Fig 1: ZC3H14 restricts PAXT-mediated degradation of the SNHG19 lncRNA. (A) Western blot analysis of ZC3H14, RBM26, and tubulin proteins using total extracts prepared from HeLa cells previously transfected with the indicated combination of siRNAs. The antibodies used for Western blot analysis are shown on the right and molecular weights markers on the left. (B) RT-qPCR analysis of SNHG19 lncRNA from total RNA harvested from HeLa cells that were previously transfected with the indicated combination of siRNAs. Data and error bars represent the means and standard deviations of four independent experiments, respectively. P-values (∗) ≤0.05; (∗∗) ≤0.01; (∗∗∗) ≤0.001; (∗∗∗∗) ≤0.0001 were determined with an unpaired Student t test. (C) Western blot analysis of ZC3H14, ZFC3H1, ZC3H3, and tubulin proteins using total extracts prepared from HeLa cells previously transfected with the indicated combination of siRNAs. The antibodies used for Western blot analysis are shown on the right and molecular weights markers on the left. The asterisk (*) indicates the position of ZC3H3. (D) RT-qPCR analysis of SNHG19 lncRNA from total RNA harvested from HeLa cells that were previously transfected with the indicated combination of siRNAs. Data and error bars represent the means and standard deviations of six independent experiments, respectively. P-values (∗) ≤0.05; (∗∗) ≤0.01; (∗∗∗∗) ≤0.0001 were determined with an unpaired Student t test.