Goat Anti-Rabbit Ig, Human ads-HRP 4010-05 from SouthernBiotech

Product Specs
ItemGoat Anti-Rabbit Ig, Human ads-HRP
CompanySouthernBiotech
Price $104.00Supplier Page View Company Product Page
Catalog Number4010-05
Quantity1.0 mL
ApplicationsELISA, IHC-FS, IHC-PS, ICC, WB
Conjugate/TagHRP (Horseradish Peroxidase)
ReactivityRabbit
TargetIg
IsotypeIgG
HostGoat
FormatLiquid - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
ClonalityPolyclonal
ConcentrationLot specific
PurityPooled antisera from goats hyperimmunized with rabbit IgG; purified by affinity chromatography; reacts with the heavy and light chains of rabbit IgM and IgG; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity
ImmunogenRabbit IgG
Research AreaImmunology, Secondary Antibodies
Antibody TypeSecondary Antibody

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) Inhibition of the NLRP3 Inflammasome With MCC950 Improves Gut Health in Huntington's Disease MiceJ NeurochemMarch 30, 2026Sujan Kumar Sarkar, Millicent N. Ekwudo, Da Lu, Bethany Masson, Pamudika Kiridena, Nicholas van de Garde, Thibault Renoir, James E. Vince, Veerasikku Gopal Deepagan, Anthony J. Hannan, et al.1010‐05; anti‐goat secondary, Invitrogen, Cat# A16005; anti‐rabbit secondary, Southern Biotech, Cat# 4010‐05). Membranes were washed in TBS‐T 3 × 5 min and then images were captured in Chemidoc Touch Imaging System

(2) Protocol for purifying recombinant HBV capsids from HepG2 cells and comparative phosphorylation analysis by phosphate-affinity SDS-PAGESTAR ProtocolsMarch 20, 2026Katie Culhane, Shiv Kumar, Jianming Hu, Joseph Che-Yen WangCorporation,Cat. #Z2085RL-A Goat anti-rabbit HRP-conjugated antibody (1:3000),Southern Biotech,Cat. #4010-05 Mouse monoclonal anti-HBcAg antibody (1:3000),Tokyo Future Style,Clone: Hyb-T2221 Goat anti-mouse

(3) Genome-wide CRISPR Screening Reveals a PKA-Driven Resistance Mechanism to Metformin for Oral Cancer Prevention That Can Be Exploited by Combination with NSAIDsCancer Prevention ResearchFebruary 6, 2026Thomas S. Hoang, Farhoud Faraji, Amaya N. Mendez-Molina, Sendi Rafael Adame-Garcia, Kuniaki Sato, Tomohiko Ishikawa, Pham Thuy Tien Vo, Sydney I. Ramirez, Paola Y. Anguiano Anguiano Quiroz, Tracy Guo, et al.horseradish peroxidase–conjugated goat anti-rabbit IgG secondary antibody (Southern Biotech, cat. #4010-05) at a dilution of 1:20,000 in 5% milk-TBST buffer. Immunoreactive bands were visualized using the Immobilon Western Chemiluminescent HRP substrate

(4) Cryo-EM structures of HBV capsids from human cells at near-atomic resolutionStructureFebruary 5, 2026Emily N. Bianchini, Carolina Pérez-Segura, Haitao Liu, Laura Luckenbaugh, John Flanagan, Yuanheng Cai, John Shanklin, Adam Zlotnick, Jodi A. Hadden-Perilla, Jianming Hu, et al.antigen),Zeta Corporation,Cat #Z2085RL-R "Goat anti-Rabbit Ig, human ads-HRP",Southern Biotech,Cat #4010-05 ,, ,, ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains Stellar™

(5) Characterisation of Bespoke Patient-Derived In Vitro Models of Ewing SarcomaCancers (Basel)February 4, 2026Elizabeth A. Roundhill, Elton J. R. Vasconcelos, John Davies, Susan A. Burchilldetected using goat anti-mouse (1:5000, 170-6516, Bio-Rad, Watford, UK) and goat anti-rabbit (1:5000, 4010-05, Southern Biotech, Homewood, AL, USA) secondary antibodies for 1 h at room temperature. Protein bands

(6) A split biotin ligase approach reveals proteins associated with oligomeric alpha-synuclein during aggregationScientific ReportsFebruary 4, 2026Analiese R. Fernandes, Abigail P. Owen, Ayman H. Faroqi, Jannifer Lee, Gunveen S. Sachdeva, Dmytro Morderer, Cody Hoffmann, Benjamin Madden, Shuwen Zhang, Yingxue Ren, et al.being washed (3 × 5 min in TBS-T). Membranes were incubated with anti-rabbit-HRP (Southern Biotech, 4010-05) for anti-aSYN blots or anti-streptavidin-HRP (Southern Biotech, 7100-05) for anti-biotin blots for

