Fig 1: Validation of candidate gene expression in clinical tissue samples. (A–E) Representative images of IHC staining in clinical samples. (A) ABCF3; (B) FGFR2; (C) IGSF11; (D) P2RY6; (E) SOX2. The upper row of each figure represents tumor tissues and the lower row represents normal tissues. Scale bar = 20 µm: magnification = ×10; Scale bar = 100 µm: magnification = ×40. (F–I) The expression levels of ACSL5 (F), FGFR2 (G), LAMC2 (H) and P2RY6 (I) in normal and LUSC tissues in our own RNA-seq database. **P < .01, ***P < .001. IHC = immunohistochemistry, LUSC = lung squamous cell carcinoma, ns = not significant.