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Antibodies
Antibodies
Anti-Notch1 antibody [EP1238Y]
Anti-Notch1 antibody [EP1238Y] from Abcam
(2)
Write a review
Citations:
(179)
Supplier Page
Supplier Page from
Abcam for
Anti-Notch1 antibody [EP1238Y]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Notch1 antibody [EP1238Y]
Company
Abcam
Price
Supplier Page
View Supplier Product Page
Catalog Number
ab52627
Antibody Type
Primary
Isotype
IgG
Clone
EP1238Y
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Host
Rabbit
Quantity
10 UL, 40 UL, 100 UL
Clonality
Monoclonal
Conjugate/Tag
Unconjugated
Gene Name
NOTCH1
Validation
KnockOut Validated
Reactivity
Human, Mouse
Target
Notch1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (179)
(101)
Notch1 regulates the initiation of metastasis and self-renewal of Group 3 medulloblastoma
Nature Communications
December 1, 2018
Suzana A. Kahn et al.
were processed for standard hematoxylin and eosin
staining
or processed for Notch1
staining
(
Abcam
ab52627
). Antigen retrieval was done in citrate buffer and blocking was done in 10%
horse
serum. The secondary
(102)
Notch signaling triggered via the ligand DLL4 impedes M2 macrophage differentiation and promotes their apoptosis
Cell Communication and Signaling
December 1, 2018
Sylvain Pagie, Nathalie Gérard, Béatrice Charreau
Notch1 receptor analysis,
macrophages
were immunostained using rabbit anti-Notch1 (
1:100
dilution,
ab52627
from
Abcam
, Cambridge, UK) Abs and Alexa-488 labeled anti-rabbit IgG as secondary antibodies. For apoptosis
(103)
HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
November 23, 2018
Cheng H, Zhu H, Cao M, Lu C, Bao S, Pan Y.
dry milk. Subsequently, the
membrane
was incubated with primary antibodies (HtrA:
ab38610
, Notch-1:
ab52627
, diluted
1:1000
in PBS;
Abcam
, USA) followed by the appropriate peroxidase-coupled secondary antibody
(104)
Molecular basis of Mitomycin C enhanced corneal sensory nerve repair after debridement wounding
Scientific reports
November 16, 2018
Stepp MA, Pal-Ghosh S, Tadvalkar G, Li L, Brooks SR, Morasso MI.
antibodies: βIII tubulin (
Biolegend
, #
801201
), α314, L1CAM (
Millipore
, #
MAB5272
), Notch 1 (
Abcam
, #
ab52627
),
P21
(
Santa Cruz
, #
SC6246
),
PLK1
(
Santa Cruz
, #
SC17783
), Iba1 (
Wako
, #
019-19741
),
Serpine2
(
Proteintech
,
(105)
MicroRNA‑495 targets Notch1 to prohibit cell proliferation and invasion in oral squamous cell carcinoma
Molecular Medicine Reports
November 1, 2018
Longkun Lv, Qiang Wang, Yucheng Yang, Honghai Ji
(TBST), the
membranes
were incubated overnight at 4˚C with primary antibodies against Notch1 (cat. no.
ab52627
;
1:1,000
dilution;
Abcam
, Cambridge, UK) or GAPDH (cat. no.
ab181603
;
1:1,000
dilution;
Abcam
). Following
(106)
Tsc1
Regulates the Balance Between Osteoblast and Adipocyte Differentiation Through Autophagy/Notch1/β‐Catenin Cascade
Journal of Bone and Mineral Research
November 1, 2018
Han Kyoung Choi, Hebao Yuan, Fang Fang, Xiaoxi Wei, Lu Liu, Qing Li, Jun‐Lin Guan, Fei Liu
Sigma-Aldrich
, St. Louis, MO, USA; anti-a-Tubulin: #
T6199
,
Sigma-Aldrich
, St. Louis, MO, USA; and anti-Notch1: #
ab52627
;
Abcam
, Cambridge, MA, USA. BMSC
adipocyte
differentiation BMSCs were seeded at 400,000 cells/well
(107)
MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts
Brazilian Journal of Medical and Biological Research
October 18, 2018
Xingguo Zheng, Jinhua Dai, Haijun Zhang, Zhibin Ge
(108)
Autophagy suppresses self-renewal ability and tumorigenicity of glioma-initiating cells and promotes Notch1 degradation
Cell death & disease
October 18, 2018
Tao Z, Li T, Ma H, Yang Y, Zhang C, Hai L, Liu P, Yuan F, Li J, Yi L,
et al
.
