Immunofluorescent analysis of RAB5 using anti-RAB5 polyclonal antibody (Product # PA3-915) shows staining in HMVEC Cells.

Western blot analysis of RAB5 was performed by loading 25 µg of MCF-7 (lane 1), Hela (lane 2) and PC12 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a RAB5 polyclonal antibody (Product # PA3-915) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~24 kDa in MCF-7 and PC-12 lysates.