MLC-2A Antibody 311 011 from Synaptic Systems GmbH

Product Specs
ItemMLC-2A Antibody
CompanySynaptic Systems GmbH
Price $435.00Supplier Page View Company Product Page
Catalog Number311 011
ApplicationsWB, IHC, IHC-P
Quantity100 µg
Hostmouse
TargetMLC-2A
Formatlyophilized
Reactivityhuman, rat, mouse
IsotypeIgG2b
Clonalitymonoclonal
Cell Line56F5
Clone56F5
ImmunogenRecombinant protein corresponding to AA 1 to 175 from human MLC-2A
Antibody TypePrimary antibody

Questions and Answers

What species does this antibody react with, and how has it been utilized in research?

The MLC-2A antibody reacts with mouse, human, and rat tissues. It has been shown to work in applications such as immunocytochemistry and immunohistochemistry, where it has been used to assess the presence of myosin light chain 2a in various studies, including those involving human induced pluripotent stem cell-derived cardiomyocytes and cardiac tissue analysis (, , ).

Can this antibody be used to study cardiac differentiation in stem cells?

Yes, the MLC-2A antibody has been employed in studies focusing on cardiac differentiation from human pluripotent stem cells. For instance, it has been used to characterize cardiomyocytes derived from induced pluripotent stem cells, helping to confirm the expression of cardiac-specific proteins during differentiation processes (, ).

How does the MLC-2A antibody contribute to understanding cardiac diseases?

The MLC-2A antibody is instrumental in research related to cardiac diseases, particularly in studies examining hypertrophic cardiomyopathy and cardiac aging. It has been utilized to identify and characterize cardiomyocyte maturation and function, which are critical for modeling cardiac pathologies and testing potential therapeutic interventions (, ).

What are some specific applications of the MLC-2A antibody in experimental protocols?

The MLC-2A antibody has been applied in various experimental protocols, including immunostaining for the identification of cardiac myocytes in tissue sections and cell cultures. It has been referenced in protocols for differentiating human induced pluripotent stem cells into cardiomyocytes and assessing their maturation status (, ).

Synaptic Systems GmbH

Rudolf-Wissell-Str. 28

Goettingen 37079

Germany

Phone: +49-551-50556-0
+49-551-50556-384

Company Profile

Website: http://www.sysy.com/

(1) Human iPSC cardiomyocyte patch transplantation modifies extracellular matrix and fibroblast behavior after myocardial infarctioniScienceApril 17, 2026Kosuke Torigata, Ryohei Matsuura, Fumiya Nagatomo, Moe Thiha, Takao Hikita, Hiroko Iseoka, Hiromitsu Takagi, Uichi Koshimizu, Hiroki Sakakima, Satoshi Izumi, et al.Thermo Fisher Scientific, Waltham, MA), human nuclei antigen (MAB1281, Merk Millipore, Burlington, MA), MLC2a (311 011, Synaptic Systems, Göttingen, Germany), MLC2v (310 111, Synaptic Systems, Göttingen, Germany) sarcomeric

(2) Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing ApplicationsAdvanced ScienceMarch 17, 2026Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, et al.1:500), APOL1 (Genentech A.17A5, 1:300), β‐Tubulin III (Sigma Aldrich, T2200), MLC2A (Synaptic System, 311011), CD144 (BD Biosciences 555661, 1:300), MUC1 (Abcam ab15481, 1:200), P63 (R&D BAF1916, 1:40) and NKX2.1

(3) Advanced cardiotoxicity profiling using field potential imaging with UHD-CMOS-MEA in human iPSC-derived cardiomyocytesToxicological SciencesDecember 1, 2025Naoki Matsuda, Nami Nagafuku, Kazuki Matsuda, Yuto Ishibashi, Tomohiko Taniguchi, Yusaku Matsushita, Norimasa Miyamoto, Takashi Yoshinaga, Ikuro SuzukiImmusmol). Cells were incubated overnight at 4 °C with primary antibodies against MLC-2A (Synaptic Systems, 311011) and MLC-2V (ProteinTech, 10906-1-AP), both diluted 1:200 in the appropriate buffer. The following day,

(4) Stem cell-derived heterocellular atrial engineered cardiac tissue with comparisons to native human atrial myocardiumAmerican Journal of Physiology-Heart and Circulatory PhysiologyNovember 1, 2025Daniel G. P. Turner, Willem J. De Lange, Vladislav Leonov, Roman Y. Medvedev, Jenna M. Port, Jake T. Bamlett, Di Lang, Hee Jae Jang, Jianhua Zhang, Eric G. Schmuck, et al.protein expression. Primary antibodies for GAPDH (mouse monoclonal, G8795, Millipore Sigma), MLC2A (mouse monoclonal, 311–011, Synaptic Systems), and MLC2V (rabbit polyclonal, 10906–1-AP, Proteintech) have been validated previously

