Villin (1D2C3) Antibody sc-58897 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemVillin (1D2C3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-58897
Antibody TypePrimary Antibodies
NCBI Gene AliasesVIL, D2S1471, Vil
Clone1D2C3
Quantity200 µg/ml
Reactivitymouse, rat, human, avian
ApplicationsWB, IP, IF, IHC(P), ELISA
ClonalityMonoclonal
FormatPurified
Conjugate/TagUnconjugated
Hostmouse
NCBI Full Gene Namevillin 1
TargetVillin
IsotypeIgG1

Questions and Answers

What applications has the Villin (1D2C3) antibody been used for in scientific research?

The Villin (1D2C3) antibody has been shown to work in various applications including Western blotting, immunofluorescence, immunohistochemistry, and ELISA, as evidenced by its use in studies such as those by Chen et al. (2021) and Matheson et al. (2016).

Can this antibody be used for studies involving intestinal organoids?

Yes, the Villin antibody has been utilized in research involving intestinal organoids. For instance, Nakanishi et al. (2022) reported its use in immunostaining to assess the expression of intestinal-specific markers in human induced pluripotent stem cell-derived intestinal organoids.

What species does the Villin antibody react with?

The Villin (1D2C3) antibody is reactive with multiple species, including humans, mice, rats, and chickens, making it versatile for various experimental models.

Are there any specific findings related to the use of this antibody in intestinal health studies?

Yes, the Villin antibody has been referenced in studies examining intestinal health, such as in the work by Momoh et al. (2024), where it was used to analyze epithelial cell maturation and differentiation in the context of congenital disorders affecting intestinal function.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Duck intestinal organoids: A novel model for waterfowl parvovirus infection and immune responses investigationPoultry ScienceApril 1, 2026Yunhang Zhang, Mengchun Ding, Longhai Ji, Tianmeng Shi, Kuan Zhao, Wuchao Zhang, Baishi Lei, Wanzhe YuanAntibody,Type,Supplier,Product Number ZO-1,Mouse,Invitrogen,33-9100 Lgr5,Rabbit,Invitrogen,PA5-23000 Villin,Mouse,Santa Cruz,SC-58897 Muc2,Rabbit,Abcam,ab134119 LYZ,Rabbit,Invitrogen,PA5-16668 List of antibodies used in this study.

(2) Proinflammatory cytokines modify Ca2+ signaling and induce an apoptotic lipidome in murine small intestinal organoidsFrontiers in PhysiologyFebruary 6, 2026Svenja Mareike Pauer, Alina Schindler, Parviz Ghezellou, Bernhard Spengler, Martin Diener, Jasmin Balloutcaspase 3,Rabbit,Cell signaling Technology (#9661),RRID:AB_2341188,1:500 Villin,Mouse,Santa cruz (#sc-58897; Lot: E0119),RRID:AB_2304475,1:100 Secondary antibodies,Secondary antibodies,Secondary antibodies,Secondary

(3) Protection of clove extract against Salmonella enteritidis-induced intestinal dysfunction in broilers through JAK2/STAT3-mediated stem cell activationPoultry ScienceFebruary 1, 2026Yuqing Feng, Yaxue Lv, Hang Chen, Zhujin Lu, Sheng Zhang, Chunqi GaoZen-Bioscience), anti-PCNA (#200947-2E1, Zen-Bioscience), anti-Olfm4 (#39141, Zen-Bioscience), anti-Villin (#SC-58897, Santa Cruz Biotechnology), anti-phospho-STAT3 (#AP0474, ABclonal), and FITC-conjugated secondary antibody

(4) Transcriptomic Profiling of Porcine Duodenal, Jejunal, and Ileal Organoids in Response to Porcine Epidemic Diarrhea VirusJournal of Animal Science and TechnologyJanuary 31, 2026Jiyoung Heo, Jae Han Park, Hyun Sung Park, Dong-Hoon Chae, Aaron Yu, Keonwoo Cho, Dong Ha Bhang, Yoo Yong Kim, Mi-Kyung Oh, Kyung-Rok YuProtein,Company,Application,Dilution ZO-1,Santa Cruz (sc-33725),ICC,1:100 Villin,Santa Cruz (sc-58897),ICC,1:100 Chromogranin A,Abcam (ab15160),ICC,1:100 Ki67,DAKO (M7240),ICC,1:100 PEDV N,AntibodySystem

(5) Loss of Intestinal Endosome-associated Protein Sorting Nexin 27 Disrupts Epithelial Barrier and Promotes InflammationCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2026Shreya Deb, Yongguo Zhang, Yueqing An, Yinglin Xia, Jun Sunby Dr Paul A. Slesinger39,Kindly provided by Dr Paul A. Slesinger39 Villin,Santa Cruz Biotechnology,sc-58897 ZO-1,Invitrogen,339100 ZO-2,Invitrogen,389100 NHE3,Kindly provided by Dr Arivarasu Natarajan Anbazhagan68,Kindly

