HMGB1 Antibody PA1-16926 from Thermo Fisher Scientific

Product Specs
ItemHMGB1 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-16926
NCBI Gene AliasesAc2-008, Hmg1, p30, HMG-1, HMG1, SBP-1, HMG3
ClonalityPolyclonal
HostRabbit
ReactivityBovine, Canine, Human, Mouse, Ovine, Rabbit, Rat
TargetHMGB1
ApplicationsChIP assay, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
FormatPBS with 0.02% sodium azide
Quantity100 µL
Concentration1 mg/mL
ImmunogenA synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200)
NCBI Full Gene Namehigh mobility group box 1
IsotypeIgG
Storage Temperature4° C, do not freeze
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage4°C, do not freeze

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(1) Targeting Melanin Heterogeneity in Metastatic Melanoma: A Dual‐Tumour Mouse Melanoma ModelExperimental DermatologySeptember 2, 2025Marine Delmas, Benjamin Chaussin, Nathan Harismendy, Aurore Dougé, Paul‐Olivier Rouzaire, Christopher Montemagno, Jérôme Durivault, Emmanuel Moreau, Elisabeth Miot‐Noirault, Mercedes Quintana, et al.The following primary antibodies were used: anti‐calreticulin (Abcam ab92516), anti‐HMGB1 (Invitrogen PA1‐16926), anti‐CD3 (Abcam ab5690), anti‐CD8a (Invitrogen 14–0808‐82). The following secondary antibodies were

(2) Nicotinamide mononucleotide promotes female germline stem cell proliferation by activating the H4K16ac-Hmgb1-Fyn-PLD signaling pathway through epigenetic remodelingCell BiosciApril 17, 2025Hong Zhou, Yujie Liu, Geng G. Tian, Ji WuPTM-101, PTM Biotech), H4 (1:4000, 16047-1-AP, Proteintech), H4K16ac (1:1000, 13534S, CST), HMGB1 (1:1000, PA1-16926, Invitrogen), Cyclin D1 (1:4000, ab134175, Abcam), Cyclin E1 (1:1000, 11554-1-AP, Proteintech), Cyclin

(3) Serine supplementation suppresses hypoxia-induced pathological retinal angiogenesisTheranosticsApril 9, 2025Hitomi Yagi, Chenxi Qiu, Yan Zeng, Myriam Boeck, Shen Nian, Chuck T. Chen, Jarrod C Harman, Taku Kasai, Jeff Lee, Victoria Hirst, et al.electrophoresis and transferred to 0.2 μm pore-size PVDF membranes. Primary antibodies against HMGB1 (1:1000, #PA1-16926, Invitrogen), HIF-1α (1:1000, #NB100-479, Novus Biologicals), phospho-STAT3 (Tyr705) (1:1000, #9131,

(4) Delta-opioid receptor signaling alleviates neuropathology and cognitive impairment in the mouse model of Alzheimer's disease by regulating microglia homeostasis and inhibiting HMGB1 pathwayAlzheimers Res TherFebruary 1, 2025Yuan Xu, Naiyuan Shao, Feng Zhi, Ronghua Chen, Yilin Yang, Jiahui Li, Ying Xia, Ya Penganti-mouse secondary antibody (Alexa Fluor 594) were purchased from Invitrogen (Cat: 11-0112-82, 14-0451-82, PA1-16926, R37117, R37115, USA). DAPI, Counting Kit-8, one step Terminal deoxynucleotidyl transferase dUTP nick-end

(5) Evaluation of HMGB1 Expression as a Clinical Biomarker for CholangiocarcinomaCancer Genomics - ProteomicsJanuary 3, 2025SUPAKAN AMONTAILAK, ATTAPOL TITAPUN, APINYA JUSAKUL, RAYNOO THANAN, PHONGSARAN KIMAWAHA, WASSANA JAMNONGKAN, MALINEE THANEE, PAPITCHAYA SIRITHAWAT, SONGPOL HAOHAN, ANCHALEE TECHASENbinding. Each section was incubated with primary antibody HMGB1 (1:100, Invitrogen, Carlsbad, CA, USA, #PA1-16926) at 4˚C overnight. Then, the sections were subjected to three washes with 1X PBS+0.1% tween20 and were

(6) The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseasesProceedings of the National Academy of Sciences of the United States of AmericaJune 25, 2024Kim H, Park HH, Kim HN, Seo D, Hong KS, Jang JG, Seo EU, Kim IY, Jeon SY, Son B, et al.TOX (catalog number 99036) or antibodies from Invitrogen that recognize human HMGB1 (catalog number PA1-16926). The high purity rTOX protein used in our experiments was obtained from Abcam (catalog number ab160644)

(7) Combination of cowpea mosaic virus (CPMV) intratumoral therapy and oxaliplatin chemotherapy†Materials AdvancesJune 7, 2024Miguel A. Moreno-Gonzalez, Zhongchao Zhao, Adam A. Caparco, Nicole F. Steinmetzanti-mouse Ki67 – 1 : 250 (Invitrogen Cat# MA5–14520), rabbit anti-mouse HMGB1 – 1 : 100 (Invitrogen Cat# PA1–16926), rabbit anti-mouse calreticulin – 1 : 100 (Cell Signaling Cat# 62304S), rat anti-mouse F4/80 – 1 : 100