(7) Mbd4 and MutSα protect cells from spontaneous deamination of 5-methylcytosineNucleic Acids ResearchJanuary 20, 2026Rebecca A Bilardi, Christoffer Flensburg, Zhen Xu, Emily B Derrick, Andrew Kueh, Ian J Majewski(Southern Biotech 4030-05), anti-mouse HRP (Southern Biotech 1070-05), and anti-goat HRP (Southern Biotech 4010-05). Immobilon Forte Chemiluminescent reagent (Millipore) was added to membranes prior to imaging with

(8) Expanding the toolbox to develop IAP-based degraders of TEAD transcription factorsCommunications ChemistryJanuary 19, 2026Nishma Gupta, Nicole Trainor, Mona Radwan, Stephanie Nguyen, Luke Duncan, Andrew X. Tang, Julia Beveridge, Natasha Silke, Jumana Yousef, Ceren Bilgilier, et al.goat anti-Rat IgG HRP (1:5000, Southern Biotech, 3010-05), goat Anti-Rabbit IgG HRP (1:5000, Southern Biotech, 4010-05), goat anti-Mouse HRP (1:5000, Southern Biotech, 1010-05). The membranes were developed using Immobilon

(9) Unconventional binding of Calmodulin to CHK2 kinase inhibits catalytic activityBiochemical JournalDecember 3, 2025Christopher R. Horne, Tingting Wang, Samuel N. Young, Toby A. Dite, Hunter G. Nyvall, Sushant Suresh, Katherine A. Davies, Abner Gonzalez Castro, Vineet Vaibhav, Lucy J. Mather J., et al.anti-mouse IgG (Southern Biotech 1010–05) and HRP-conjugated goat anti-rabbit IgG (Southern Biotech 4010–05). All secondary antibodies were used at a dilution of 1:10,000. Blots were visualised using Immobilon

(10) A Split Biotin Ligase Approach to Revealing Proteins Associated with Oligomeric Alpha-Synuclein During AggregationResearch SquareOctober 17, 2025Analiese R. Fernandes, Abigail P. Owen, Ayman H. Faroqi, Jannifer Lee, Gunveen S. Sachdeva, Dmytro Morderer, Cody Hoffmann, Benjamin Madden, Shuwen Zhang, Yingxue Ren, et al.being washed (3 × 5 min in TBS-T). Membranes were incubated with anti-rabbit-HRP (Southern Biotech, 4010–05) for anti-aSYN blots or anti-streptavidin-HRP (Southern Biotech, 7100–05) for anti-biotin blots for

(11) Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle β2‐adrenergic signallingThe Journal of PhysiologyOctober 1, 2025Morten Hostrup, Lukas Moesgaard, Mads Fischer, Kate Aiko Wickham, Mads Pleshardt, Andreas Breenfeldt Andersen, Jacob Bejder, Martin Thomassen, Jens J. Nielsen, Yvette Dehnes, et al.peroxidase‐conjugated rabbit anti‐sheep (dilution 1:5000; P0163; Dako, Glostrup, Denmark), goat anti‐rabbit IgM/IgG (4010‐05; SouthernBiotech, Birmingham, AL, USA) and goat anti‐mouse (dilution 1:5000; P0447; Dako). Statistical

(12) Biochemical properties of glycerol kinase from the hypersaline-adapted archaeon Haloferax volcaniiApplied and Environmental MicrobiologyAugust 20, 2025Karol M. Sanchez, Julie A. Maupin-Furlowsecondary antibody, goat anti-rabbit HRP-conjugated (Southern Biotech, Birmingham, AL, U.S. Cat. No. 4010-05), was diluted 1:10,000 in blocking buffer and incubated with the membrane for 1 h at RT. Following secondary

(13) Genome-Wide CRISPR Screening Reveals That mTOR Inhibition Initiates Ferritinophagy and Ferroptosis in Head and Neck CancerCancer ResearchAugust 15, 2025Keiichi Koshizuka, Xingyu Wu, Kuniaki Sato, Pham Thuy Tien Vo, Gosia M. Murawska, Tomohiko Ishikawa, Zhiyong Wang, Alfredo A. Molinolo, Edward A. Dennis, Cherie-Ann O. Nathan, et al.horseradish peroxidase–conjugated goat anti-rabbit IgG or goat anti-mouse IgG secondary antibody (#4010–05, RRID:AB_2632593 and #1010–05, RRID:AB_2728714, Southern Biotech) at a dilution of 1:10,000 in 5% milk-TBST

