RANK/TNFRSF11A Antibody (9A725) - BSA Free NB100-56508 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemRANK/TNFRSF11A Antibody (9A725) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNB100-56508
ImmunogenA fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830)
NCBI Gene AliasesODFR, OFE, TRANCE-R, FEO, CD265, PDB2, RANK, OPTB7, TRANCER, LOH18CR1, OSTS
Concentration1.0 mg/ml
FormatPBS
ReactivityAvian,Human,Mouse,Rat
Conjugate/TagUnconjugated
Clone9A725
Quantity0.1 mg (also 0.025 mg)
HostMouse
ApplicationsFlow Cyomtetry,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Flow (Intracellular),CyTOF-ready
ClonalityMonoclonal
IsotypeIgG1 Kappa
TargetRANK/TNFRSF11A
NCBI Full Gene NameTNF receptor superfamily member 11a
PurityProtein G purified
Research AreaCell Cycle and Replication
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 mg (also 0.025 mg)

Questions and Answers

Can this antibody be used to study osteoclastogenesis in my experiments?

Yes, the RANK/TNFRSF11A Antibody has been utilized in studies related to osteoclastogenesis. For instance, it was mentioned in research where it was used to analyze the role of RANK in extracellular vesicles derived from osteoclasts, suggesting its relevance in understanding the mechanisms of osteoclast formation and regulation ().

What species can I use this antibody with in my research?

This antibody is reactive with several species, including human, mouse, chicken (bird), and rat. This broad reactivity allows for its application in various experimental models across different species.

Is there any evidence of this antibody being effective in flow cytometry applications?

Yes, the RANK/TNFRSF11A Antibody has been shown to work in flow cytometry applications, as indicated by its use in studies that involve analyzing cell populations and their differentiation states, particularly in the context of osteoclasts and their precursors.

How does this antibody perform in Western blotting?

The RANK/TNFRSF11A Antibody has been cited in studies that employed Western blotting to detect RANK protein levels, demonstrating its effectiveness in this application. For example, it was used to assess the expression of RANK in various cellular contexts, providing insights into its role in signaling pathways related to osteoclastogenesis ().

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) RANKL/PD-1 dual blockade demonstrates survival benefit for patients with advanced lung adenocarcinoma harboring KRAS mutationsCell reports. MedicineJuly 15, 2025Li HS, Liu CM, Zheng SF, Wu P, Xu HY, Hao XZ, Li JL, Xing PY, Duan JC, Wang ZJ, et al.are listed in Table S3. The primary antibodies used for Western blotting in this study include RANK (#NB100-56508) and RANKL (#NB100-56512) from Novus Biologicals (Centennial, USA); PD-L1 (#13684T), Erk1/2 (#4695),

(2) RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis PatientsBioMed research internationalJanuary 1, 0001"Nanke Y, Kobashigawa T, Yago T, Kawamoto M, Yamanaka H, Kotake S."

(3) Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanismThe Journal of biological chemistryJanuary 1, 0001"Bharti AC, Takada Y, Shishodia S, Aggarwal BB."

(4) Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quailsJournal of molecular histologyJanuary 1, 0001"Hiyama S, Yokoi M, Akagi Y, Kadoyama Y, Nakamori K, Tsuga K, Uchida T, Terayama R."

(5) Osteoblasts contribute to a protective niche that supports melanoma cell proliferation and survivalPigment cell & melanoma researchJanuary 1, 0001"Ferguson J, Wilcock DJ, McEntegart S, Badrock AP, Levesque M, Dummer R, Wellbrock C, Smith MP."

(6) c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeletonMolecular and cellular biologyJanuary 1, 0001"Izawa T, Zou W, Chappel JC, Ashley JW, Feng X, Teitelbaum SL."

(7) Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis"Journal of neural transmission (Vienna, Austria : 1996)"January 1, 0001"Kaneko YS, Ota A, Nakashima A, Nagasaki H, Kodani Y, Mori K, Nagatsu T."

(8) Signal peptide mutations in RANK prevent downstream activation of NF-κBJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchJanuary 1, 0001"Crockett JC, Mellis DJ, Shennan KI, Duthie A, Greenhorn J, Wilkinson DI, Ralston SH, Helfrich MH, Rogers MJ."

(9) Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulationJournal of periodontal researchJanuary 1, 0001"Ayon Haro ER, Ukai T, Yokoyama M, Kishimoto T, Yoshinaga Y, Hara Y."

(10) "Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL"European journal of immunologyJanuary 1, 0001"Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hänsch GM."

(11) Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient miceScientific reportsJanuary 1, 0001"Watari K, Shibata T, Nabeshima H, Shinoda A, Fukunaga Y, Kawahara A, Karasuyama K, Fukushi J, Iwamoto Y, Kuwano M, et al."

(12) RANK receptor oligomerisation in the regulation of NFκB signallingJournal of molecular endocrinologyJanuary 1, 0001"Das S, Sepahi I, Duthie A, Clark S, Crockett JC."

(13) An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examplesBiotechnology lettersJanuary 1, 0001"Huang G, Wen Q, Gao Q, Zhang F, Bai Y."

(14) Follicle-stimulating hormone promotes RANK expression on human monocytesCytokineJanuary 1, 0001"Cannon JG, Kraj B, Sloan G."

(15) Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic ratsPathogens and diseaseJanuary 1, 0001"Lafferty MK, Fantry L, Bryant J, Jones O, Hammoud D, Weitzmann MN, Lewis GK, Garzino-Demo A, Reid W."

(16) Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell linesBloodJanuary 1, 0001"Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A."

(17) Characterization of Regulatory Extracellular Vesicles from OsteoclastsJournal of dental researchJanuary 1, 0001"Huynh N, VonMoss L, Smith D, Rahman I, Felemban MF, Zuo J, Rody WJ Jr, McHugh KP, Holliday LS."were obtained from EMD Millipore (Darmstadt, Germany; 04-1507), Novus Biologicals (Littleton, CO, USA; NB100-56508), and Biorbyt (Berkeley, CA, USA; Orb6560). Anti-EpCAM (D269-3) was obtained from MBL International

(18) "The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model"BiologyJanuary 1, 0001"Dorchin-Ashkenazi H, Ginat-Koton R, Gabet Y, Klein Y, Chaushu S, Dorchin H, Brosh T, Vered M."antigen; manufacturer; concentration,"Mouse; Novus Biologicals, Centennial, CO, USA; Catalog number NB100-56508; 1:200","Rabbit; Novus Biologicals, Centennial, CO, USA; Catalog number NBP1-30042; 1:200","Rabbit;

(19) Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634Journal of orthopaedic translationJanuary 1, 0001"Ji W, Lu Y, Ma Z, Gan K, Liu Y, Cheng Y, Xu J, Liu S, Guo Y, Han S, et al."(ab19139, 1/1000; Abcam), p-IKB (ab133462, 1/1000; Abcam), IKB (ab32518, 1/1000; Abcam), TNFRSF11 (NB100-56508, 1/1000; Novus Biologicals, Littleton, CO, USA), p-IKKα (ab38515, 1/1000; Abcam), IKKα (ab32041, 1/1000;

(20) Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Lee B, Kim TH, Jun JB, Yoo DH, Woo JH, Choi SJ, Lee YH, Song GG, Sohn J, Park-Min KH, et al."

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