U2AF65 (MC3) Antibody sc-53942 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemU2AF65 (MC3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-53942
Antibody TypePrimary Antibodies
NCBI Gene AliasesU2AF65, 65kDa
Quantity200 µg/ml
CloneMC3
Reactivitymouse, rat, human
ApplicationsWB, IP, IF, IHC(P)
ClonalityMonoclonal
Conjugate/TagUnconjugated
FormatPurified
Hostmouse
NCBI Full Gene NameU2 small nuclear RNA auxiliary factor 2, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
TargetU2AF65
IsotypeIgG2b

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.rocking, using the following dilutions: FLAG at 1:1000 (Sigma, F3165), U2AF2 at 1:1000 (Santa Cruz, sc-53942), EIF3J at 1:500 (Bethyl, A301–746A-M), GIGYF2 at 1:500 (Bethyl, A303–732A), and G3BP1 at 1:2000 (Invitrogen,

(2) TDG orchestrates ATF4-dependent gene transcription during retinoic acid-induced cell fate acquisitionNucleic Acids ResMarch 3, 2026Marion Turpin, Thierry Madigou, Maud Bizot, Rachael Acker, Stephane Avner, Gérard Benoît, Martin Braud, Cynthia Fourgeux, Gaëlle Palierne, Jeremie Poschmann, et al.and immunoblotting experiments performed as described previously [52]. Rabbit antibodies against U2AF65 (sc-53942) were purchased from Santa-Cruz Biotechnologies, p70S6K (#9202) and T389p70S6K-Ph (#9234) from Cell Signalling

(3) Protocol for visualization of ultrafine nuclear structures in cultured cells using protein retention expansion microscopySTAR ProtocolsSeptember 19, 2025Takaaki Okamoto, Masahide Fukada, Akio Masudaanti-PTBP1∗,Proteintech,"Cat#12582-1-AP, RRID:AB_2176402" Mouse monoclonal anti-U2AF2,Santa Cruz,"Cat#sc-53942, RRID:AB_831787" Normal mouse IgG,Santa Cruz,"Cat#sc-2025, RRID:AB_737182" Normal rabbit IgG,Santa Cruz

(4) AEP-cleaved DDX3X induces alternative RNA splicing events to mediate cancer cell adaptation in harsh microenvironmentsJournal of Clinical InvestigationFebruary 1, 2024Wenrui Zhang, Lu Cao, Jian Yang, Shuai Zhang, Jianyi Zhao, Zhonggang Shi, Keman Liao, Haiwei Wang, Binghong Chen, Zhongrun Qian, et al.(Proteintech, Cat 12987-1-AP, RRID: AB_2136290), anti-U2AF2 antibody (Santa Cruz Biotechnology, Cat sc-53942, RRID: AB_831787), anti-SRSF1 antibody (Santa Cruz Biotechnology, Cat sc-33652, RRID: AB_628248), anti-hnRNPA1

(5) MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongationNucleic acids researchAugust 25, 2023Jin B, Zhu J, Pan T, Yang Y, Liang L, Zhou Y, Zhang T, Teng Y, Wang Z, Wang X, et al.Abcam, 8 μg/IP), p-Pol II (Ser2) (ab5095, Abcam, 5 μg/IP), p-Pol II (Ser5) (2629, CST, 5 μg/IP), U2AF65 (sc-53942, Santa Cruz, 5 μg/IP) and SNRPA (10212–1-AP, Proteintech, 5 μg/IP). DNA–RNA immunoprecipitation (DRIP)

(6) Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear exportCell reportsJuly 19, 2022Duan L, Zaepfel BL, Aksenova V, Dasso M, Rothstein JD, Kalab P, Hayes LR.1:500),Millipore,Cat# 05-1588; RRID:AB_10805959 Mouse anti-U2AF65 (IF 1:100),Santa Cruz Biotechnology,Cat# sc-53942; RRID:AB_831787 Mouse anti-UAP56 (BAT1) monoclonal (IF 1:50),Santa Cruz Biotechnology,Cat# sc-271395;

(7) hnRNPK-derived cell-penetratingpeptide inhibits cancer cell survivalMolecular therapy oncolyticsDecember 17, 2021Puvvula PK, Buczkowski S, Moon AM.

(8) Towards Splicing Therapy for Lysosomal Storage Disorders: Methylxanthines and Luteolin Ameliorate Splicing Defects in Aspartylglucosaminuria and Classic Late Infantile Neuronal Ceroid LipofuscinosisCellsOctober 20, 2021Banning A, Tikkanen R.method. 2.4. Antibodies Mouse monoclonal antibodies against firefly luciferase (sc-74548) and U2AF65 (sc-53942) were from Santa Cruz Biotechnology (Heidelberg, Germany). A mouse monoclonal antibody against GAPDH

(9) Interleukin enhancer-binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanomaClinical and translational medicineOctober 1, 2021Zhang X, Bustos MA, Gross R, Ramos RI, Takeshima TL, Mills GB, Yu Q, Hoon DSB.ILF2 Ab (1:250 dilution, Santa Cruz, Cat# sc‐365068) and U2AF2 Ab (1:200 dilution, Santa Cruz, Cat# sc‐53942). Images were taken by the BX43 upright microscope (Olympus, Tokyo) at 20× magnification and with the

