BiP (C50B12) Rabbit mAb 3177S from Cell Signaling Technology

Product Specs
ItemBiP (C50B12) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3177S
Quantity100 µl
ApplicationsW, IHC-P, FC-FP
ReactivityH, M
TargetBiP
HostRabbit
NCBI Gene AliasesBIP, GRP78, HEL-S-89n
ClonalityMonoclonal
NCBI Full Gene Nameheat shock protein family A (Hsp70) member 5
Research AreaProtein Folding and Trafficking
IsotypeIgG
CloneC50B12
Molecular Weight78

Questions and Answers

Can this antibody be used to study the unfolded protein response in different cell types?

Yes, the BiP (C50B12) Rabbit mAb has been utilized in studies investigating the unfolded protein response, particularly in the context of viral infections. For instance, it was used to analyze the role of BiP in the IRE1-mediated unfolded protein response during tick-borne flavivirus infections in various human cell lines, highlighting its relevance in understanding cellular stress responses ().

Is this antibody effective for immunoblotting applications?

The BiP (C50B12) Rabbit mAb has been shown to work effectively in immunoblotting applications, as evidenced by its use in multiple studies to detect BiP protein levels in various experimental setups, including those involving endoplasmic reticulum stress and viral infections (, ).

What species can this antibody detect, and is it suitable for human studies?

This antibody is reactive with both human (Homo sapiens) and mouse (Mus musculus) BiP proteins, making it suitable for studies involving these species. Its application in human cell lines has been documented in research focusing on the unfolded protein response and its implications in diseases such as dengue and neuroblastoma (, ).

Are there any specific experimental conditions or treatments where this antibody has been particularly useful?

The BiP (C50B12) Rabbit mAb has been particularly useful in studies involving endoplasmic reticulum stress and the unfolded protein response, such as in the context of glioma cell growth inhibition and the effects of various pharmacological agents that induce ER stress (, ).

Cell Signaling Technology

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Website: Technical Support

(1) PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growthTumour Virus ResearchJune 1, 2026Shuxiang Wu, Xiaozhen Chen, Yilin Huang, Fangrong Zhang, Xinjian Lin, Xu Lin(Creative Diagnostics, #DMABT–51328MH), Flag (Cell Signaling Technology, #14793S), BIP (Cell Signaling Technology, #3177), β–actin (Cell Signaling Technology, #4970S), PRMT5 (Cell Signaling Technology, #2252S), monomethylarginine

(2) Fucoxanthin Ameliorates MASLD by Directly Targeting GRP78 to Restore ER Homeostasis and Activate AMPK SignalingFood Sci NutrApril 30, 2026Lin Zhang, Ruifen Zhang, Yu Wu, Binbin Chen, Chao Wang, Cheng Wang, Zhengshun Wen, Jingui Zhang, Haibin Tong, Liang Hongwas purchased from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies against GRP78 (#3177), eIF2α (#9722), and phospho‐eIF2α (Ser51, #3398) were purchased from Cell Signaling Technology (Beverly,

(3) Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistanceCell Oncol (Dordr)April 20, 2026Hong Jiang, Kan Li, Yahui Wang, Qiuhan Zheng, Huanzi Lu, Dikan Wang, Jianghai Chen, Guiqing Liao, Yujie LiangAnti-TIA1 (Cat No. 12133-2-AP) was purchased from Proteintech (China). Primary antibodies against BIP (#3177), PERK (#5683), IRE1α (#3294) were obtained from Cell Signaling Technology (USA). Primary antibodies

(4) RNA-binding protein ELAVL1 modulates WDR36 to inhibit p53 pathway and reduce calcium overload in retinal cells under acute pressure elevationOpen Life SciApril 20, 2026QingFeng Meng, BaoLi Ning, WenXiong Chen, JingJing Zhang, Jun FuCaspase-3 (#9661, Cell Signaling Technology), CHOP (#2895, Cell Signaling Technology), GRP78/BiP (#3177, Cell Signaling Technology), ATF4 (#11815, Cell Signaling Technology), p-PERK (Thr980) (#3179, Cell Signaling Technology

(5) Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein responseBioact MaterApril 9, 2026Yu Sun, Qifeng Chen, Yan Zhao, Yuanyuan Yin, Qiao Chen, Lixin Yang, Zixuan Liu, Rui Chen, Xiaohui Xu, Jinlin SongBeyotime) and incubated with primary antibodies against NRF2 (1:1000, ab62352, Abcam), GRP78 (1:1000, 3177T, CST), p-IRE1α (1:1000, AP1442, Abclonal), IRE1α (1:1000, 3294T, CST), XBP-1 (1:1000, WL00708, Wanleibio),

(6) Toxoplasma gondii effector GRA35 mediates neuronal damage via ER stress and mitochondria-associated apoptosisVirulenceApril 6, 2026Jie Wang, Ying Chen, Nan Zhou, Fangmin Li, Niuniu Dai, Zhiang Chen, Shutong Liu, Ran An, Lijian Chen, Jian Du9721S), ATF4 rabbit polyclonal antibody(Catalog #: 11815S), GRP78 rabbit polyclonal antibody(Catalog #: 3177S), CHOP rabbit polyclonal antibody (Catalog #: 5554S) all purchased from Cell Signaling Technology. Multicolor