(6) Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host–virus interactions during porcine coronavirus infectionVeterinary ResearchNovember 21, 2025Ludivine Percevault, Léon-Charles Tranchevent, Lionel Bigault, Maxime Berthaud, Damien Le Gloahec, Pierrick Lucas, Flora Carnet, Aurélie Le Roux, Gérald Le Diguerher, Frédéric Paboeuf, et al.given by VIM unit INRAE lab,1:500,Mouse Lysozyme,PA5-16668-USA-Invitrogen,1:100,Rabbit Villin 1,1D2C3-sc-58897-USA-Santa Cruz,1:300,Mouse Lgr5,PA5-23000- USA-Invitrogen,1:100,Rabbit ZO1,"R40-76, sc-33725-USA-Santa Cruz

(7) Eicosapentaenoic acid enhances intestinal stem cell-mediated colonic epithelial regeneration by activating the LSD1-WNT signaling pathwayJournal of Advanced ResearchNovember 1, 2025Dan Wang, Nianbang Wu, Pei Li, Xiaojuan Zhang, Wenshuai Xie, Shunkang Li, Ding Wang, Yanling Kuang, Shaokui Chen, Yulan Liuantibodies: Muc2 (No. sc-515032, Santa Cruz, 1:1000), ChgA (No. sc-393941, Santa Cruz, 1:1000), Villin (No. sc-58897, Santa Cruz, 1:1000), Lgr5 (No. A12327, ABclonal, 1:1000), Non-phospho (Active) β-catenin (No. A22180,

(8) Epac2 Deficiency Compromises Adaptation to Dietary Acidification by Decreasing H+ Transport in the Renal NephronFunction (Oxford, England)October 28, 2025Pyrshev K, Atamanchuk A, Yang W, Kordysh M, Mei F, Zaika O, Cheng X, Pochynyuk O.anti-villin conjugated with Alexa Fluor 546 (1:50, Santa Cruz Biotechnology Inc., Dallas, TX, USA; Cat. #sc-58897) antibodies overnight at +4°C. Nuclei were stained with DAPI (0.5 µg/mL) for 15 min at room temperature.

(9) A Self-Assembling Senolytic Prodrug with Enhanced Bioavailability and Selective Activation for Targeting Senescent Retinal Pigment EpitheliumBiomaterials ResearchOctober 1, 2025Haewon Ok, Hyun-Seo Park, Jungin Park, Sunyoung Hwang, Jiwon Jang, Jiye Kim, Gaeun Park, Dojoon Park, Tae-Eun Park, Chaekyu Kim, et al.Inc.) dissolved in Dulbecco’s PBS for 1 h. The samples were incubated with an anti-villin antibody (sc-58897, 1:100; Santa Cruz Biotechnology) overnight at 4 °C. The samples were subsequently incubated with goat

(10) Protocol for the establishment and characterization of South African patient-derived intestinal organoidsSTAR protocolsSeptember 19, 2025Abdulla N, Aronson R, Plessis TD, Bebington B, Kaur M.CA",Cat# NB500-170 Anti-human Villin (1D2C3) (1:50 dilution),"Santa Cruz Biotechnology, USA","Cat# SC-58897, RRID: AB_2304475" Anti-human ChgA (C-12) (1:50 dilution),"Santa Cruz Biotechnology, USA","Cat# SC-393941,

(11) miR-181a-5p mediates the effects of BMP4 on intestinal cell proliferation and differentiationCell Death DisMay 28, 2025Chang Li, Yuning Zhou, Zhijie Yin, Yinping Jiang, Jinpeng Liu, Heidi L. Weiss, Qingding Wang, B. Mark Eversanti-KRT20 (Abcam; ab854), anti-Na,K-ATPase (Cell Signaling; #3010), anti-VILLIN (Santa Cruz Biotechnology; sc-58897) and anti-Ki67 (R&D Systems, Minneapolis, MN; MAB7617), E-cadherin (Cell Signaling; #3195) and cyclin

(12) IL-1β Induced Intestinal Inflammation Pathogenesis in East Friesian Sheep: Insights from Organoid ModelingAnimals (Basel)April 10, 2025Yue Xue, Yulong Zhao, Shuo Yan, Ruilin Du, Huiming Zhang, Wenna Yao, Teligun Bao, Fei Pan, Siqin Bao, Xihe Li, et al.anti-TNF rabbit polyclonal antibody (D221347, BBI, 1:200 dilution, Markham, ON, Canada), mouse a-villin (sc-58897, Santa Cruz Biotechnology, 1:200 dilution, Dallas, TX, USA), and monoclonal mouse anti-β-catenin (c7207,

