecular sciencesJanuary 8, 2022Pittenger MF, Eghtesad S, Sanchez PG, Liu X, Wu Z, Chen L, Griffith BP.

(11) Intestinal inflammation and increased intestinal permeability in Plasmodium chabaudi AS infected miceWellcome open researchJanuary 1, 2022Mooney JP, DonVito SM, Lim R, Keith M, Pickles L, Maguire EA, Wagner-Gamble T, Oldfield T, Bermejo Pariente A, Ehimiyein AM, et al.blocking with avidin and biotin for 15min (927301, Bio Legend). Slides were incubated with goat anti-GFP (AF4240, R&D Systems) diluted 1:500 in PBS/0.1%Tween+1%FBS) in a humified chamber at 4°C overnight. After 3

(12) Neonatal diabetes mutations disrupt a chromatin pioneering function that activates the human insulin geneCell reportsApril 13, 2021Akerman I, Maestro MA, De Franco E, Grau V, Flanagan S, García-Hurtado J, Mittler G, Ravassard P, Piemonti L, Ellard S, et al.anti-Glucagon,MilliporeSigma,4031-01F rabbit anti glucagon,Dako,A0565 Somatostatin,Dako,A0566 GFP,R&D Systems,AF4240 Immunoflourescence secondary antibodies,Jackson immunoResearch,"Cy3, Cy5 and Alexa flour 488" ,, Biological

(13) Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric NeuroinflammationCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Dora D, Ferenczi S, Stavely R, Toth VE, Varga ZV, Kovacs T, Bodi I, Hotta R, Kovacs KJ, Goldstein AM, et al.Hu (Abcam, Cambridge, MA; #96474), CD45 (BioLegend, San Diego, CA; #30F11), anti-GFP (R&D Systems; #AF4240), CD11b (Bio-Rad, Hercules, CA; #M1/70.15), CSF1R (Invitrogen, Waltham, MA; #PA5-25974), F4/80(BM8)

(14) Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapauseNature communicationsOctober 30, 2020Fan R, Kim YS, Wu J, Chen R, Zeuschner D, Mildner K, Adachi K, Wu G, Galatidou S, Li J, et al.610154, 1:300), mouse anti-E-Cadherin (BD Biosciences, 610182, 1:300), goat anti-GFP (R&D systems, AF4240, 1:300), rat anti-Podocalyxin (R&D systems, MAB1556, 1:300), rabbit anti-Cleaved Caspase-3 (Cell Signalling

(15) Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cellseLifeAugust 14, 2020Cendrowski J, Kaczmarek M, Mazur M, Kuzmicz-Kowalska K, Jastrzebski K, Brewinska-Olchowik M, Kominek A, Piwocka K, Miaczynska M.anti-SQSTM1/p62 (610833), anti-μ2 (611350) and anti-SEC31A (612350) from BD Biosciences; anti-GFP (AF4240) from R&D Systems; anti-SEC16A (A300-648A) from Bethyl Laboratories Inc; anti-SEC16A (HPA005684) from

(16) Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical NeuronsFrontiers in cellular neuroscienceJanuary 1, 2020Joseph NF, Grinman E, Swarnkar S, Puthanveettil SV.Synaptic Systems), anti-PSD-95 (1:1,000, MA1-045, Thermo Fisher Scientific) and anti-GFP (1:4,000, AF4240, Novus Biologicals) in the blocking solution. After overnight incubation, cells were washed thoroughly

(17) High-resolution mapping reveals that microniches in the gastric glands control Helicobacter pylori colonization of the stomachPLoS biologyMay 1, 2019Fung C, Tan S, Nakajima M, Skoog EC, Camarillo-Guerrero LF, Klein JA, Lawley TD, Solnick JV, Fukami T, Amieva MR.rotation. Antibodies and tissue-labeling reagents Goat anti-GFP (R&D Systems, Minneapolis, MN, Catalog #AF4240; RRID: AB_884445), rabbit anti-RFP (Rockland, Limerick, PA, Catalog #600-401-379; RRID: AB_2209751),

(18) Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probeNature CommunicationsDecember 1, 2018Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon, Hadley D. SikesCatalog # AF3489) (at a dilution of 1:800) or goat primary antibody for GFP (R&D Systems, Catalog # AF4240) at 4 °C overnight and then with donkey anti-goat IR 688 (Licor) (at a dilution of 1:10,000) for 1 h

(19) Agrin has a pathological role in the progression of oral cancerBritish journal of cancerJune 1, 2018Rivera C, Zandonadi FS, Sánchez-Romero C, Soares CD, Granato DC, González-Arriagada WA, Paes Leme AF.Ct-agrin and IP-control cells. Immunoprecipitation was performed at 4 °C with 2.5 μg of GFP antibody (#af4240, R&D Systems) in the presence of 30 µL of protein G-Sepharose 4 Fast Flow (GE Healthcare) for 2 h in

(20) Natural killer cells attenuate cytomegalovirus-induced hearing loss in micePLoS pathogensAugust 1, 2017Almishaal AA, Mathur PD, Hillas E, Chen L, Zhang A, Yang J, Wang Y, Yokoyama WM, Firpo MA, Park AH.prior to application of primary antibodies. Primary antibodies to GFP (goat polyclonal anti-GFP, cat# AF4240, R&D Systems, Minneapolis, MN, USA) and tdTomato (rabbit polyclonal anti- RFP, cat#600-401-379, Rockland Antibodies,

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GFP Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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GFP Antibody from R&D Systems, a Bio-Techne Brand

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