(7) Tri-Ortho-Cresyl Phosphate Inhibits Proliferation of Mouse Germ Cells by Activating Endoplasmic Reticulum Stress and Suppressing NTE ActivityToxicsMarch 25, 2026Dan Yang, Di Zhang, Xiao-Hua Song, Xiang-Dong Li(Shanghai, China). The other chemicals used are detailed below. 2.2. Antibodies Antibodies against GRP78 (#3177), CHOP (#2895), BrdU (#5292), Phospho-H3 (Ser10) (#53348), and PCNA (D3H8P) were purchased from Cell Signaling Technology

(8) Targeting LIPA with ERX-41 Induces ER Stress and Inhibits Tumor Progression in Inflammatory Breast CancerBiomoleculesMarch 23, 2026Zenaida Fuentes, Gaurav Sharma, Bianca A. Romo, Rahul Gopalam, Khaled Mohamed Nassar, Paulina Ramirez, Nicole Mejia, Chia-Yuan Chen, Scott Elmore, Henry Neal, et al.Immunohistochemistry Sections were blocked, incubated overnight with Ki-67 (Abcam 16667; 1:50) and GRP78 (#3177; 1:50, Cell Signaling Technology, Inc. Danvers, MA, USA) antibodies, then treated with the ImmPRESS®

(9) DZ-1-artesunate conjugate induces mitochondria-mediated, reactive oxygen species-dependent apoptosis in colorectal cancer tumoroidsFrontiers in PharmacologyMarch 20, 2026Badrinath Narayanasamy, Yi Zhang, Alexandra Gangi, Robert Figlin, Karine Sargsyan, Heuiran Lee, Cheryn Song, Yong J. Leeanti-caspase-8 (Cat #9746), anti-cleaved caspase-9 (Cat#7237), anti-ATF-4 (Cat#11815S), anti-BiP (Cat#3177S) and anti-HO-1 (Cat#5061S) were purchased from Cell Signaling Technology. Anti-β-actin (Cat #A1978)

(10) MSMO1 promotes chemotherapy resistance through modulation of T-MAS metabolism via PERK/elF2α/ATF4/CHOP pathwayiScienceMarch 20, 2026Hengyu Ren, Xuliren Wang, Zhibo Shao, Min Xiong, Weiru Chi, Yuting Sang, Qi Zhang, Zehao Wang, Douwaner Liu, Liyi Zhang, et al.,Cell Signaling Technology,Cat# 2118; RRID:AB_561053 Rabbit monoclonal BiP antibody,Cell Signaling Technology,Cat# 3177; RRID:AB_2119845 Rabbit monoclonal ATF4 antibody,Cell Signaling Technology,Cat# 11815; RRID:AB_2616025 Rabbit

(11) Therapeutic optimization of LIPA targeting to induce endoplasmic reticulum stress and cell death in ovarian cancerOncogeneMarch 10, 2026Suryavathi Viswanadhapalli, Tae-Kyung Lee, Scott Elmore, Gaurav Sharma, Rahul Gopalam, Durga Meenakshi Panneerdoss, Xihui Liu, Karla Parra, Tanner Reese, Michael Hsieh, et al.antibodies for p-eIF2α (#3398), eIF2α (#5324), p-PERK (#3179), PERK (#5683), CHOP (#2895), GRP78 (#3177), ATF4 (#11815), and ATF6 (#65880) were acquired from Cell Signaling Technology (Danvers, MA, USA).

(12) S-nitrosylation contributes to ER stress and aggresome formation in Secosterol-B-mediated endothelial dysfunctionJournal of Lipid ResearchMarch 10, 2026Maria Gemma Nasoni, Erik Bargagni, Francesca Monittola, Pasquale Creanza, Elisa Maricchiolo, Sofia Masini, Anastasia Ricci, Serena Benedetti, Silvia Carloni, Sabrina Burattini, et al.anti-spliced X-box binding protein 1 (XBP-1s) (E9V3E, #40435), ATF6 (D4Z8V, #65880), BiP/GRP78 (C50B12, #3177), and protein disulfide isomerase (PDI) (#2446) from Cell Signaling Technology; anti-endothelial NOS

(13) Alkyltriphenylphosphonium Binding to Cardiolipin Triggers Oncosis in Cancer CellsAdvanced ScienceMarch 1, 2026Jin Li, Hang Zheng, Yuxing Lin, Ziwei Zhao, Yijia Mai, Zhangrong Lou, Qiang Liu, Zhao Ma, Chengjun Wuincluding CHOP (#2895T,), ATF‐4 (#11815S), ATF‐3 (#18665S), XBP‐1s (#12782S), IRE1α (#3294T), BiP (#3177T), PERK (#5683T), eIF2α (#5324T), p‐eIF2α (#3597S), p62 (#5114), LC3B (#2775S), and β‐actin (#3700S),

