Western blot was performed using Anti-Myeloperoxidase Rabbit Polyclonal Antibody (Product # PA1-26402) and 87 kDa, 73 kDa and 60 kDa bands corresponding to MPO was observed across cell lines and tissues tested except K-562, HeLa and Mouse Spleen. Membrane enriched extracts (30 µg lysate) of HL-60 (Lane 1), HL-60 treated with Retinoic Acid (1 µM for 72 hr) (Lane 2), K-562 (Lane 3), HeLa (Lane 4), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 5) and Mouse Bone Marrow (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).