Goat Anti-Human IgG-AP 2040-04 from SouthernBiotech

Product Specs
ItemGoat Anti-Human IgG-AP
CompanySouthernBiotech
Price Pricing Info Supplier Page View Company Product Page
Catalog Number2040-04
Quantity1.0 mL
ApplicationsELISA, ELISPOT, IHC-FS, IHC-PS, ICC, WB
Conjugate/TagAP (Alkaline Phosphatase)
ReactivityHuman
TargetIgG
IsotypeIgG
HostGoat
FormatLiquid - 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
ClonalityPolyclonal
ConcentrationLot specific
PurityPooled antisera from goats hyperimmunized with human IgG; purified by affinity chromatography on human IgG covalently linked to agarose; reacts with the heavy chain of human IgG; cross-adsorbed against human IgM and IgA for minimal reactivity
ImmunogenHuman IgG
Research AreaImmunology, Secondary Antibodies, Stem Cells
Antibody TypeSecondary Antibody

Add to Compare List

SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 3Nature CommunicationsOctober 5, 2023Julie Elisabeth Heggelund, Saykat Das, Jorunn Stamnaes, Rasmus Iversen, Ludvig M. Sollidtemperature. After washing, the wells were incubated with alkaline phosphatase-conjugated goat anti-human IgG (2040-04, Southern Biotech) diluted 1:2000 for 1 h at room temperature, washed again and incubated with 5 mg

(2) Neutralization, effector function and immune imprinting of Omicron variantsNatureSeptember 1, 2023Addetia A, Piccoli L, Case JB, Park YJ, Beltramello M, Guarino B, Dang H, de Melo GD, Pinto D, Sprouse K, et al.Tween-20 (PBS-T) (Sigma-Aldrich, 93773), goat anti-human IgG-AP secondary antibody (Southern Biotech, 2040-04, diluted 1/500) was added and incubated for 1 h at room temperature. Plates were then washed four times

(3) Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralizationCell Chemical BiologyJuly 20, 2023Scott A. McConnell, Jaiprasath Sachithanandham, Nathan J. Mudrak, Xianming Zhu, Parsa Alba Farhang, Radames J.B. Cordero, Maggie P. Wear, Janna R. Shapiro, Han-Sol Park, Sabra L. Klein, et al.Binding Domain (RBD),Sino Biological,Cat#40150-D001 Goat α-Human IgG H + L-AP,Southern Biotech,Cat#2040-04; ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, SARS-CoV-2

(4) Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escapeiScienceJanuary 20, 2023Marzi R, Bassi J, Silacci-Fregni C, Bartha I, Muoio F, Culap K, Sprugasci N, Lombardo G, Saliba C, Cameroni E, et al.Tween20 (PBS-T), and binding was revealed using secondary goat anti-human IgG-AP (Southern Biotech, 2040-04). After washing, pNPP substrate (Sigma-Aldrich, 71768-25G) was added and plates were read at 405 nm

(5) Molecular states during acute COVID-19 reveal distinct etiologies of long-term sequelaeNature MedicineJanuary 1, 2023Ryan C. Thompson, Nicole W. Simons, Lillian Wilkins, Esther Cheng, Diane Marie Del Valle, Gabriel E. Hoffman, Carlo Cervia, Brian Fennessy, Konstantinos Mouskas, Nancy J. Francoeur, et al.temperature and washing, secondary antibody (goat anti-human IgG, IgA or IgM AP conjugate; SouthernBiotech, 2040-04, 2050-04 and 2020-04, respectively; diluted 1/4,500, 1/4,000 and 1/3,000, respectively) were added to

(6) Quality of antibody responses by adults and young children to 13-valent pneumococcal conjugate vaccination and Streptococcus pneumoniae colonisationVaccineNovember 28, 2022Asia-Sophia Wolf, Elena Mitsi, Scott Jones, Simon P. Jochems, Lucy Roalfe, Deus Thindwa, James E. Meiring, Jacquline Msefula, Farouck Bonomali, Tikhala Makhaza Jere, et al.temperature with goat anti-human IgG conjugated to alkaline phosphatase (Southern Biotech, cat. no. 2040–04) or horseradish peroxidase (Sigma, cat. no. AP112P). Plates were developed with p-nitrophenyl phosphate

(7) Rheumatoid factor IgM autoantibodies control IgG homeostasisFrontiers in ImmunologyOctober 21, 2022Antonella NicolÃ², Timm Amendt, Omal El Ayoubi, Marc Young, Stephanie Finzel, Makbule Senel, Reinhard E. Voll, Hassan JumaaAP, monoclonal, SouthernBiotech, #9020-04); anti-human IgG (Goat, AP, polyclonal, SouthernBiotech, #2040-04). HEp-2 slides and fluorescence microscopy Kallestad HEp-2 slides (BIO-RAD, #26101) were used to asses

(8) Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escapebioRxivOctober 20, 2022Roberta Marzi, Jessica Bassi, Chiara Silacci-Fregni, Istvan Bartha, Francesco Muoio, Katja Culap, Nicole Sprugasci, Gloria Lombardo, Christian Saliba, Elisabetta Cameroni, et al.Tween20 (PBS-T), and binding was revealed using secondary goat anti-human IgG-AP (Southern Biotech, 2040-04). After washing, pNPP substrate (Sigma-Aldrich, 71768–25G) was added and plates were read at 405 nm