(14) Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomersNeurobiology of DiseaseMarch 1, 2026Brianna Hnath, Srinivasan Ekambaram, Nikolay V. DokholyanAntibody,Company,Catalog Number ALIX,Cell Signaling,2171 ALS2,Proteintech,"13,998–1-AP" BiP,Cell Signaling,3177 C4F6,Medimabs,MM-0070-2-P C9orf72,Proteintech,"22,637–1-AP" Capping protein (Rb anti CARMIL2),My

(15) Pharmacological Inhibition of SLC33A1 Promotes Endoplasmic Reticulum Hyperoxidation and Induces Adaptive IRE1/XBP1s SignalingbioRxivFebruary 21, 2026Sergei Kutseikin, Maria Rafiq, Prerona Bora, Shanshan Liu, Rick A. Homan, Jeffrey T. Mindrebo, Matthew Holcomb, H. Michael Petrassi, Huang Qiu, Anastasiya Redkina, et al.IRE1alpha (Cell Signaling, Cat # 3294), XBP1s (Cell Signaling, Cat # 40435), BiP (Cell Signaling, Cat # 3177), CHOP (Cell Signaling, Cat # 2895), and Tubulin (Cell Signaling, Cat # 2144). DNA constructs Sequences

(16) D-allose suppresses colitis associated carcinogenesis by reversing ER stress in macrophages and inhibiting cancer cell proliferationFrontiers in ImmunologyFebruary 20, 2026Xiaodong Li, Keizo Hiraishi, Kensuke Kumamoto, Shin-ichi Nakakita, Tetsuo Yamashita, Kazuyo Kamitori, Kiyomi Ohmichi, Ryou Ishikawa, Lin Hai KuraharaAntibody,Dilution,Manufacture,Catalog number,Purpose β-Actin,1:3000,FUJIFILM,010-27841,WB Bip,1:1000,Cell signaling,#3177,"IH, WB" CD68,1:1000,Cell signaling,#76437,IH CD206,1:1000,Abcam,Ab64693,IH CHOP,1:1000,Cell signaling,#2895,"IH,

(17) Alcohol Consumption During Muscle Disuse Causes Differential Signaling Responses in a Muscle-Specific Manner in MiceInternational Journal of Molecular SciencesFebruary 15, 2026Jinseok Lee, Deokhwa Jeong, Rudy J. Valentine(#8395, 1:1000), p-PERK (Thr980, #3179, 1:1000), PERK (#5683, 1:1000), ATF6 (#65880S, 1:1000), BIP (#3177, 1:1000), ATF4 (#11815, 1:1000), XBP1s (#40435, 1:1000), p-eIF2α (#3398, 1:1000), eIF2α (#9722, 1:1000),

(18) Epigenetic Buffering of Proteotoxic Stress by EHMT2 Enables Meningioma GrowthNeuro-Oncology AdvancesFebruary 13, 2026Bethany C Berry, Tomas Rodriguez, Connie C Hou, Madison R B Marasco, Brittany Owusu-Adjei, Gabriella M Kastrunes, Rona S Carroll, Hong Wei Yang, Mark D Johnsonfollowing antibodies were used: anti-ATF-4 (11815, 1:1000, Cell Signaling Technology), anti-HSPA5/Bip (3177, 1:1000, Cell Signaling Technology), anti-DDIT3/CHOP (2895, 1:1000, Cell Signaling Technology), anti-GADD34

(19) SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasisbioRxivFebruary 3, 2026Shanshan Liu, Mark Gad, Caifan Li, Kevin Cho, Yuyang Liu, Khando Wangdu, Viktor Belay, Alon Millet, Hiroyuki Kojima, Henry Sanford, et al.CALR (12238P), citrate Synthase (14309S), PDI (3501P), IRE1 (3294), PDIA3 (2881T), PERK (5683), BiP (3177), ATF6 (65880S), CHOP (2895), ATF4(11815S), XBP-1s (40435S), SYVN1 (14773S), HA (2367S), HA (3724S),

(20) Chlamydial membrane vesicles deliver the beta barrel outer membrane protein OmpA to mitochondria to inhibit apoptosisPLoS PathogFebruary 2, 2026Andreea Mesesan, Henry Oehler, Collins Waguia Kontchou, Aladin Haimovici, Martin Helmstädter, Oliver Kretz, Oliver Schilling, Stefan Tholen, John Atanga, Irina Nazarenko, et al.(Biozol Diagnostica, #LS-C79219), FLAG (Sigma, #F1804), GFP (Roche, #11814460001), BiP (Cell signalling, #3177), golgin-84 (SantaCruz, #sc-365337), lamin B (Abcam, #ab45848), RAB7 (Abcam, #ab50533), α-tubulin (Sigma,

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3/8/2017BiP Antibody Liyong Z.To test the stability of protein targets implicated in serum starvation, Hep G2 cells were used to perform the pulse-chase experiments by Western blotting.

BiP (C50B12) Rabbit mAb

BiP (C50B12) Rabbit mAb from Cell Signaling Technology

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BiP (C50B12) Rabbit mAb from Cell Signaling Technology

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