(109)
Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells
Cell Proliferation
October 1, 2018
Nanxin Liu, Mi Zhou, Qi Zhang, Li Yong, Tao Zhang, Taoran Tian, Quanquan Ma, Shiyu Lin, Bofeng Zhu, Xiaoxiao Cai
USA OPN
ab91655
Abcam
, Cambridge, MA, USA Runx-2
ab92336
Abcam
, Cambridge, MA, USA Biosystems, Shanghai, China). Notch-1
ab52627
Abcam
, Cambridge, MA, USA 2.7 |
Immunofluorescent staining
Hey-1
ab22614
Abcam
, Cambridge, MA,
(110)
Shear stress promotes differentiation of stem cells from human exfoliated deciduous teeth into endothelial cells via the downstream pathway of VEGF-Notch signaling
International journal of molecular medicine
October 1, 2018
Wang P, Zhu S, Yuan C, Wang L, Xu J, Liu Z.
containing 0.05% Tween-20 (TBS-T). It was further incubated overnight at 4˚C with antiNotch1 (
1:2,000
;
ab52627
), anti-DLL4 (
1:1,000
;
ab7280
), anti-Hey1 (2 µg/ml,
ab22614
), anti-Hey2 (2 µg/ml;
ab25404
) all from
(111)
BMP9 overexpressing adipose‑derived mesenchymal stem cells promote cartilage repair in osteoarthritis-affected knee joint via the Notch1/Jagged1 signaling pathway
Experimental and Therapeutic Medicine
September 18, 2018
Xinwei Liu, Mingchang Du, Yu Wang, Songbo Liu, Xianmin Liu
transferred to a PVDF
membrane
. After addition of type II collagen (
ab34712
), aggrecan (
ab3778
),
notch1
(
ab52627
), Jagged1 (
ab7771
), and GAPDH (
ab181602
; all
Abcam
) protein samples were incubated at 4˚C overnight
(112)
NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway
Frontiers in Molecular Neuroscience
July 18, 2018
Haiyang Xu, Yu Zhang, Ling Qi, Lijuan Ding, Hong Jiang, Hongquan Yu
western blotting detection
system (Bio-Rad) for detection. Primary antibodies used were those against NOTCH1 (#
ab52627
,
1:1000
,
Abcam
, Hong Kong, China), Jagged1 (#
ab7771
,
1:500
,
Abcam
), Hes1 (#
ab71559
,
1:500
,
Abcam
),
Hes5
(113)
HepaCAM inhibits the malignant behavior of castration-resistant prostate cancer cells by downregulating Notch signaling and PF-3084014 (a γ-secretase inhibitor) partly reverses the resistance of refractory prostate cancer to docetaxel and enzalutamide in vitro
International journal of oncology
July 1, 2018
Du Z, Li L, Sun W, Wang X, Zhang Y, Chen Z, Yuan M, Quan Z, Liu N, Hao Y,
et al
.
(anti-
HepaCAM
,
1:200
; cat. no.
18177-1-AP
;
ProteinTech
, Wuhan, China; anti-Notch1,
1:200
, cat. no.
ab52627
; anti-
Hes1
,
1:200
, cat. no.
ab108937
; both from
Abcam
, Cambridge, UK).
Staining
scoring was semi-quantitatively
(114)
Yes‑associated protein 1 promotes the metastasis of U251 glioma cells by upregulating Jagged‑1 expression and activating the Notch signal pathway
Experimental and Therapeutic Medicine
June 19, 2018
Bin Hao, Xin Chen, Yiqun Cao
antibodies were anti‑YAP1 (cat. no.
ab56701
), anti‑
JAG1
(cat. no.
ab109536
), anti‑NOTCH1 (cat. no.
ab52627
), anti‑NICD (cat. no.
ab8925
), anti‑
HES1
(cat. no.
ab108937
) and anti‑ β ‑actin (cat. no.
ab1801
) (all from
(115)
Chemotherapy Induces Breast Cancer Stemness in Association with Dysregulated Monocytosis
Clinical cancer research : an official journal of the American Association for Cancer Research
May 15, 2018
Liu L, Yang L, Yan W, Zhai J, Pizzo DP, Chu P, Chin AR, Shen M, Dong C, Ruan X,
et al
.
