Fig 1: FBXL16 dephosphorylated cyclin D1 by regulating Akt1/GSK-3ß signaling pathway via binding PP2AB55a. (a) Western blot analyses of cyclin D1 and phosphor-cyclin D1 (T286) in Ishikawa/MPA with shNC and shFBXL16 cells. (b) Coimmunoprecipitation of E3 ligase and cyclin D1 in Ishikawa/MPA cells with the shRNA-NC or shFBXL16 group. (c) Coimmunoprecipitation of FBXL16, PP2A-A a/ß, PP2A-B55a, and PP2A-C in Ishikawa/MPA cells with the shRNA-NC or shFBXL16 group. (d) Western blot analyses of phosphor-cyclin D1 (T286) and GSK3ß/AKT1 pathway in Ishikawa/MPA with shNC and shFBXL16 cells. Results are presented as mean ± SD. *P < 0.05. **P < 0.01. ***P < 0.001.