Fig 1: [A, B] Immunohistology and statistical evaluation of colon samples from wild-type and TNFΔARE pigs showing an increase in Ki67+, CD3+ and IBA1+ cells and decrease in Pas/AB+ cells. Scale bars = 200 µm. [C, D] Confocal laser immunofluorescence scanning microscopy images of colonic sections stained for DAPI and ZO-1 or Occludin, respectively. Scale bars = 100 µm. Representative single cells were selected and shown magnified. Original signal intensities were adjusted for clear visualization of defined or diffuse antigen localization. [E, F] TNF mRNA levels and mRNA half-life assessed in macrophages from wild-type and TNFΔARE pigs. [G] Comparison of TNF transcript levels in colonic and ileal mucosal biopsy samples shown as multiples of the average wild-type value. [H] Comparison of TNF, IL-6 and IL-8 mRNA levels in intestinal samples shown as multiples of the average wild-type value on a log2 scale. Caec, caecum; Duod, duodenum; Jej, jejunum. [I, J] TNF and GAPDH protein expression in colonic [K] and ileal samples [L] from three wild-type, three TNFΔARE/+ and three TNFΔARE/ΔARE pigs. M, size marker.