Fig 1: Alcohol impairs hepatic proteasome function and genetic inhibition of proteasome increases protein levels of hepatic RIP1 and RIP3WT mice were treated with Gao-binge model. A. Total liver lysates were subjected to western blot analysis. B. Densitometry analysis of (A). The levels of PSMA2, PSMC1 and PSMB5 were normalized to the loading control (β-Actin). Values represent means±S.E. (n = 3). C. Twenty micrograms of total liver lysates were used to measure the 20S proteasomal activities using a fluorogenic substrate. Results are presented as means± S.E. (n = 3, *p<0.05 by Student t test). D. Total liver lysates from PSMC1 flox/flox, albumin Cre negative (PSMC1 f/f) and PSMC1 flox/flox, albumin Cre positive (PSMC1 f/f Alb) were subjected to western blot analysis. E. Densitometry analysis of (D). Values represent means±S.E. (n = 3).