Anti-Notch1 antibody [EP1238Y] ab52627 from Abcam

Product Specs
ItemAnti-Notch1 antibody [EP1238Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab52627
Antibody TypePrimary
IsotypeIgG
CloneEP1238Y
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Quantity10 UL, 40 UL, 100 UL
ClonalityMonoclonal
Conjugate/TagUnconjugated
Gene NameNOTCH1
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetNotch1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) HIF-1α/MMP-9 Axis Is Required in the Early Phases of Skeletal Myoblast Differentiation under Normoxia Condition In VitroCellsDecember 16, 2023Flaminia Chellini, Alessia Tani, Martina Parigi, Francesco Palmieri, Rachele Garella, Sandra Zecchi-Orlandini, Roberta Squecco, Chiara SassoliName,Company,Code,Dilution anti-Ki67rabbit polyclonal,"Abcam, Cambridge, UK",ab15580,1:100 (IF) anti-Notch-1rabbit monoclonal,Abcam,ab52627,1:100 (IF)1:1000 (WB) anti-MyoD (M-318)rabbit polyclonal,Santa CruzBiotechnology,sc-760,1:50 (IF)1:500

(2) Somatic mutations reveal hyperactive Notch signaling and racial disparities in prurigo nodularismedRxivSeptember 30, 2023Ahmad Rajeh, Hannah L. Cornman, Anuj Gupta, Mindy D. Szeto, Anusha Kambala, Olusola Oladipo, Varsha Parthasarathy, Junwen Deng, Sarah Wheelan, Thomas Pritchard, et al.(X0909, DAKO, Capinteria, CA, USA). The slides were then incubated with primary antibodies for Notch1 (ab52627, Abcam, 1:150) and human NICD (AF3647, R&D Systems; dilution, 1:20) at 4°C overnight, followed by reaction

(3) Extracellular Vesicle MicroRNAs From Corneal Stromal Stem Cell Enhance Stemness of Limbal Epithelial Stem Cells by Targeting the Notch PathwayInvestigative Opthalmology & Visual ScienceSeptember 28, 2023Leying Wang, Xizhan Xu, Qiankun Chen, Yuan Wei, Zhenyu Wei, Zi-Bing Jin, Qingfeng Liangdetection of the expression of the Notch pathway, the following primary antibodies were used: Notch1 (ab52627; Abcam), NICD (D3B8; Cell Signaling), Hes1 (ab108937; Abcam), and GAPDH (1E6D9; Protein Tech). Small

(4) Salsolinol improves angiotensin II‑induced myocardial fibrosis in vitro via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathwayExperimental and Therapeutic MedicineSeptember 27, 2023Xian Zhang, Ze Shao, Yuchao Ni, Feilong Chen, Xia Yu, Jiasheng Wenno. ab68153; Abcam), anti-Jagged-1 (1:1,000; cat. no. ab109536; Abcam), anti-NICD (1:1,000; cat. no. ab52627; Abcam), anti-GAPDH (1:1,000; cat. no. orb555879; Biorbyt, Ltd.) at 4˚C overnight. The following day,

(5) Targetable NOTCH1 rearrangements in reninomaNature CommunicationsSeptember 25, 2023Taryn D. Treger, John E. G. Lawrence, Nathaniel D. Anderson, Tim H. H. Coorens, Aleksandra Letunovska, Emilie Abby, Henry Lee-Six, Thomas R. W. Oliver, Reem Al-Saadi, Kjell Tullus, et al.expression. Immunohistochemistry Renal tissue from the tumour and normal kidney for case 1 was stained with the Ab52627 Abcam antibody, including 1:150 and 1:300 concentrations (Supplementary Data 4). The tissue was fixed

(6) circAGTPBP1 promotes the progression of papillary thyroid cancer through the notch pathway via the miR-34a-5p/notch1 axisiScienceSeptember 15, 2023Dai L, Zhang W, Wang Y, Yu K, Le Q, Wu X.Proteintech Group, Inc., Wuhan, China), anti-E-cadherin (cat. #20874-1-AP, Proteintech Group), anti-NOTCH1 (cat. #ab52627, Abcam), anti-Hes1 (cat. #ab71559, Abcam), anti-Jagged1 (cat. #ab300561, Abcam), and anti-GAPDH (cat.