(5) Serine synthesis pathway regulates cardiac differentiation from human pluripotent stem cellsiScienceJuly 18, 2025Tomohiko C. Umei, Shugo Tohyama, Yuika Morita-Umei, Manami Katoh, Seitaro Nomura, Kotaro Haga, Takako Hishiki, Tomomi Matsuura, Hidenori Tani, Yusuke Soma, et al.ab45932, 1:200, Abcam), anti-sarcomeric α-actinin (MA1-22863, 1:200, Invitrogen), anti-MLC2a (C156F5 311011, 1:200, Synaptic Systems), and anti-MLC2v (ab79935, 1:200, Abcam); and the following secondary antibodies:

(6) Differential impact of substrates on myosin heavy and light chain expression in human stem cell-derived cardiomyocytes at single-cell levelJournal of Muscle Research and Cell MotilityJune 1, 2025Felix Osten, Alea K. Bodenschatz, Karina Ivaskevica, Simon Kröhn, Birgit Piep, Tim Holler, Jana Teske, Judith Montag, Bogdan Iorga, Natalie Weber, et al.polyclonal, α-huMYH6, BioGenes), β-MyHC (mouse, monoclonal, M8421, Sigma-Aldrich), MLC2a (mouse, monoclonal, 311 011, Synaptic Systems), MLC2v (rabbit, polyclonal, 10906-1-AP, Proteintech), proBNP (mouse, monoclonal,

(7) AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturingCommun BiolMay 13, 2025Prakaimuk Saraithong, Peyton Krajcarski, Yukako Kusaka, Moe Yamada, Junichi Matsumoto, Hailey Cunningham, Sama Salih, Darby Jones, Devika Baddhan, Christian Hausner, et al.used include: Cx40 (ThermoFisher; Cat #36-4900), cTnI (Abcam; ab47003), and mlc2a (Synaptic Systems; #311011). Ponceau S total protein stain was applied to the nitrocellulose membrane prior to immunoblotting and

(8) The modulation of calcium and chloride channels induces cardiomyocytes from human pluripotent stem cellsInternational Journal of Biological SciencesJanuary 1, 2025Ya Meng, Chunhao Deng, Xia Xiao, Shengnan Wei, Chengcheng Song, Jiaxian Wang, Chon Lok Lei, Weiwei Liu, Guokai ChenThe primary antibodies used were NKX2-5 (sc-14033, Santa cruz), CT3 (TNNT2 antibody, DSHB) and MLC2A (311 011, Synaptic Systems). On the following day, the cells were washed with PBS and incubated with secondary

(9) Metabolic changes of human induced pluripotent stem cell-derived cardiomyocytes and teratomas after transplantationiScienceNovember 15, 2024Yusuke Soma, Shugo Tohyama, Akiko Kubo, Tomoteru Yamasaki, Noriko Kabasawa, Kotaro Haga, Hidenori Tani, Yuika Morita-Umei, Tomohiko C. Umei, Otoya Sekine, et al.Cambridge, MA, USA, ab137346), myosin light chain 2a (MLC2a) (Synaptic Systems, Göttingen, Germany, SYSY 311-011), myosin light chain 2v (MLC2v) (Abcam, Cambridge, USA, ab79935), anti-human nuclei (Merck Millipore,

(10) Modelling myocardial ischemia/reperfusion injury with inflammatory response in human ventricular cardiac organoidsCell ProliferationOctober 8, 2024Laihai Zhang, Yun Jiang, Wenwen Jia, Wenjun Le, Jie Liu, Peng Zhang, Huangtian Yang, Zhongmin Liu, Yang Liu(Abcam, ab24525), FAP (ABclonal, A23789), MYL2 (Synaptic System, 310,003) and MYL7 (Synaptic System, 311,011). Incubation of secondary antibodies and DAPI followed. Finally, confocal microscopy was used for taking

(11) Immune response caused by M1 macrophages elicits atrial fibrillation-like phenotypes in coculture model with isogenic hiPSC-derived cardiomyocytesStem cell research & therapySeptember 4, 2024Hutschalik T, Özgül O, Casini M, Szabó B, Peyronnet R, Bártulos Ó, Argenziano M, Schotten U, Matsa E.

