Frozen mouse lung tissue section following 21 days of hypoxia was stained with anti-S100A4 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-UNLB (SB Cat. No. 4050-01) followed by an AP conjugated secondary antibody and a substrate for the detection of alkaline phosphatase. Image from Kwapiszewska G, Wilhelm J, Wolff S, Laumanns I, Koenig IR, Ziegler A, et al. Expression profiling of laser-microdissected intrapulmonary arteries in hypoxia-induced pulmonary hypertension. Respir Res. 2005;6:109. Figure 5(b)