(8) Eugenol Reduced ΜPO, CD45 and HMGB1 Expression and Attenuated the Expression of Leukocyte Infiltration Markers in the Intestinal Tissue in Biliopancreatic Duct Ligation-Induced Pancreatitis in RatsMedicina (Kaunas, Lithuania)December 30, 2023Oikonomou P, Nikolaou C, Papachristou F, Sovatzidis A, Lambropoulou M, Giouleka C, Kontaxis V, Linardoutsos D, Papalois A, Pitiakoudis M, et al.instructions. Tissue sections were incubated for 60 min with rabbit polyclonal HMGB1 (dilution 1:250, PA1-16926, Thermo Scientific, Waltham, MA, USA), mouse monoclonal CD45 (dilution 1:250, sc-53047, Santa Cruz,

(9) Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitorsCell stem cellJanuary 6, 2022Meharena HS, Marco A, Dileep V, Lockshin ER, Akatsu GY, Mullahoo J, Watson LA, Ko T, Guerin LN, Abdurrob F, et al.RRID:AB_2070987" H3K27me3,Abcam,"Cat# ab6002, RRID:AB_305237" HMGB1,Thermo Fisher Scientific,"Cat# PA1-16926, RRID:AB_2248274" LMNB1,Abcam,"Cat# ab16048, RRID:AB_10107828" p16INK4a,Thermo Fisher Scientific,"Cat#

(10) Pentoxifylline prevents epileptic seizure via modulating HMGB1/RAGE/TLR4 signalling pathway and improves memory in pentylenetetrazol kindling ratsClinical and experimental pharmacology & physiologyAugust 1, 2021Badawi GA, Shokr MM, Zaki HF, Mohamed AF.

(11) The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune ResponsesCancersApril 15, 2020Beltrán Hernández I, Angelier ML, Del Buono D'Ondes T, Di Maggio A, Yu Y, Oliveira S.(Sigma-Aldrich) for 15 min at room temperature. The primary antibody Rabbit anti- HMGB1 (Invitrogen, PA1-16926) was added for 1 h at room temperature (1:50 in in PBS + 2% BSA), followed by staining with the secondary

(12) Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in miceNature communicationsJanuary 10, 2020Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, Hou SX.Santa Cruz, Cat#sc-271667 IHC & IF: 1:100, Rabbit anti-HMGB1 Polyclonal Antibody, Thermo Fisher, Cat#PA1-16926 IHC & IF: 1:100, Mouse anti-human LAMP-1 Antibody (H4A3), Biolegend, Cat#328601 IHC & IF: 1:150, Rabbit

(13) A small bioactive glycoside inhibits epsilon toxin and prevents cell deathDisease models & mechanismsOctober 10, 2019Shivappagowdar A, Pati S, Narayana C, Ayana R, Kaushik H, Sah R, Garg S, Khanna A, Kumari J, Garg L, et al.section, and the coverslips were fixed with 2.5% paraffin. HMGB1 (1:500, Thermo Fisher Scientific, PA1-16926) was used as primary antibody followed by addition of anti-rabbit Alexa Fluor 594 (1:500, Thermo Fisher Scientific

(14) Expression Analysis of Autophagy Related Markers LC3B, p62 and HMGB1 Indicate an Autophagy-Independent Negative Prognostic Impact of High p62 Expression in Pulmonary Squamous Cell CarcinomasCancersAugust 21, 2018Langer R, Neppl C, Keller MD, Schmid RA, Tschan MP, Berezowska S.1:8000, citrate buffer, 100 ◦ C, 30 min; HMGB1 (Thermo scientific, Waltham, MA, USA, rabbit polyclonal, #PA116926): 1:800, citrate buffer, 100 ◦ C, 30 min. For visualization, the Bond Polymer Refine Detection kit (Leica

(15) Biomarkers of immunogenic stress in metastases from melanoma patients: Correlations with the immune infiltrateOncoimmunologyJune 1, 2016Ladoire S, Senovilla L, Enot D, Ghiringhelli F, Poirier-Colame V, Chaba K, Erdag G, Schaefer JT, Deacon DH, Zitvogel L, et al.

(16) The presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancerAutophagyMay 3, 2016Ladoire S, Enot D, Senovilla L, Ghiringhelli F, Poirier-Colame V, Chaba K, Semeraro M, Chaix M, Penault-Llorca F, Arnould L, et al.

(17) Negative prognostic impact of regulatory T cell infiltration in surgically resected esophageal cancer post-radiochemotherapyOncotargetAugust 28, 2015Vacchelli E, Semeraro M, Enot DP, Chaba K, Poirier Colame V, Dartigues P, Perier A, Villa I, Rusakiewicz S, Gronnier C, et al.Without washing, the primary antibody, a polyclonal rabbit anti HMGB1 antibody (ThermoScientist Pierce, PA1-16926) at the final concentration of 4µg/mL, was incubated overnight. After two washes in 0.025% Triton (v/v

(18) Combined evaluation of LC3B puncta and HMGB1 expression predicts residual risk of relapse after adjuvant chemotherapy in breast cancerAutophagyJanuary 1, 2015Ladoire S, Penault-Llorca F, Senovilla L, Dalban C, Enot D, Locher C, Prada N, Poirier-Colame V, Chaba K, Arnould L, et al.

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HMGB1 Antibody

HMGB1 Antibody from Thermo Fisher Scientific

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HMGB1 Antibody from Thermo Fisher Scientific

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