Fig 2: Overexpression of RNF180 decreased the activity of glycolysis and proliferation of human H292 cells. A Functional analysis indicated that RNF180 was enriched in glycolysis metabolism in NSCLC. B Overexpression of RNF180 significantly suppressed the level of ECAR in NSCLC cells. C CCK8 assay was performed to examine the proliferation of oeNC and oeRNF180 transfecting cells respectively. D Western blot was used to examine the protein levels of C-myc, HK2, and LDHA in oeNC and oeRNF180 transfecting cells

Fig 3: RNF180 interacted with c-Myc and enhanced its ubiquitination in NSCLC cells. A Functional analysis indicated that RNF180 negatively correlated with C-myc. B qRT-PCR was used to examine the relative mRNA levels of C-myc in oeNC and oeRNF180 transfecting H358 and H1975 cells. C Western blot was used to examine the protein levels of C-myc in oeNC or oeRNF180 transfecting cells with or without the treatment of the proteasome inhibitor MG132. D CO-IP assay indicated that RNF180 interacted with C-myc. E Overexpression of RNF180 enhanced the ubiquitination of C-myc in NSCLC cells

Fig 4: RNF180 was downregulated in non-small cell lung cancer (NSCLC) and positively correlated with its overall survival rate. A RNF180 expression in patients with lung adenocarcinoma (LUAD) (n = 515) and healthy individuals (n = 59) was analyzed based on the TCGA database. *** p<0.001 vs para-cancerous. B Survival probability of patients with LUAD exhibiting high RNF180 expression (n = 126) and low-to-medium RNF180 expression (n = 376) was analyzed using the Kaplan–Meier method. C RNF180 mRNA levels in 30 pairs of tumorous and adjacent normal tissues were measured using qRT-PCR. *** p<0.001 vs para-cancerous. D Representative IHC image of RNF180 expression in tissues of healthy controls (n = 5) and patients with NSCLC (n = 93) was assessed using the IHC assay (original magnification 200×). Representative IHC image is presented. E Overall survival probability of patients with NSCLC was analyzed using the Kaplan–Meier method and compared between high RNF180-expressed (n = 53) and low RNF180-expressed (n = 40) groups