Fig 1: The cellular distribution and relative level of the G9A protein in the postnatal mouse testes. a Representative micrographs of G9A immunostaining in the young (n = 10), prepubertal (n = 8), pubertal (n = 8), postpubertal (n = 12), and aged (n = 17) groups. The micrographs were captured at 400 × original magnification. Scale bars are equal to 50 µm. b Relative G9A levels in the testes from 1- to 52-week-old and c postnatal testes from the young to the aged groups. d Relative G9A levels per seminiferous tubule in each age and e each group. f G9A levels in germinal epithelial cells of the postnatal testis groups. The data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. P < 0.05 was considered statistically significant. We present values as mean ± standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Y young, PreP prepubertal, P pubertal, PostP postpubertal, A aged, SG spermatogonium, EP early prophase spermatocyte, P pachytene spermatocyte, RS round spermatid, ES elongating spermatid, EdS elongated spermatid, S Sertoli cell, L lumen, IT intertubular area