(10) Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicingMolecular neurodegenerationSeptember 22, 2021Zhang Q, Zhang J, Ye J, Li X, Liu H, Ma X, Wang C, He K, Zhang W, Yuan J, et al.(Santa Cruz Biotechnology, #sc-514655) at dilution of 1:1,000 dilution, mouse anti-U2AF65 (Santa Cruz Biotechnology, #sc-53942) at dilution of 1:1,000, mouse anti-U2AF35 (Proteintech, #60289-1-Ig) at dilution of 1:2,500, rabbit

(11) Huntington's disease-specific mis-splicing unveils key effector genes and altered splicing factorsBrain : a journal of neurologyAugust 17, 2021Elorza A, Márquez Y, Cabrera JR, Sánchez-Trincado JL, Santos-Galindo M, Hernández IH, Picó S, Díaz-Hernández JI, García-Escudero R, Irimia M, et al.Abcam, ab191528), rabbit anti-TIA1 (1:1000, Abcam, ab40693), rabbit anti-U2AF2 (1:500, Santa Cruz, sc-53942), mouse anti-RBFOX1 (1:2000, Merck Millipore, MABE985) for mouse samples, mouse anti-RBFOX1 (1:1000,

(12) Human prefoldin modulates co-transcriptional pre-mRNA splicingNucleic acids researchJune 21, 2021Payán-Bravo L, Fontalva S, Peñate X, Cases I, Guerrero-Martínez JA, Pareja-Sánchez Y, Odriozola-Gil Y, Lara E, Jimeno-González S, Suñé C, et al.anti-Rpb1 8WG16 (Thermo Fisher MA1-10882, ChIP); anti-FLAG (Sigma, F3165, ChIP); anti-U2AF65 (Santa Cruz, sc-53942, ChIP and WB); anti-PRPF19 (Lührmann Laboratory, ChIP and WB); anti-PRGL1 (Lührmann Laboratory, WB);

(13) CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanismsNucleic acids researchJune 21, 2021Batsché E, Yi J, Mauger O, Kornobis E, Hopkins B, Hanmer-Lloyd C, Muchardt C.05-690), H3K9me2 (07–441); from Santa-Cruz for Sam68 (7-1, sc-1238), ASF/SF2 (sc-33652), U2AF65 (MC3, sc-53942), RNAPII (N20, sc-899), N-cadherin (CDH2, sc-7939); from Cell Signaling for SUV39H1 (D11B6, #8729);

(14) Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferationCell reportsJune 1, 2021Puvvula PK, Yu Y, Sullivan KR, Eyob H, Rosenberg J, Welm A, Huff C, Moon AM.

(15) Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and SurvivalFrontiers in oncologyJanuary 1, 2021Puvvula PK, Moon AM.Anti-cancer Peptides hnRNPC1/C2 (Santa Cruz, SC-32308), SAFA (Santa Cruz, SC-32315), U2AF65 (Santa Cruz, SC-53942), DDX3 (Santa Cruz, SC-365768), hnRNPA1 (Santa Cruz, SC-32301), hnRNPD (abcam, ab61193), DDX21 (Santa Cruz

(16) SON and SRRM2 are essential for nuclear speckle formationeLifeOctober 23, 2020Ilik İA, Malszycki M, Lübke AK, Schade C, Meierhofer D, Aktaş T.SRSF7 (MBL, RN079PW), U1-70K (Santa Cruz Biotechnology, sc-390899), U2AF65 (Santa Cruz Biotechnology, sc-53942). Note on SC35/SRSF2 antibodies There are many commercially available antibodies that are labeled

(17) Identification of long non-coding RNAs in advanced prostate cancer associated with androgen receptor splicing factorsCommunications biologyJuly 23, 2020Takayama KI, Fujimura T, Suzuki Y, Inoue S.

(18) Cis- and trans-regulations of pre-mRNA splicing by RNA editing enzymes influence cancer developmentNature communicationsFebruary 7, 2020Tang SJ, Shen H, An O, Hong H, Li J, Song Y, Han J, Tay DJT, Ng VHE, Bellido Molias F, et al.(Santa Cruz Biotechnology, sc-28380), anti-SRSF7 (Abcam, ab138022), anti-U2AF65 (Santa Cruz Biotechnology, sc-53942), and anti-CCDC15 (Abcam, ab170396). Foci formation assay Cells were seeded to six-well plate at a

(19) The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulationGenes & developmentMay 1, 2019Palangat M, Anastasakis DG, Fei DL, Lindblad KE, Bradley R, Hourigan CS, Hafner M, Larson DR.(1:1000; Invitrogen, PAS21764), U2AF1 (1:1500; Abcam, AB172614), U2AF2 (1:1000; Santa Cruz Biotechnology, SC53942), TSC2 (1:1000; ThermoFisher, 37-0500), RUNX1 (1:1000; ThermoFisher, MA5-15814), TRIM28 (1:1000; ThermoFisher,

(20) Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5' splice-site sequences due to weak interactions with SR proteinsNucleic acids researchJanuary 25, 2019Krchnáková Z, Thakur PK, Krausová M, Bieberstein N, Haberman N, Müller-McNicoll M, Stanek D.Sciences, Prague, Czech Republic), mouse hnRNP F/H (Santa Cruz sc-32310) and mouse U2AF2 (Santa Cruz sc-53942). Cellular fractionation Cellular fractionation assays were performed as previously described (32).

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U2AF65 (MC3) Antibody

U2AF65 (MC3) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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U2AF65 (MC3) Antibody from Santa Cruz Biotechnology, Inc.

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