(7) PTEN Overexpression Alters Autophagy Levels and Slows Sodium Arsenite-Induced Hepatic Stellate Cell FibrosisToxicsJuly 3, 2023Fei Huang, Guanxin Ding, Yanjie Yuan, Lijun Zhao, Wenmeng Ding, Shunhua Wuab124964), HES1 (1:1000) (Abcam Co, UK), PTEN (1:1000) (CST, 11988S), NOTCH1 (1:500) (Abcam Co., UK, ab52627), GAPDH (1:10,000) (Abcam Co., UK), and β-actin (1:10,000) (Abcam Co., UK). The blots were rinsed three

(8) Effect of the Lipoxin Receptor Agonist BML-111 on Cigarette Smoke Extract-Induced Macrophage Polarization and Inflammation in RAW264.7 CellsInternational Journal of Chronic Obstructive Pulmonary DiseaseMay 19, 2023En Cao, Jun Xu, Yuanqi Gong, Jingjing Yuan, Anbang Chen, Jiayi Liu, Yunfei Fan, Xiangyang Fan, Xiaodong KuangSan Francisco, CA, USA), caspase-1 and cleaved caspase-1 (Ab893325, CST, Boston, MA, USA), Notch-1 (Ab52627, Abcam, San Francisco, CA, USA), activated Notch-1 (Ab52301, Abcam, San Francisco, CA, USA), and Hes-1

(9) Lactobacillus brevis alleviates the progress of hepatocellular carcinoma and type 2 diabetes in mice model via interplay of gut microflora, bile acid and NOTCH 1 signalingFrontiers in ImmunologyMay 10, 2023Shujia Chen, Ping Han, Qian Zhang, Peiyan Liu, Jie Liu, Lili Zhao, Lianyi Guo, Jia Li4°C with primary antibodies against MMP9 (1:500, CatNo: ab76003, Abcam), NOTCH 1 1 (1:1000, CatNo: ab52627, Abcam), Hes1 (1:1500, CatNo: ab108937, Abcam), Math1 (1:1000, CatNo, ab249467, Abcam), and GAPDH (1:10000,

(10) Knockdown of circ_0005615 enhances the radiosensitivity of colorectal cancer by regulating the miR-665/NOTCH1 axisOpen Med (Wars)May 9, 2023Ximin Wang, Dongxu Zheng, Changting Wang, Wanhua Chenblocking with 5% skimmed milk for 2 h, the membranes were probed with primary antibodies against NOTCH1 (ab52627, 1:1,500; Abcam, Cambridge, UK) or GAPDH (ab9485, 1:2,500; Abcam) at 4°C overnight. Afterward, the membranes

(11) LncRNA H19 mediates BMP9-induced angiogenesis in mesenchymal stem cells by promoting the p53-Notch1 angiogenic signaling axisGenes & diseasesMay 1, 2023Du C, Cheng Q, Zhao P, Wang C, Zhu Z, Wu X, Gao S, Chen B, Zou J, Huang W, et al.(#20700-1-AP, Proteintech, China), Collagen Type I (Col1a1, #14695-1-AP, Proteintech, China), Notch1 (ab52627, Abcam, USA), p53 (ab1101, Abcam, USA), p-p53 (phospho S15) (ab223868, Abcam, USA) or GAPDH (#10494-1-AP,

(12) Notch signaling is activated in knee-innervating dorsal root ganglia in experimental models of osteoarthritis joint painArthritis Research & TherapyApril 15, 2023Lai Wang, Shingo Ishihara, Jun Li, Rachel E. Miller, Anne-Marie Malfaitsections. Sections were stained overnight at 4 °C with an antibody against active Notch (NICD) (Abcam Cat# ab52627, RRID:AB_881725; 1:200), followed by an Alexa Fluor 633-conjugated secondary antibody (Invitrogen, 1:1000)

(13) The stromal-tumor amplifying STC1-Notch1 feedforward signal promotes the stemness of hepatocellular carcinomaJournal of Translational MedicineMarch 31, 2023Shuya Bai, Yuchong Zhao, Wei Chen, Wang Peng, Yun Wang, Si Xiong, Aruna, Yanling Li, Yilei Yang, Shiru Chen, et al.TJ-IRB20220562). Immunohistochemistry (IHC) IHC staining with the STC1 antibody (HPA 023918) and Notch1 (Abcam, ab52627) was performed to detect the protein expression level in HCC tissues. The IHC staining score was carried

(14) Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human SkinInternational Journal of Molecular SciencesMarch 24, 2023Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon SeoCambridge, UK" ASK 1,Rabbit,1:1000,"Abcam (ab45178), Cambridge, UK" Notch 1,Rabbit,1:1000,"Abcam (ab52627), Cambridge, UK" Caspase 14,Rabbit,1:1000,"Abcam (ab174847), Cambridge, UK" Bax,Rabbit,1:1000,"Abcam

