His-Tag (AD1.1.10) Antibody from Santa Cruz Biotechnology, Inc.

Yes, the His-Tag (AD1.1.10) antibody has been successfully utilized in Western blotting applications, as evidenced by its use in various studies, including those that detected recombinant proteins using this antibody for immunoblotting (alvarez2013, chatterj2024, herschho2014).

The His-Tag antibody has been employed in immunofluorescence assays, as indicated in studies where it was used to visualize proteins in cellular contexts (melen2020).

The His-Tag antibody has been shown to work with various sample types, including bacterial and mammalian cell lysates, as demonstrated in studies involving recombinant proteins expressed in Escherichia coli and mammalian cells (ji2016, wang2021).

While specific protocols are not detailed in the provided information, general practices for using the His-Tag antibody include blocking membranes with BSA and using appropriate secondary antibodies for detection, as seen in multiple studies that utilized this antibody in Western blotting and immunofluorescence (gao2017, zhu2017).