FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgM-APC (SB Cat. No. 0101-11) was captured and fluorescence intensity quantified.