Fig 2: Cofilin is involved in cyclosporine A (CsA)-induced inhibition of myocardin-related transcription factors-serum response factor (SRF) transcription activity. SRF transcription activity was measured by luciferase gene reporter assay in Lilly Laboratories Porcine Kidney-1 SRE exposed 24 h to: (A) S3-R 100 µg/mL; S3R + CsA. (n = 6); (B) LIMKi3 10 µmol/L; LIMKi3 + CsA. (n = 7). Mean ± SEM. One-sample t test for vs control comparison, One-way ANOVA plus Tukey's post-test for multiple condition comparison (*P < .01, ***P < .001)

Fig 3: Cyclosporine A (CsA), but not tacrolimus (Tac), induced changes in cofilin (CFL) concentration-to-actin. A, Western blot detection of formaldehyde-cross-linked CFL oligomers in Lilly Laboratories Porcine Kidney-1 lysates. B, Quantification of relative protein abundance of CFL oligomeric forms. Mean ± SEM. One-way ANOVA plus Dunnett's post-test (*P < .05, **P < .01). Drug condition: 5 µmol/L CsA, 0.05 µmol/L Tac. Exposure time: 24 h (n = 5)