Fig 1: In adult SMGs and PGs, FGF10pos cells are restricted to the ED, SD, and GCT ducts and express ionocyte markers(A) In adult glands, FGF10pos cells (TOM, red) express E-cadherin (ECAD, green), KRT7 (green), ASCL3 (green), FOXI1 (green), NKCC1 (green), and CFTR (green), which are expressed at the apical membrane of FGF10pos cells. Nuclei labeled with DAPI (blue).(B) In adult SMG FGF10poscells (TOM, red) were found in the GCTs (red arrows) and SDs (yellow arrows) but not in the IDs (white arrows).(C) FGF10pos cells were labeled at P29/30 (TOM, red) and stained with different antibodies. Acini were labeled with claudin (orange); MECs were labeled with aSMA (green); the basement membrane was labeled with heparan sulfate (HS) (gray); ducts were labeled with pannexin-1 (PANX1); stromal cells were labeled with VIM (green); nuclei labeled with DAPI (blue).(D and E) FGF10pos cells (TOM, red) were found within ducts of SMGs (D) and PGs (E), while not in SLGs (D). FGF10pos cells were detected only within the mesenchyme.(F) IMARIS bounding box software was used to analyze shape and size of FGF10pos cells.(G) Summary of immunostaining used to label different cell types and gland compartments. Also see Figures S4 and S5, Data S6.