Fig 2: Ectopic expression of CCDC80 reducing migration, colony formation, and EMT in pancreatic cancer cells. (A) Transwell migration assay measuring migration abilities of PANC-1 and Panc02 cells expressing LPCX or CCDC80. (B) Representative images of colonies stained with methylene blue in PANC-1 and Panc02 cells stably expressing CCDC80. (C) qRT-PCR results showing down-regulation of EMT marker expression in PANC-1 and Panc02 cells by ectopic expression of CCDC80. All the data is represented as the mean of three repeated values. ***P < 0.0005, **P < 0.005, and *P < 0.05 compared to the control. (D) Western blot analysis showing reduction of EMT markers in PANC-1 and Panc02 with stably expressing CCDC80. The blots are cropped, and the original blots are presented in Supplementary Fig. 8. Note that overexpression of CCDC80 decreases EMT marker expression in pancreatic cancer cells.