(13) Meprin β regulates osteopontin-signaling in ischemia/reperfusion-induced kidney injuryBMC NephrolFebruary 22, 2025Faihaa Ahmed, Shaymaa Abousaad, Ayman Abouzeid, Christine Adhiambo, Elimelda Moige Ongerivillin in βKO kidney sections (mouse monoclonal anti-villin antibodies, Santa Cruz Biotechnology Cat# sc-58897, RRID: AB_2304475) diluted 1:200 for 1 h at room temperature. The slides sections were then rinsed three

(14) A gut-on-a-chip incorporating human faecal samples and peristalsis predicts responses to immune checkpoint inhibitors for melanomaNature Biomedical EngineeringFebruary 12, 2025Mattia Ballerini, Serena Galiè, Punit Tyagi, Carlotta Catozzi, Hariam Raji, Amir Nabinejad, Angeli D. G. Macandog, Alessandro Cordiale, Bianca Ionela Slivinschi, Karol K. Kugiejko, et al.β-catenin (1:200, BD Bioscience, catalogue number ab8480), villin (1:100, Santa Cruz, catalogue number sc-58897), Ki67 (1:500, Abcam, catalogue number ab15580), cleaved caspase-3 (1:400, Cell Signaling, catalogue

(15) Inhibition of the Wnt/β‑catenin signaling pathway and SOX9 by XAV939 did not alleviate inflammation in a dextran sulfate sodium‑induced ulcerative colitis modelExperimental and therapeutic medicineFebruary 1, 2025Liang SJ, Wang K, Mao DB, Xie LW, Zhu DJ.ab234437; Abcam), SOX9 (cat. no. ab185230; Abcam), β-catenin (cat. no. R22820; Zenbio), Villin (cat. no. SC58897; Santa Cruz Biotechnology, Inc.) and PPAR-γ (cat. no. 16643-1-AP; Wuhan Sanying Biotechnology) was performed.

(16) Morin Reactivates Nrf2 by Targeting Inhibition of Keap1 to Alleviate Deoxynivalenol-Induced Intestinal Oxidative DamageInternational journal of molecular sciencesJanuary 27, 2025Zan G, He H, Wang X, Zhou J, Wang X, Yan H.(#200947-2E1, Zen Bioscience, Chengdu, China), p-Nrf2 (#381559, Zen Bioscience, Chengdu, China), Villin (#sc-58897, Santa Cruz, TX, USA), and β-actin (#600149, Zen BioScience, Chengdu, China), as well as anti-rabbit

(17) Viroporin activity is necessary for intercellular calcium signals that contribute to viral pathogenesisScience advancesJanuary 17, 2025Gebert JT, Scribano FJ, Engevik KA, Huleatt EM, Eledge MR, Dorn LE, Philip AA, Kawagishi T, Greenberg HB, Patton JT, et al.,Cell Signaling Technologies,3726,1:500 Primary,E-cadherin,Rabbit,GeneTex,1004433,1:200 Primary,Sec61β,Rabbit,Invitrogen,PA3-015,1:500 Primary,GAPDH,Mouse,Novus,NB-600-502,1:5000 "Primary, conjugated",Villin,Mouse,Santa Cruz Biotechnology,Sc-58897,1:500 Secondary,Antirabbit Alexa Fluor 488,Donkey,Rockland,611-741-127,1:1000 Secondary,Antirabbit

(18) Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheetsProceedings of the National Academy of Sciences of the United States of AmericaJanuary 7, 2025Wijnakker JJAPM, van Son GJF, Krueger D, van de Wetering WJ, Lopez-Iglesias C, Schreurs R, van Rijt F, Lim S, Lin L, Peters PJ, et al.Antibody,Conc. in PBS "Villin (Santa Cruz, sc-58897)","1:1,000" "Phalloidin 488 (Life Technologies, A12379)","1:1,000" "Phalloidin 647 (Sigma, 65906)","1:1,000" "EPCAM

(19) Histological Changes of the Mucosal Epithelium in the Chicken Intestine during Pre- and Post-Hatching StagesThe journal of poultry scienceJanuary 1, 2025Al Amin M, Alam MB, Hiramatsu K.Sections were incubated with mouse anti-villin monoclonal antibody against chicken villin (diluted 1:20, sc-58897, Santa Cruz Biotechnology, Dallas, TX, USA) for 24 h followed by a 24 h incubation with rabbit antiserum

(20) A mucus layer derived from porcine intestinal organoid air–liquid interface monolayer attenuates swine enteric coronavirus infection by antiviral activity of Muc2BMC BiologyDecember 23, 2024Ning Yang, Yang Li, Yifei Cai, Yuanyuan Liu, Yunhang Zhang, Yuguang Fu, Chen Tan, Luc Willems, Guangliang Liuenteropathogenic microorganisms. Methods Antibodies Mouse anti-chicken Villin monoclonal antibody (sc-58897) and mouse anti-human CGA monoclonal antibody (sc-393941) were purchased from Santa Cruz (Texas, USA).

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Villin (1D2C3) Antibody

Villin (1D2C3) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Villin (1D2C3) Antibody from Santa Cruz Biotechnology, Inc.

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