(14) NLRP3 inflammasome-driven hemophagocytic lymphohistiocytosis occurs independent of IL-1β and IL-18 and is targetable by BET inhibitorsSci AdvJuly 11, 2025Farzaneh Shojaee, Esmaeel Azadian, Min Xian Wong, Xiuquan Ma, James Rickard, Jiyi Pang, Catherine Hall, Andrew J. Kueh, Seth L. Masters, Inmaculada Rioja, et al.incubated in relevant horseradish peroxidase (HRP)–conjugated secondary antibodies (Southern Biotech, 4010-05) diluted 1:5000 to 10,000 in 5% skim milk TBS-T for 1 hour at room temperature. Membranes were then

(15) Preserved efficacy of lyophilized SARS-CoV-2 mRNA vaccine incorporating novel ionizable lipids after one year at 25 °CNPJ VaccinesJuly 1, 2025Elena Mata, Esther Broset, Carlos Matute, Andrei Stoian, Susana Adame, Teresa Alejo, Alexandre López, Beatriz Andrés, Juan Heredero, Diego de Miguel, et al.The membrane was washed and incubated with a goat anti-rabbit Ig, Human ads-HRP (Southern Biotech, 4010-05) as a secondary antibody. Colorimetric detection of the samples was performed with Pierce ECL Western Blotting Substrate

(16) Time-resolved mitochondrial screen identifies regulatory components of oxidative metabolismEMBO ReportsApril 29, 2025Marcos Zamora-Dorta, Sara Laine-Menéndez, David Abia, Pilar González-García, Luis C López, Paula Fernández-Montes, Enrique Calvo, Jesús Vázquez, José Antonio Enríquez, Eduardo Balsaresource,Source,Reference Antibodies,Antibodies,Antibodies NDUFA9,Proteintech,20312-1-AP NDUFS2,Abcam,ab192022 ACAD9,Proteintech,15770-1-AP SDHA,Invitrogen,459200 UQCRQ,Invitrogen,PA5-106446 MT-CO1,Abcam,ab14705 NDUFV1,Proteintech,11238-1-AP NDUFB7,Proteintech,14912-1-AP lipoic acid,Abcam,AB58724 Vinculin,Abcam,AB207440 Goat Anti-Rabbit Ig,Southern Biotech,4010-05 Goat Anti-Mouse Ig,Southern Biotech,1010-05 Goat anti-mouse Dylight 800,Invitrogen,SA5-10176 Alexa

(17) Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stressProceedings of the National Academy of SciencesFebruary 11, 2025Siyu Fan, Shuling Dong, Weijing Yao, Yi Zhang, Mingzhu Fan, Shan Feng, Choufei Wu, Liqin Zhang, Cong Yigoat anti-mouse IgG1 human ads-HRP (SouthernBiotech, 1070-05; 1:10,000), and goat anti-rabbit human ads-HRP (SouthernBiotech, 4010-05; 1:10,000). Western Blot and Immunoprecipitation. Yeast strains were grown to an OD600 of 0.8 ~ 1.2

(18) Fatiguing high-intensity intermittent exercise depresses maximal Na(+)-K(+)-ATPase activity in human skeletal muscle assessed using a novel NADH-coupled assayPflugers Archiv : European journal of physiologyFebruary 1, 2025Vigh-Larsen JF, Frangos SM, Overgaard K, Holloway GP, Mohr M.conjugated goat anti-mouse, rabbit anti-goat (P-0447 and P-0449 DAKO, Denmark), and goat anti-rabbit (4010–05 Southern Biotech, Birmingham AL, USA). Blood sampling and K+ analyses Blood was drawn in lithium-heparin

(19) Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screeningNat CommunJanuary 30, 2025Wei Jin, Yexuan Deng, John E. La Marca, Emily J. Lelliott, Sarah T. Diepstraten, Christina König, Lin Tai, Valentina Snetkova, Kristel M. Dorighi, Luke Hoberecht, et al.was washed ~3 times with PBS-T before incubation with HRP-conjugated anti-rabbit (Southern Biotech #4010-05) or anti-mouse (Southern Biotech #1010-05) secondary antibody. The protein bands were visualised by

(20) Effects of 8 Weeks of Moderate- or High-Volume Strength Training on Sarcoplasmic Reticulum Ca2+ Handling in Elite Female and Male RowersScand J Med Sci SportsJanuary 20, 2025Oscar Mazza, Joachim Nielsen, Jonas Mathiesen, Daniel Højme, Carsten Lundby, Morten Hostrup, Martin Thomassen, Peter Plomgaard, Kasper Degn Gejl, Niels ØrtenbladThermoFisher Scientific, 100 kDa; SERCA2: SC‐376235, Santa Cruz Biotechnology, 100 kDa. HRP conjugated goat anti‐rabbit (4010–05, SouthernBiotech), goat anti‐mouse (P0447, DAKO Denmark) and a rabbit anti‐goat (P0449, DAKO Denmark)

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Goat Anti-Rabbit Ig, Human ads-HRP from SouthernBiotech

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Goat Anti-Rabbit Ig, Human ads-HRP from SouthernBiotech

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