Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.