following antibodies and dilutions: Ki-67 (#M7240; Dako, Carpinteria, CA),
1:50
dilution;
NOTCH1
(#
ab52627
;
Abcam
),
1:35
dilution; ALDH1 (#
611194
;
BD Biosciences
),
1:100
dilution; and
CCR2
(#
ab176390
;
Abcam
),
(116)
Down-regulation of microRNA-23b aggravates LPS-induced inflammatory injury in chondrogenic ATDC5 cells by targeting PDCD4
Iranian journal of basic medical sciences
May 1, 2018
Yang Z, Tang Y, Zhao Q, Lu H, Xu G.
anti-
inhibitor
of nuclear factor κB α (IκBα,
ab32518
), anti-phosphorylated IκBα (p-IκBα,
ab133462
), anti-Notch1 (
ab52627
), anti-
Notch2
(
ab137665
), anti-Notch3 (
ab23426
), anti-GAPDH (
ab181603
) (all
Abcam
, Cambridge, UK), anti-pro
(117)
FBW7 is associated with prognosis, inhibits malignancies and enhances temozolomide sensitivity in glioblastoma cells
Cancer Science
April 6, 2018
Jing Lin, Aihui Ji, Guanzhong Qiu, Huaizhi Feng, Jian Li, Shuo Li, Yongxiang Zou, Yong Cui, Chaoli Song, Hua He, Yicheng Lu
from
Abcam
as follows:
FBW7
(
ab109617
), Ki‐67 (
ab15580
), Aurora B (
ab45145
), Mcl‐1 (
ab32087
), Notch‐1 (
ab52627
), Caspase‐3 (
ab13586
) and beta‐actin (
ab6276
). 2.7 Quantitative reverse transcription‐
polymerase chain reaction
(118)
microRNA-203 Modulates Wound Healing and Scar Formation via Suppressing Hes1 Expression in Epidermal Stem Cells
Cellular Physiology and Biochemistry
February 1, 2018
Ziheng Zhou et al.
Abcam
, Shanghai, China),
anti-Jagged1
(
1:500
,
ab7771
;
Abcam
, Shanghai, China), anti-Notch1 (
1:1
, 000,
ab52627
;
Abcam
, Shanghai, China), and anti-
Hes1
(
1:1
, 000,
ab71559
;
Abcam
, Shanghai, China). The
membranes
were
(119)
LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through Up-Regulating KLF2 Expression in ATDC5 Chondrocytes
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
January 1, 2018
Li F, Sun J, Huang S, Su G, Pi G.
(
ab194726
),
inhibitor
of nuclear factor κB α (IκBα,
ab32518
), phospho-IκBα (p-IκBα,
ab133462
), Notch1 (
ab52627
),
Notch2
(
ab137665
), Notch3 (
ab23426
), GAPDH (
ab181603
) (all from
Abcam
, Cambridge, UK), pro
caspase-9
(120)
Human induced pluripotent stem cell‑derived mesenchymal stem cells alleviate atherosclerosis by modulating inflammatory responses
Molecular medicine reports
January 1, 2018
Shi H, Liang M, Chen W, Sun X, Wang X, Li C, Yang Y, Yang Z, Zeng W.
were supplied by
Cell Signaling Technology
, Inc. (Danvers, MA, USA). Anti-Notch1 rabbit mAb (cat. no.
ab52627
) and anti-CD68 mouse mAb (cat. no.
ab955
) were purchased from
Abcam
(Cambridge, UK). Mouse IL-6 ELISA
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Reviews
Write a Review
(1)
2/5/2016
Excellent Anti-Notch1 Antibody from Abcam
Chandan Kumar
Mouse pups were exposed to low oxygen for 5 days, retinal extract were prepared, an equal amount of proteins were resolved in 7.5 % SDS-PAGE, and immunoblotted with anti-Notch1 antibody purchased from...
Read Review
(1)
4/13/2015
Good antibody to detect Notch1
Benjamin Gerovac
Used this antibody to detect Notch1 protein from whole cell lysates. Antibody recognized a single band at the molecular weight shown from the company and had little background signal.
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Anti-Notch1 antibody [EP1238Y] from Abcam
Supplier Page
View Supplier Product Page
Product Specs
Company Info
Citations
Reviews
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