(12) The Efficiency of In Vitro Differentiation of Primate iPSCs into Cardiomyocytes Depending on Their Cell Seeding Density and Cell Line SpecificityInternational journal of molecular sciencesAugust 2, 2024Tereshchenko Y, Petkov SG, Behr R.Cambridge, USA #ab11370, 1:1000), myosin light chain 2a (MLC2a, Synaptic systems, Göttingen, Germany, 311 011, 1:200), myosin light chain 2v (MLC2v, Proteintech, Rosemont, IL, USA #10906–1-AP, 1:200) and α-actinin

(13) Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaquesCell Stem CellJuly 5, 2024Yongshun Lin, Noriko Sato, So Gun Hong, Kenta Nakamura, Elisa A. Ferrante, Zu Xi Yu, Marcus Y. Chen, Daisy S. Nakamura, Xiulan Yang, Randall R. Clevenger, et al.,Santa Cruz,"Cat#sc-7844, RRID:AB_2142255" Ki67,BD Biosciences,"Cat#558615, RRID:AB_647130" MLC-2A,Synaptic System,"Cat#311011, RRID:AB_2266770" MLC-2V,ProteinTech,"Cat#10906-1-AP, RRID:AB_2147453" N-cadherin,ProteinTech,"Cat#66219-1-Ig,

(14) Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturationBiophotonics DiscovMay 1, 2024Desa DE, Amitrano MJ, Murphy WL, Skala MC.

(15) Impaired Relaxation in Induced Pluripotent Stem Cell-Derived Cardiomyocytes with Pathogenic TNNI3 Mutation of Pediatric Restrictive CardiomyopathyJournal of the American Heart AssociationMarch 19, 2024Wang R, Hasegawa M, Suginobe H, Yoshihara C, Ishii Y, Ueyama A, Ueda K, Hashimoto K, Hirose M, Ishii R, et al.ab209813; 1:300), rabbit anti‐MLC2v (Proteintech, 10 906‐1‐AP; 1:300), mouse anti‐MLC2a (Synaptic Systems, 311 011; 1:300), and mouse anti‐HCM4 (Abcam, ab302675; 1:300) as the primary antibodies. Alexa Fluor 647 donkey

(16) CD151 expression marks atrial- and ventricular- differentiation from human induced pluripotent stem cellsCommunications biologyFebruary 28, 2024Nakanishi-Koakutsu M, Miki K, Naka Y, Sasaki M, Wakimizu T, Napier SC, Okubo C, Narita M, Nishikawa M, Hata R, et al.

(17) Scalable production of homogeneous cardiac organoids derived from human pluripotent stem cellsCell Rep MethodsDecember 18, 2023Moriwaki T, Tani H, Haga K, Morita-Umei Y, Soma Y, Umei TC, Sekine O, Takatsuna K, Kishino Y, Kanazawa H, et al.T,abcam,Cat#ab45932; RRID:AB_956386 Anti-MLC2v,abcam,Cat#ab79935; RRID:AB_1952220 Anti-MLC2a,Synaptic Systems,Cat#311-011; RRID:AB_887737 Anti-Vimentin-Cy3,SIGMA,Cat#C9080-2ML; RRID:AB_259142 anti-CD31,abcam,Cat#ab28364;

(18) JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytesStem cell research & therapyDecember 13, 2023Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS.used were rabbit-anti-human MLC2V (1:400; 10,906–1-AP; Proteintech) and mouse-anti-human MLC2A (1:200; 311 011; Synaptic Systems). The secondary antibodies used were Alexa Fluor 594-conjugated donkey anti-rabbit

(19) Myosin expression and contractile function are altered by replating stem cell–derived cardiomyocytesJournal of General PhysiologyNovember 6, 2023Felix Osten, Natalie Weber, Meike Wendland, Tim Holler, Birgit Piep, Simon Kröhn, Jana Teske, Alea K. Bodenschatz, Santoshi Biswanath Devadas, Kaja S. Menge, et al.regulatory light chain (MLC2) isoforms, specific primary antibodies against MLC2a (mouse, monoclonal, 311 011; Synaptic Systems) and MLC2v (rabbit, polyclonal, 10906-1-AP; Proteintech), and secondary anti-mouse

(20) Single-Cell Analysis of Contractile Forces in iPSC-Derived Cardiomyocytes: Paving the Way for Precision Medicine in Cardiovascular DiseaseInternational Journal of Molecular SciencesAugust 30, 2023Irene C. Turnbull, Apratim Bajpai, Katherine B. Jankowski, Angelo GaitasCat#10906-1-AP, 1:300), and myosin light chain 2 (MLC-2A) mouse monoclonal antibody (Synaptic Systems Cat# 311011, 1:300). The secondary antibodies were Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,

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MLC-2A Antibody

MLC-2A Antibody

MLC-2A Antibody

MLC-2A Antibody

MLC-2A Antibody

MLC-2A Antibody from Synaptic Systems GmbH

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MLC-2A Antibody from Synaptic Systems GmbH

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