(15) Gut microbe-derived milnacipran enhances tolerance to gut ischemia/reperfusion injuryCell Reports MedicineMarch 21, 2023Fan Deng, Jing-Juan Hu, Ze-Bin Lin, Qi-Shun Sun, Yue Min, Bing-Cheng Zhao, Zhi-Bin Huang, Wen-Juan Zhang, Wen-Kao Huang, Wei -Feng Liu, et al.anti-Cyp1a1 antibody (ab79819, Abcam), anti-beta catenin antibody (ab32572, Abcam), anti-Notch1 antibody (ab52627, Abcam), and anti-Ki67 antibody (ab16667, Abcam) were used to detect protein expression in the intestinal

(16) TGFBI remodels adipose metabolism by regulating the Notch-1 signaling pathwayExperimental & Molecular MedicineMarch 1, 2023Seul Gi Lee, Jongbeom Chae, Seon Min Woo, Seung Un Seo, Ha-Jeong Kim, Sang-Yeob Kim, David D. Schlaepfer, In-San Kim, Hee-Sae Park, Taeg Kyu Kwon, et al.anti-cleaved Notch-1 (Cat. D3B8; Cell Signaling Technology (CST), Danvers, MA, USA), anti-Notch-1 (Cat. ab52627; Abcam), anti-Hes-1 (Cat. D6P2U; CST), anti-C/EBPα (Cat. ab15048; Abcam), anti-tubulin (Cat. 2148; CST),

(17) Frizzled 7 modulates goblet and Paneth cell fate, and maintains homeostasis in mouse intestineDevelopmentFebruary 15, 2023Nai-Xin Gu, Yu-Ru Guo, Sey-En Lin, Yen-Hsin Wang, I.-Hsuan Lin, Yi-Fan Chen, Yun Yen(1:1000, Cell Signaling Technology), β-catenin (1:500, BD Biosciences, 610153), Notch1 (1:100, Abcam, ab52627) and macrophages (1:100, Abcam, ab125148) detected with Dako REAL EnVision/HRP secondary antibodies

(18) CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinomaCell death & diseaseFebruary 11, 2023Shen Y, Chen Y, Lin Y, Li Y, Liu P, Zhang B, Wang Y, Chan KC, Mak NK, Kahn M, et al.anti-ALDH1 (1:1000; Cell Signaling Technology, cat. no. 54135), anti-Notch1 (1:1000, Abcam, cat. no. ab52627), anti-EZH2 (1:1000; Cell Signaling Technology, cat. no. 5246), anti-CCND1 (1:1000, Cell Signaling Technology,

(19) Fetal pulmonary hypertension: dysregulated microRNA-34c-Notch1 axis contributes to impaired angiogenesis in an ovine modelPediatric ResearchFebruary 1, 2023Devashis Mukherjee, Ujala Rana, Alison J. Kriegel, Pengyuan Liu, Teresa Michalkiewicz, Girija Ganesh Konduriusing ImageJ and normalized to beta-actin. Primary antibodies used were rabbit Notch1 (1:1000, Abcam #ab52627), rabbit Hey1 (1:1000, Abcam catalog # 154077) and rabbit Hes1 (1:1000, Abcam catalog # ab71559). Angiogenesis assays

(20) GBP2 promotes M1 macrophage polarization by activating the notch1 signaling pathway in diabetic nephropathyFrontiers in immunologyJanuary 1, 2023Li X, Liu J, Zeng M, Yang K, Zhang S, Liu Y, Yin X, Zhao C, Wang W, Xiao L.1:1000), anti-NOS (D6B6S, Cell Signaling Technology, 1:1000), TNF-α (ab6671, Abcam, 1:1000), Notch1 (ab52627, Abcam, 1:1000), N1ICD (ab52301, Abcam, 1:1000), and GAPDH (10494-1-AP, Proteintech, 1:1000). Horseradish

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(1)

2/5/2016Excellent Anti-Notch1 Antibody from Abcam Chandan KumarMouse pups were exposed to low oxygen for 5 days, retinal extract were prepared, an equal amount of proteins were resolved in 7.5 % SDS-PAGE, and immunoblotted with anti-Notch1 antibody purchased from... Read Review
(1)

4/13/2015Good antibody to detect Notch1 Benjamin GerovacUsed this antibody to detect Notch1 protein from whole cell lysates. Antibody recognized a single band at the molecular weight shown from the company and had little background signal.

Anti-Notch1 antibody [EP1238Y] from Abcam

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Anti-Notch1 antibody [EP1238Y] from Abcam

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