(9) Imprinted antibody responses against SARS-CoV-2 Omicron sublineagesbioRxivSeptember 26, 2022Young-Jun Park et al.Tween 20 (PBS-T) (Sigma-Aldrich, 93773), goat anti-human IgG secondary antibody (Southern Biotech, 2040-04) was added and incubated for 1 h at room temperature. Plates were then washed four times with PBS-T

(10) Acute COVID-19 gene-expression profiles show multiple etiologies of long-term sequelaemedRxivSeptember 5, 2022Ryan C. Thompson et al.each well for 1 hour (SouthernBiotech #2020–04 Lot No. L4206-Q408B, #2050–04 Lot No. C5213-RI66P, #2040–04 Lot No. B3919-NE80C). Plates were washed three times with a wash buffer. Once dry, 100 μl SIGMAFAST

(11) Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicrybioRxivMay 26, 2022Young-Jun Park et al.Tween 20 (PBS-T) (Sigma-Aldrich, 93773), goat anti-human IgG secondary antibody (Southern Biotech, 2040–04) was added and incubated for 45 min at room temperature. Plates were then washed 4 times with PBS-T

(12) Humoral immunity against SARS‐CoV‐2 variants including omicron in solid organ transplant recipients after three doses of a COVID‐19 mRNA vaccineClinical & Translational ImmunologyApril 29, 2022Kapil K Saharia et al.above before incubation with secondary antibodies against pan‐human IgG (Southern Biotech, Cat. No. 2040‐04) or IgA (Southern Biotech, Cat. No. 2050‐04). Secondary antibodies were diluted according to the manufacturers’

(13) Structural basis for broad sarbecovirus neutralization by a human monoclonal antibodybioRxivApril 3, 2022M. Alejandra Tortorici et al.Tween 20 (PBS-T) (Sigma-Aldrich, 93773), goat anti-human IgG secondary antibody (Southern Biotech, 2040–04) was added and incubated for 1 h at room temperature. Plates were then washed 4 times with PBS-T and

(14) Antibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escapebioRxivApril 1, 2022Tyler N. Starr et al.Tween 20 (PBS-T) (Sigma-Aldrich, 93773), goat anti-human IgG secondary antibody (Southern Biotech, 2040–04) was added and incubated for 1 h at room temperature. Plates were then washed 4 times with PBS-T and

(15) Structural Basis for Broad HIV-1 Neutralization by a Novel MPER-Specific Human Broadly Neutralizing AntibodySSRN Electronic JournalApril 1, 2022Dora Pinto et al.Jackson Immunoresearch Cat#109-006-064; RRID:AB_2337548 AP-conjugated anti-human IgG secondary antibody Southern Biotech Cat#2040-04; RRID: AB_2795643 10E8 recombinant mAb IRB and Pasteur; Huang et al., 2012 PMID:23151583; RRID:AB_2491067 HK20

(16) Identification of polo-like kinase 1 as a therapeutic target in murine lupusClinical & translational immunologyJanuary 1, 2022Li Y, Wang H, Zhang Z, Tang C, Zhou X, Mohan C, Wu T.temperature. Bound Igs were detected with alkaline phosphatase‐conjugated goat anti‐mouse IgG (Catalog #2040‐04; Southern Biotech) or goat anti‐mouse IgM (Catalog #626822) from Invitrogen (Carlsbad, CA, USA), and

(17) Deep dissection of the antiviral immune profile of patients with COVID-19Communications BiologyDecember 16, 2021Djordje Atanackovic, Stephanie V. Avila, Forat Lutfi, Diego de Miguel-Perez, Xiaoxuan Fan, Gabriela Sanchez-Petitto, Erica Vander Mause, Jonathan Siglin, John Baddley, Heather D. Mannuel, et al.described above before incubation with secondary antibodies against pan-human IgG (Southern Biotech, cat # 2040-04), IgG1 (Southern Biotech, cat # 9052-04), IgG2 (Southern Biotech, cat # 9060-04), IgG3 (Southern Biotech,

(18) New-onset IgG autoantibodies in hospitalized patients with COVID-19Nature CommunicationsDecember 1, 2021Sarah Esther Chang et al.bound IgG was measured with goat anti-human IgG alkaline phosphatase conjugate (Southern Biotech, Cat # 2040-04) at a dilution of 1:2000. Samples were analyzed at dilutions of 1:30, 1:90, 1:270, and 1:810. Of these

(19) Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device supportScientific reportsNovember 16, 2021Ito T, Minamitani T, Hayakawa M, Otsubo R, Akiba H, Tsumoto K, Matsumoto M, Yasui T.mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich,

(20) Memory B cell repertoire for recognition of evolving SARS-CoV-2 spikeCellSeptember 16, 2021Tong P, Gautam A, Windsor IW, Travers M, Chen Y, Garcia N, Whiteman NB, McKay LGA, Storm N, Malsick LE, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Goat anti-human IgG-alkaline phosphatase (AP),Southern Biotech,Cat# 2040-04; RRID:AB_2795643 Streptavidin-alkaline phosphatase,BD Biosciences,"Cat# 554065, RRID:AB_10053566" Human

Write a Review

(1)

2/7/2017Anti-human IgG-AP (secondary Ab) for dsDNA ELISA Annie. KesslerWe routinely use Southern Biotech's anti-human IgG-alkaline phosphatase as the secondary antibody in an ELISA designed for detection of dsDNA. The antibody is consistent and reliable in our protocol ... Read Review

ELISA

Goat Anti-Human IgG-AP

ELISA

Goat Anti-Human IgG-AP from SouthernBiotech

SouthernBiotech

Citations (38)

Reviews

Goat Anti-Human IgG-AP from SouthernBiotech

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map