ELISA plate was coated with Goat Anti-Mouse IgG1, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG2a, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG2b, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG3, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG2a-BIOT (SB Cat. No. 0103-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.